Generation of functional multipotent keratinocytes from mouse induced pluripotent stem cells

Methods in Molecular Biology

Volumn 961, Issue , 2013, Pages 337-350

  Bilousova, Ganna ^a   Roop, Dennis R ^a  

^a UNIVERSITY OF COLORADO SCHOOL OF MEDICINE   (United States)

Author keywords

Bone morphogenetic protein 4; Differentiation; Induced pluripotent stem cells; IPS cells; Keratinocytes; Multipotent skin stem cells; Retinoic acid

Indexed keywords

BONE MORPHOGENETIC PROTEIN 4; RETINOIC ACID;

ANIMAL; ARTICLE; CELL DIFFERENTIATION; CELL LINE; CULTURE TECHNIQUE; CYTOLOGY; EMBRYOID BODY; KERATINOCYTE; METABOLISM; METHODOLOGY; MOUSE; PLURIPOTENT STEM CELL;

ANIMALS; BONE MORPHOGENETIC PROTEIN 4; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL LINE; EMBRYOID BODIES; INDUCED PLURIPOTENT STEM CELLS; KERATINOCYTES; MICE; TRETINOIN;

MLCS; MLOWN;

EID: 84879299932     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-227-8_22     Document Type: Article

References (13)

1. Takahashi K, Yamanaka S (2006) Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126:663-676
2. Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131:861-872
3. Bilousova G, Chen J, Roop DR (2011) Differentiation of mouse induced pluripotent stem cells into a multipotent keratinocyte lineage. J Invest Dermatol 131:857-864
4. Aberdam E, Barak E, Rouleau M, de LaForest S, Berrih-Aknin S, Suter DM, Krause KH, Amit M, Itskovitz-Eldor J, Aberdam D (2008) A pure population of ectodermal cells derived from human embryonic stem cells. Stem Cells 26:440-444
5. Metallo CM, Ji L, de Pablo JJ, Palecek SP (2008) Retinoic acid and bone morphogenetic protein signaling synergize to efficiently direct epithelial differentiation of human embryonic stem cells. Stem Cells 26:372-380
6. Bain G, Kitchens D, Yao M, Huettner JE, Gottlieb DI (1995) Embryonic stem cells express neuronal properties in vitro. Dev Biol 168:342-357
7. Gambaro K, Aberdam E, Virolle T, Aberdam D, Rouleau M (2006) BMP-4 induces a Smad-dependent apoptotic cell death of mouse embryonic stem cell-derived neural precursors. Cell Death Differ 13:1075-1087
8. Bickenbach JR, Chism E (1998) Selection and extended growth of murine epidermal stem cells in culture. Exp Cell Res 244:184-195
9. Hager B, Bickenbach JR, Fleckman P (1999) Long-term culture of murine epidermal keratinocytes. J Invest Dermatol 112:971-976
10. Lichti U, Anders J, Yuspa SH (2008) Isolation and short-term culture of primary keratinocytes, hair follicle populations and dermal cells from newborn mice and keratinocytes from adult mice for in vitro analysis and for grafting to immunodeficient mice. Nat Protoc 3: 799-810
11. Mills AA, Zheng B, Wang XJ, Vogel H, Roop DR, Bradley A (1999) p63 is a p53 homologue required for limb and epidermal morphogenesis. Nature 398:708-713
12. Koster MI, Kim S, Mills AA, DeMayo FJ, Roop DR (2004) p63 is the molecular switch for initiation of an epithelial stratification program. Genes Dev 18:126-131
13. Chapman S, Liu X, Meyers C, Schlegel R, McBride AA (2011) Human keratinocytes are efficiently immortalized by a Rho kinase inhibitor. J Clin Invest 120:2619-2626