Transient expression for functional gene analysis using Populus protoplasts

Plant Cell, Tissue and Organ Culture

Volumn 114, Issue 1, 2013, Pages 11-18

  Tan, Biyue ^a   Xu, Meng ^a   Chen, Ying ^a   Huang, Minren ^a  

^a NANJING FORESTRY UNIVERSITY   (China)

Author keywords

Enzyme cocktail; Mesophyll cells; PEG mediated transfection; Protein interaction; Western blotting

Indexed keywords

ENZYME COCKTAILS; MESOPHYLL CELLS; PEG-MEDIATED TRANSFECTION; PROTEIN INTERACTION; WESTERN BLOTTING;

ENZYMES; MOLECULAR BIOLOGY; PLANTS (BOTANY); PROTEINS;

GENE EXPRESSION;

POPULUS;

EID: 84879228630     PISSN: 01676857     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11240-013-0299-x     Document Type: Article

References (30)

1. Asai T, Tena G, Plotnikova J, Willmann MR, Chiu WL, Gomez-Gomez L, Boller T, Ausubel FM, Sheen J (2002) MAP kinase signalling cascade in Arabidopsis innate immunity. Nature 415: 977-983.
2. Axtell MJ, Staskawicz BJ (2003) Initiation of RPS2-specified disease resistance in Arabidopsis is coupled to the AvrRpt2-directed elimination of RIN4. Cell 112: 369-377.
3. 2+ sensor protein kinases. Nature 464: 418-422.
4. Bradshaw HD, Ceulemans R, Davis J, Stettler R (2000) Emerging model systems in plant biology: poplar (Populus) as a model forest tree. J Plant Growth Regul 19: 306-313.
5. Busov VB, Strauss SH, Pilate G (2010) Transformation as a tool for genetic analysis in Populus genetics and genomics of Populus. In: Jansson S, Bhalerao RP, Groover AT (eds), genetics and genomics of Populus, 1st edn. Springer, New York, pp 113-133.
6. Davey MR, Anthony P, Power JB, Lowe KC (2005) Plant protoplasts: status and biotechnological perspectives. Biotechnol Adv 23: 131-171.
7. Dekeyser RA, Claes B, De Rycke R, Habets ME, van Montagu M, Caplan AB (1990) Transient gene expression in intact and organized rice tissues. Plant Cell 2: 591-602.
8. Du N, Liu X, Li Y, Chen S, Zhang J, Ha D, Deng W, Sun C, Zhang Y, Pijut P (2012) Genetic transformation of Populustomentosa to improve salt tolerance. Plant Cell Tiss Org Cult 108(2): 181-189.
9. Faraco M, Di Sansebastiano GP, Spelt K, Koes RE, Quattrocchio FM (2011) One protoplast is not the other! Plant Physiol 156: 474-478.
10. Guo J, Morrell-Falvey JL, Labbé JL, Muchero W, Kalluri UC, Tuskan GA, Chen JG (2012) Highly efficient isolation of Populus mesophyll protoplasts and its application in transient expression assays. PLoS ONE 7: e44908.
11. He S, Shan W, Kuang JF, Xie H, Xiao YY, Lu WJ, Chen JY (2012) Molecular characterization of a stress-response bZIP transcription factor in banana. Plant Cell Tiss Org Cult: 1-15. doi: 10. 1007/s11240-012-0258-y.
12. Jansson S, Douglas CJ (2007) Populus: a model system for plant biology. Annu Rev Plant Biol 58: 435-458.
13. Jiang L, Wang J, Liu Z, Wang L, Zhang F, Liu GC, Zhong Q (2010) Silencing induced by inverted repeat constructs in protoplasts of Nicotiana benthamiana. Plant Cell Tiss Org Cult 100: 139-148.
14. Kang J, Ide Y, Sasaki S (1995) Isolation and culture of leaf protoplasts from in vitro subcultured Poplars: Populus tomentosa, Populus alba cv. Pyramidalis × Populus tomentosa and Populus maximowiczii × Populus plantierensis. Bull Tokyo Uni For 93: 59-63.
15. Larkin PJ (1976) Purification and viability determinations of plant protoplasts. Planta 128: 213-216.
16. Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15(3): 473-497.
