Synthesis of [68Ga]Gallium Dota-(Tyr3)-Octreotide Acetate ([68Ga]-Dotatoc)

Radiochemical Syntheses: Radiopharmaceuticals for Positron Emission Tomography

Volumn 1, Issue , 2012, Pages 321-334

  Eidherr, Harald ^a   Girschele, Friedrich ^a   Mitterhauser, Markus ^a,b   Wadsak, Wolfgang ^a,b  

^a MEDICAL UNIVERSITY OF VIENNA   (Austria)

^b UNIVERSITY OF VIENNA   (Austria)

Author keywords

68Ga DOTATOC, physical, pharmacokinetic, as PET tracer; Gallium 68 radiolabeled DOTA (Tyr3) octreotide acetate (DOTATOC); Visualizing neuroendocrine tumors, overexpressing SSTR

Indexed keywords

EID: 84879011618     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1002/9781118140345.ch32     Document Type: Chapter

References (51)

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6. 68Ga]Gallium generator is produced by iThemba Labs, RSA, and is available from I.D.B. Holland B.V., Baarle-Nassau, The Netherlands.
7. 68Ga]Gallium generator is produced from Eckert & Ziegler Isotope Products, Burbank (CA), USA, and is also available from Eckert & Ziegler Eurotope GmbH, Berlin, Germany.
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12. Hydrochloric acid 0.1 M was purchased from Merck, Darmstadt, Germany, (part no. 1.09060.1000) and used as received.
13. Raytest SynChrom module, Straubenhardt, Germany.
14. Ethanol was purchased from Merck, Darmstadt, Germany, (part no. 1.00986.1000) and used as received.
15. Bidistilled water was purchased from Fresenius Kabi, Graz, Austria (part no.16.231) and used as received.
16. Sodium acetate was purchased from Fluka, Buchs, Switzerland (part no. 71188) and used as received.
17. 3)-octreotide acetate (DOTATOC) was purchased from Bachem, Bubendorf, Switzerland (part no. 4028058) and used as received.
18. Hydrochloric acid 0.05 M was prepared by diluting one part of 0.1 M hydrochloric acid [5] with one part of bidistilled water [8].
19. RP18 Light Sep-Pak cartridges were purchased from Waters, Milford, MA, USA (part no. WAT 023501) and conditioned with 10 mL ethanol [7] plus 10 mL air, and subsequently with 10 mL bidistilled water [8] plus 10 mL air.
20. Saline 0.9% was purchased from Noriderm Enterprises, Nicosia, Cyprus (part no. 1-28379), and used as received.
21. Sodium dihydrogenphosphate monohydrate was purchased from Merck, Darmstadt, Germany (part no. 1.06346.1000), and used as received.
22. Disodiumhydrogenphosphate dihydrate was purchased from Merck, Darmstadt, Germany (part no. 1.06576.1000), and used as received.
23. Millex GV Low Protein binding 0.22-μm filters were purchased from Millipore, Bedford, MA, USA (part no. SLGV 033RS), and used as received.
24. Sterile vials, 11 mL, were purchased from Mallinckrodt Medical, Petten, Holland (part no. DRN 4357), and used as received.
25. Millex GV low protein binding 0.22-μm filters were purchased from Millipore, Bedford, MA, USA (part no. SLGV 013SL), and used as received for the vented air needle.
26. Pharmacopoea Europea <6.0/0125>. Radioactive pharmaceuticals. Ph.Eur. 6; 2008.
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28. Merck Hitachi, Darmstadt, Germany.
29. Packard, Meriden, CT, USA.
30. Macherey-Nagel, Düren, Germany (part no. 720120.46), and used as received.
31. Trifluoroacetic acid was purchased from Fluka, Buchs, Switzerland (part no. 91700), and used as received.
32. Acetonitrile in HPLC grade was purchased from Merck, Darmstadt, Germany (part no. 1.00029.1000), and used as received.
33. ITLC SG glass fiber sheets were purchased from Pall Life Sciences, Ann Arbor, MI, USA (part no. P/N 61886).
34. Varian, Darmstadt, Germany, Chromatography paper ITLC SA.
35. Wunderlich G, Herrling P, Zürn A, Anders P, Kotzerke J. Nuklearmedizin 2007;49:73-77.
36. Sodium citrate was purchased from Fluka, Buchs, Switzerland (part no. 71402), and used as received.
37. Concentrated aqueous hydrochloric acid solution (32%) was purchased from Merck, Darmstadt, Germany (part no. 100319), and used as received.
38. Ammonium acetate was purchased from Fluka, Buchs, Switzerland (part no. 1.01116.0500), and used as received.
39. Methanol in HPLC grade was purchased from Merck, Darmstadt, Germany (part no. 106008), and used as received.
40. The Gita Star Gamma Isotope Thin Layer Analyzer was purchased from Raytest, Straubenhardt, Germany.
41. So far at our institution a Gita Star 5 mm 60-150 keV tungsten PET collimator (part no. 02900005) was used, which was from Raytest, Straubenhardt, Germany. Even better suitable would be a Gita Star 20 mm >450 keV tungsten PET collimator (part no. 02900008), which is available from the same supplier.
42. The WTW pH 537 pH meter was purchased from Weilheim i. OB, Germany.
43. The Wescor osmometer 5520 was purchased from Sanova Medical Systems, Vienna, Austria.
44. The Cobra II Auto-Gamma counter was purchased from Packard, Meriden, CT, USA.
45. The activimeter was a Capintec CRC-15R Radioisotope Dose Calibrator, Ramsey, NJ, USA.
46. The external laboratory that performed both endotoxin analyses and sterility testings was the research laboratory of the Department for Infectious Diseases and Tropical Medicine of the Medical University of Vienna, Vienna, Austria.
47. Pharmacopoea Europea <6.0/2.06.14.00>. Determination of Bacterial Endotoxins. Ph.Eur. 6; 2008.
48. U.S. Pharmacopeia <85>. Bacterial Endotoxins Test. USP 32-NF 27; 2009.
49. The Pyrochrome® Limulus Amoebocyte Lysate (LAL) Test was purchased from Cape Cod, East Falmouth, MA, USA.
50. Pharmacopoea Europea <6.0/2.06.01.00>. