BAK multimerization for apoptosis, but not bid binding, is inhibited by negatively charged residue in the BAK hydrophobic groove

Molecular Cancer

Volumn 12, Issue 1, 2013, Pages

  Azad, Abul ^a   Storey, Alan ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

Apoptosis; BAK; BCL 2; BH3; Cytochrome c; DNA damage; Mitochondria; Tyrosine Phosphorylation

Indexed keywords

CASPASE 3; CYTOCHROME C; ETOPOSIDE; PROTEIN BAK; PROTEIN BAX; PROTEIN BID; TYROSINE;

APOPTOSIS; ARTICLE; CELL FRACTIONATION; CELL STRAIN HCT116; DIMERIZATION; ENZYME ACTIVATION; ENZYME RELEASE; HYDROPHOBICITY; IMMUNOPRECIPITATION; MITOCHONDRION; PROTEIN BINDING; PROTEIN DEPHOSPHORYLATION; PROTEIN EXPRESSION; PROTEIN MULTIMERIZATION; PROTEIN PHOSPHORYLATION; PROTEIN PROCESSING; PROTEIN PROTEIN INTERACTION; ULTRAVIOLET IRRADIATION; WILD TYPE;

AMINO ACIDS; APOPTOSIS; BCL-2 HOMOLOGOUS ANTAGONIST-KILLER PROTEIN; BH3 INTERACTING DOMAIN DEATH AGONIST PROTEIN; CASPASE 3; CELL LINE, TUMOR; CYTOCHROMES C; HUMANS; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; MITOCHONDRIA; MODELS, MOLECULAR; MUTATION; PHOSPHORYLATION; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN MULTIMERIZATION; PROTEIN TRANSPORT; TUMOR SUPPRESSOR PROTEIN P53; TYROSINE;

EID: 84879044416     PISSN: None     EISSN: 14764598     Source Type: Journal    
DOI: 10.1186/1476-4598-12-65     Document Type: Article

References (20)

