Determination of Neisseria gonorrhoeae susceptibility to ciprofloxacin in clinical specimens from men using a real-time PCR assay

International Journal of Antimicrobial Agents

Volumn 42, Issue 1, 2013, Pages 63-67

  Magooa, M P ^a   Muller E E ^a   Gumede, L ^a   Lewis, D A ^a,b,c  

^a NATIONAL INSTITUTE FOR COMMUNICABLE DISEASES   (South Africa)

^b UNIVERSITY OF THE WITWATERSRAND   (South Africa)

^c UNIVERSITY OF CAPE TOWN   (South Africa)

Author keywords

Ciprofloxacin; Neisseria gonorrhoeae; Quinolone; Real time PCR; Resistance; Susceptibility

Indexed keywords

CIPROFLOXACIN;

AMPLICON; ANTIBIOTIC RESISTANCE; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL GENE; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; CLINICAL ARTICLE; CONTROLLED STUDY; DCMH GENE; DNA DETERMINATION; DNA EXTRACTION; DNA SEQUENCE; GONORRHEA; GYRA GENE; HUMAN; MALE; NEISSERIA GONORRHOEAE; NONHUMAN; PARC GENE; PERFORMANCE MEASUREMENT SYSTEM; PHENOTYPE; POINT MUTATION; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION;

ANTI-BACTERIAL AGENTS; CIPROFLOXACIN; DNA, BACTERIAL; DRUG RESISTANCE, BACTERIAL; GENES, BACTERIAL; GENOTYPE; GONORRHEA; HUMANS; MALE; MICROBIAL SENSITIVITY TESTS; MOLECULAR DIAGNOSTIC TECHNIQUES; MUTATION; NEISSERIA GONORRHOEAE; REAL-TIME POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA;

EID: 84879198585     PISSN: 09248579     EISSN: 18727913     Source Type: Journal    
DOI: 10.1016/j.ijantimicag.2013.02.026     Document Type: Article

References (15)

1. D.A. Lewis, L. Scott, M. Slabbert, S. Mhlongo, A. van Zijl, and M. Sello Escalation in the relative prevalence of ciprofloxacin-resistant gonorrhoea among men with urethral discharge in two South African cities: association with HIV seropositivity Sex Transm Infect 84 2008 352 355
2. S. Mhlongo, P. Magooa, E.E. Müller, N. Nel, F. Radebe, and E. Wasserman Etiology and STI/HIV coinfections among patients with urethral and vaginal discharge syndromes in South Africa Sex Transm Dis 37 2010 566 570
3. World Health Organization. Global incidence and prevalence of selectable curable sexually transmitted infections - 2008. Geneva, Switzerland: WHO; 2012. http://apps.who.int/iris/bitstream/10665/75181/1/9789241503839-eng.pdf [accessed 20.02.13].
4. D.A. Lewis, and E. Marumo Revision of the national guideline for first-line comprehensive management and control of sexually transmitted infections: what's new and why? S Afr J Epid Infect 24 2009 6 9
5. National Department of Health. First line comprehensive management and control of sexually transmitted infections (STIs). Protocol for the management of a person with a sexually transmitted infection according to the Essential Drug List. Pretoria, South Africa: National Department of Health; 2008.
6. V. Maseko, L. Gumede, I. Venter, and D. Lewis Establishment of a gonococcal antimicrobial surveillance programme (GASP) in Africa: challenges and lessons learnt 13th International Union against Sexually Transmitted Infections World Congress 15-17 October 2012, Melbourne, Australia 2012
7. J. Giles, J. Hardick, J. Yuenger, M. Dan, K. Reich, and J. Zenilman Use of Applied Biosystems 7900HT sequence detection system and Taqman assay for detection of quinolone-resistant Neisseria gonorrhoeae J Clin Microbiol 42 2004 3281 3283
8. Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing; twenty-second informational supplement. Document M100-S22. Wayne, PA: CLSI; 2012.
9. R.J. Belland, S.G. Morrison, C. Ison, and W.M. Huang Neisseria gonorrhoeae acquires mutations in analogous regions of gyrA and parC in fluoroquinolone-resistant isolates Mol Microbiol 14 1994 371 380
10. E. Lindbäck, M. Rahman, S. Jalal, and B. Wretlind Mutations in gyrA, gyrB, parC, and parE in quinolone-resistant strains of Neisseria gonorrhoeae APMIS 110 2002 651 657
11. E. Lindbäck, M. Unemo, M. Akhras, B. Gharizadeh, H. Fredlund, and N. Pourmand Pyrosequencing of the DNA gyrase gene in Neisseria species: effective indicator of ciprofloxacin resistance in Neisseria gonorrhoeae APMIS 114 2006 837 841
12. M. Tanaka, S. Sakuma, K. Takahashi, T. Nagahuzi, T. Saika, and I. Kobayashi Analysis of quinolone resistance mechanisms in Neisseria gonorrhoeae isolates in vitro Sex Transm Infect 74 1998 59 62
13. T.R. Shultz, J.W. Tapsall, and P.A. White Correlation of in vitro susceptibilities to newer quinolones of naturally occurring quinolone-resistant Neisseria gonorrhoeae strains with changes in GyrA and ParC Antimicrob Agents Chemother 45 2001 734 738
14. H.M. Palmer, H. Mallinson, R.L. Wood, and A.J. Herring Evaluation of the specificities of five DNA amplification methods for the detection of Neisseria gonorrhoeae J Clin Microbiol 41 2003 835 837
15. R. Gold, I. Goldschneider, M.L. Lepow, T.F. Draper, and M. Randolph Carriage of Neisseria meningitidis and Neisseria lactamica in infants and children J Infect Dis 137 1978 112 121