Evaluation of the fluorescent probes Nile Red and 25-NBD-cholesterol as substrates for steroid-converting oxidoreductases using pure enzymes and microorganisms

FEBS Journal

Volumn 280, Issue 13, 2013, Pages 3109-3119

  Faletrov, Yaroslav V ^a   Frolova, Nina S ^a   Hlushko, Hanna V ^a   Rudaya, Elena V ^a   Edimecheva, Irina P ^a   Mauersberger, Stephan ^b   Shkumatov, Vladimir M ^a  

^a BELARUSIAN STATE UNIVERSITY   (Belarus)

^b DRESDEN UNIVERSITY OF TECHNOLOGY   (Germany)

Author keywords

25 N (7 nitrobenz 2 oxa 1,3 diazol 4 yl) methyl amino 27 norcholesterol (25 NBD cholesterol); cholesterol oxidase; cytochrome P450 17 hydroxylase 17,20 lyase (CYP17A1); Nile Red; recombinant yeasts

Indexed keywords

CHOLESTEROL DEHYDROGENASE; CHOLESTEROL OXIDASE; CYTOCHROME P450 REDUCTASE; FLUORESCENT DYE; NILE RED; OXIDOREDUCTASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; STEROID 17ALPHA MONOOXYGENASE; UNCLASSIFIED DRUG;

BINDING SITE; BREVIBACTERIUM; BREVIBACTERIUM STEROLICUM; CATALYSIS; CONFERENCE PAPER; CONTROLLED STUDY; DEALKYLATION; ENZYME ACTIVE SITE; ENZYME SUBSTRATE; MOLECULAR DOCKING; NONHUMAN; OXIDATION; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA; SACCHAROMYCES CEREVISIAE; YARROWIA LIPOLYTICA;

4-CHLORO-7-NITROBENZOFURAZAN; ALKYLATION; BACTERIAL PROTEINS; BREVIBACTERIUM; CATALYTIC DOMAIN; CHOLESTEROL; CHOLESTEROL OXIDASE; FLUORESCENT DYES; FUNGAL PROTEINS; HUMANS; KINETICS; MOLECULAR CONFORMATION; MOLECULAR DOCKING SIMULATION; NADPH-FERRIHEMOPROTEIN REDUCTASE; OXAZINES; OXIDATION-REDUCTION; OXIDOREDUCTASES; PSEUDOMONAS AERUGINOSA; RECOMBINANT PROTEINS; STEROID 17-ALPHA-HYDROXYLASE; SUBSTRATE SPECIFICITY;

BACTERIA (MICROORGANISMS); BREVIBACTERIUM STEROLICUM; MAMMALIA; PSEUDOMONAS AERUGINOSA; SACCHAROMYCES CEREVISIAE; YARROWIA LIPOLYTICA;

EID: 84879219475     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/febs.12265     Document Type: Conference Paper

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