1. Adams JM, Cory S. The Bcl-2 apoptotic switch in cancer development and therapy. Oncogene 2007, 26:1324-1337. 10.1038/sj.onc.1210220, 2930981, 17322918.
2. Wei MC, Zong WX, Cheng EH, Lindsten T, Panoutsakopoulou V, Ross AJ, Roth KA, MacGregor GR, Thompson CB, Korsmeyer SJ. Proapoptotic BAX and BAK: a requisite gateway to mitochondrial dysfunction and death. Science 2001, 292:727-730. 10.1126/science.1059108, 3049805, 11326099.
3. van Van Delft MF, Huang DC. How the Bcl-2 family of proteins interact to regulate apoptosis. Cell Res 2006, 16:203-213. 10.1038/sj.cr.7310028, 16474435.
4. Youle RJ, Strasser A. The BCL-2 protein family: opposing activities that mediate cell death. Nat Rev Mol Cell Biol 2008, 9:47-59. 10.1038/nrm2308, 18097445.
5. Griffiths GJ, Dubrez L, Morgan CP, Jones NA, Whitehouse J, Corfe BM, Dive C, Hickman JA. Cell damage-induced conformational changes of the pro-apoptotic protein Bak in vivo precede the onset of apoptosis. J Cell Biol 1999, 144:903-914. 10.1083/jcb.144.5.903, 2148192, 10085290.
6. Dewson G, Kratina T, Sim HW, Puthalakath H, Adams JM, Colman PM, Kluck RM. To trigger apoptosis, Bak exposes its BH3 domain and homodimerizes via BH3:groove interactions. Mol Cell 2008, 30:369-380. 10.1016/j.molcel.2008.04.005, 18471982.
7. Dewson G, Kratina T, Czabotar P, Day CL, Adams JM, Kluck RM. Bak activation for apoptosis involves oligomerization of dimers via their alpha6 helices. Mol Cell 2009, 36:696-703. 10.1016/j.molcel.2009.11.008, 19941828.
8. Kim H, Rafiuddin-Shah M, Tu HC, Jeffers JR, Zambetti GP, Hsieh JJ, Cheng EH. Hierarchical regulation of mitochondrion-dependent apoptosis by BCL-2 subfamilies. Nat Cell Biol 2006, 8:1348-1358. 10.1038/ncb1499, 17115033.
9. Korsmeyer SJ, Wei MC, Saito M, Weiler S, Oh KJ, Schlesinger PH. Pro-apoptotic cascade activates BID, which oligomerizes BAK or BAX into pores that result in the release of cytochrome c. Cell Death Differ 2000, 7:1166-1173. 10.1038/sj.cdd.4400783, 11175253.
10. Leu JI, Dumont P, Hafey M, Murphy ME, George DL. Mitochondrial p53 activates Bak and causes disruption of a Bak-Mcl1 complex. Nat Cell Biol 2004, 6:443-450. 10.1038/ncb1123, 15077116.
11. Mihara M, Erster S, Zaika A, Petrenko O, Chittenden T, Pancoska P, Moll UM. p53 has a direct apoptogenic role at the mitochondria. Mol Cell 2003, 11:577-590. 10.1016/S1097-2765(03)00050-9, 12667443.
12. Pietsch EC, Perchiniak E, Canutescu AA, Wang G, Dunbrack RL, Murphy ME. Oligomerization of BAK by p53 utilizes conserved residues of the p53 DNA binding domain. J Biol Chem 2008, 283:21294-21304. 10.1074/jbc.M710539200, 2475706,2475706, 18524770.
13. Llambi F, Moldoveanu T, Tait SW, Bouchier-Hayes L, Temirov J, McCormick LL, Dillon CP, Green DR. A unified model of mammalian BCL-2 protein family interactions at the mitochondria. Mol Cell 2011, 44:517-531. 10.1016/j.molcel.2011.10.001, 3221787, 22036586.
14. Fox JL, Ismail F, Azad A, Ternette N, Leverrier S, Edelmann MJ, Kessler BM, Leigh IM, Jackson S, Storey A. Tyrosine dephosphorylation is required for Bak activation in apoptosis. EMBO J 2010, 29:3853-3868. 10.1038/emboj.2010.244, 2989102, 20959805.
15. Fox J, Azad A, Ismail F, Storey A. "Licensed to kill": tyrosine dephosphorylation and Bak activation. Cell Cycle 2011, 10:598-603. 10.4161/cc.10.4.14793, 3174003, 21293187.
16. Azad A, Fox J, Leverrier S, Storey A. Blockade of the BAK hydrophobic groove by inhibitory phosphorylation regulates commitment to apoptosis. PLoS One 2012, 7:e49601. 10.1371/journal.pone.0049601, 3506661, 23189150.
17. Waterhouse NJ, Trapani JA. A new quantitative assay for cytochrome c release in apoptotic cells. Cell Death Differ 2003, 10:853-855. 10.1038/sj.cdd.4401263, 12815469.
18. Moll UM, Wolff S, Speidel D, Deppert W. Transcription-independent pro-apoptotic functions of p53. Curr Opin Cell Biol 2005, 17:631-636. 10.1016/j.ceb.2005.09.007, 16226451.
19. Leverrier S, Bergamaschi D, Ghali L, Ola A, Warnes G, Akgul B, Blight K, Garcia-Escudero R, Penna A, Eddaoudi A, Storey A. Role of HPV E6 proteins in preventing UVB-induced release of pro-apoptotic factors from the mitochondria. Apoptosis 2007, 12:549-560. 10.1007/s10495-006-0004-1, 17195958.
20. Dai H, Smith A, Meng XW, Schneider PA, Pang YP, Kaufmann SH. Transient binding of an activator BH3 domain to the Bak BH3-binding groove initiates Bak oligomerization. J Cell Biol 2011, 194:39-48. 10.1083/jcb.201102027, 3135403, 21727192.