17. Petřivalský M, Vaníčková P, Ryzí M, Navrátilová B, Piterková J, Sedlářová M, Luhová L (2012) The effects of reactive nitrogen and oxygen species on the regeneration and growth of cucumber cells from isolated protoplasts. Plant Cell Tiss Org Cult 108(2): 237-249.
18. Pitzschke A, Persak H (2012) Poinsettia protoplasts-a simple, robust and efficient system for transient gene expression studies. Plant Methods 8: 14.
19. Rouxel T, Balesdent MH (2010) Avirulence genes. In: Encyclopedia of Life Sciences (ELS). John Wiley & Sons. doi: 10. 1002/9780470015902. a00212672010.
20. Russell JA, McCown BH (1986) Techniques for enhanced release of leaf protoplasts in Populus. Plant Cell Rep 5: 284-287.
21. Sasamoto H, Hosoi Y, Ishii K, Sato T, Saito A (1989) Factors affecting the formation of callus from leaf protoplasts of Populus alba. J Jpn For Soc 71: 449-455.
22. Sheen J (2001) Signal transduction in maize and Arabidopsis mesophyll protoplasts. Plant Physiol 127: 1466-1475.
23. Sodhi A, Biswas SK (2002) Monocyte chemoattractant protein-1-induced activation of p42/44 MAPK and c-Jun in murine peritoneal macrophages: a potential pathway for macrophage activation. J Interferon Cytokine Res 22: 517-526.
24. Taylor G (2002) Populus: Arabidopsis for forestry. Do we need a model tree? Ann Bot 90: 681-689.
25. Tuskan GA, DiFazio S, Jansson S, Bohlmann J, Grigoriev I, Hellsten U, Putnam N, Ralph S, Rombauts S, Salamov A, Schein J, Sterck L, Aerts A, Bhalerao RR, Bhalerao RP, Blaudez D, Boerjan W, Brun A, Brunner A, Busov V, Campbell M, Carlson J, Chalot M, Chapman J, Chen GL, Cooper D, Coutinho PM, Couturier J, Covert S, Cronk Q, Cunningham R, Davis J, Degroeve S, Déjardin A, Depamphilis C, Detter J, Dirks B, Dubchak I, Duplessis S, Ehlting J, Ellis B, Gendler K, Goodstein D, Gribskov M, Grimwood J, Groover A, Gunter L, Hamberger B, Heinze B, Helariutta Y, Henrissat B, Holligan D, Holt R, Huang W, Islam-Faridi N, Jones S, Jones-Rhoades M, Jorgensen R, Joshi C, Kangasjärvi J, Karlsson J, Kelleher C, Kirkpatrick R, Kirst M, Kohler A, Kalluri U, Larimer F, Leebens-Mack J, Leplé JC, Locascio P, Lou Y, Lucas S, Martin F, Montanini B, Napoli C, Nelson DR, Nelson C, Nieminen K, Nilsson O, Pereda V, Peter G, Philippe R, Pilate G, Poliakov A, Razumovskaya J, Richardson P, Rinaldi C, Ritland K, Rouzé P, Ryaboy D, Schmutz J, Schrader J, Segerman B, Shin H, Siddiqui A, Sterky F, Terry A, Tsai CJ, Uberbacher E, Unneberg P, Vahala J, Wall K, Wessler S, Yang G, Yin T, Douglas C, Marra M, Sandberg G, van de Peer Y, Rokhsar D (2006) The genome of black cottonwood, Populus trichocarpa (Torr. & Gray). Science 313: 1596-1604.
26. Walter M, Chaban C, Schütze K, Batistic O, Weckermann K, Näke C, Blazevic D, Grefen C, Schumacher K, Oecking C, Harter K, Kudla J (2004) Visualization of protein interactions in living plant cells using bimolecular fluorescence complementation. Plant J 40: 428-438.
27. Weinthal D, Tzfira T (2009) Imaging protein-protein interactions in plant cells by bimolecular fluorescence complementation assay. Trends Plant Sci 14: 59-63.
28. Wu F, Shen S, Lee L, Lee SH, Chan MT, Lin CS (2009) Tape-Arabidopsis Sandwich-a simpler Arabidopsis protoplast isolation method. Plant Methods 5: 16.
29. Yoo S, Cho Y, Sheen J (2007) Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis. Nat Protoc 2: 1565-1572.
30. Zhang Y, Su J, Duan S, Ao Y, Dai J, Liu J, Wang P, Li Y, Liu B, Feng D, Wang J, Wang H (2011) A highly efficient rice green tissue protoplast system for transient gene expression and studying light/chloroplast-related processes. Plant Methods 7(1): 30.