Crystal structure of human Karyopherin β2 bound to the PY-NLS of Saccharomyces cerevisiae Nab2

Journal of Structural and Functional Genomics

Volumn 14, Issue 2, 2013, Pages 31-35

  Soniat, Michael ^a   Sampathkumar, Parthasarathy ^b   Collett, Garen ^a   Gizzi, Anthony S ^b   Banu, Radhika N ^b   Bhosle, Rahul C ^b   Chamala, Swetha ^b   Chowdhury, Sukanya ^b   Fiser, Andras ^b   Glenn, Alan S ^b   Hammonds, James ^b   Hillerich, Brandan ^b   Khafizov, Kamil ^b   Love, James D ^b   Matikainen, Bridget ^b   Seidel, Ronald D ^b   Toro, Rafael ^b   Rajesh Kumar, P ^b   Bonanno, Jeffery B ^b   Chook, Yuh Min ^a   Almo, Steven C ^b   more..

^a UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

^b ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

Author keywords

Importin; Karyopherin; Nab2; Nuclear import; Nuclear localization signal; Nuclear pore; Nucleocytoplasmic transport

Indexed keywords

HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN; KARYOPHERIN BETA; KARYOPHERIN BETA2; NAB2 PROTEIN; TYROSINE; UNCLASSIFIED DRUG;

ARTICLE; BINDING SITE; CRYSTAL STRUCTURE; HUMAN; NONHUMAN; NUCLEAR LOCALIZATION SIGNAL; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN DATA BANK; PROTEIN MOTIF;

AMINO ACID SEQUENCE; BETA KARYOPHERINS; BINDING SITES; CELL NUCLEUS; CRYSTALLOGRAPHY, X-RAY; HUMANS; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; MOLECULAR SEQUENCE DATA; NUCLEAR LOCALIZATION SIGNALS; NUCLEOCYTOPLASMIC TRANSPORT PROTEINS; RNA, MESSENGER; RNA-BINDING PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

EUKARYOTA; SACCHAROMYCES CEREVISIAE;

EID: 84879234011     PISSN: 1345711X     EISSN: 15700267     Source Type: Journal    
DOI: 10.1007/s10969-013-9150-1     Document Type: Article

References (17)

1. Weis K (2003) Regulating access to the genome: nucleocytoplasmic transport throughout the cell cycle. Cell 112:441-451
2. Chook Y, Suel K (2011) Nuclear import by karyopherin-βs: recognition and inhibition. Biochim Biophys Acta 1813:1593-1606
3. Xu D, Farmer A, Chook Y (2010) Recognition of nuclear targeting signals by Karyopherin-β proteins. Curr Opin Struc Bio 20:782-790
4. Lee B, Cansizoglu A, Suel K, Louis T, Zhang Z, Chook Y (2006) Rules for nuclear localization sequence recognition by Karyopherinβ2. Cell 126:544-558
5. Cansizoglu A, Lee B, Zhang Z, Fontoura B, Chook Y (2007) Structure-based design of a pathway-specific nuclear import inhibitor. Nat Struc Mol Bio 14:452-454
6. Zhang ZC, Chook YM (2012) Structural and energetic basis of ALS-causing mutations in the atypical proline-tyrosine nuclear localization signal of the Fused in Sarcoma protein (FUS). Proc Natl Acad Sci USA 109:12017-12021
7. Fasken M, Stewart M, Corbett A (2008) Functional significance of the interaction between the mRNA-binding protein, Nab2, and the nuclear pore-associated protein, Mlp1, in mRNA export. J Bio Chem 283:27130-27143
8. Suel K, Gu H, Chook Y (2008) Modular organization and combinatorial energetics of proline-tyrosine nuclear localizations signals. PLoS Biol 6:1253-1267
9. Lee D, Aitchison J (2009) Kap104-mediated nuclear import. Nuclear localization signals in mRNA-binding proteins and the role of Ran and RNA. J Bio Chem 274:29031-29037
10. Tran E, Zhou Y, Corbett A, Wente S (2007) The DEAD-box protein Dbp5 controls mRNA export by triggering specific RNA:protein remodeling events. Mol Cell 28:850-859
11. Cansizoglu AE, Chook YM (2007) Conformational heterogeneity of Karyopherin β2 is segmental. Structure 15:1431-1441
12. Minor W, Cymborowski M, Otwinowski Z, Chruszcz M (2006) HKL-3000: the integration of data reduction and structure solution - from diffraction images to an initial model in minutes. Acta Crystallogr D Biol Crystallogr 62:859-866
13. McCoy AJ, Grosse-Kunstleve RW, Adams PD, Winn MD, Storoni LC, Read RJ (2007) PHASER crystallographic software. J Appl Crystallogr 40:658-674
14. Collaborative Computing Project Number 4 (1994) The CCP4 suite: programs for protein crystallography. Acta Crystallogr D Biol Crystallogr 50:760-763
15. Emsley P, Cowtan K (2004) COOT: model-building tools for molecular graphics. Acta Crystallogr D Biol Crystallogr 60:2126-2132
16. Davis IW, Leaver-Fay A, Chen VB, Block JN, Kapral GJ, Wang X, Murray LW, Arendall III WB, Snoeyink J, Richardson JS, Richardson DC (2007) MolProbity: all-atom contacts and structure validation for proteins and nucleic acids. Nucl Acids Res 35:W375-W383 (http://molprobity.biochem.duke.edu/)
17. Imasaki T, Shimizu T, Hashimoto H, Hidaka Y, Kose S, Imamoto N, Yamada M, Sato M (2007) Structural basis for substrate recognition and dissociation by human tranportin 1. Mol Cell 28:57-67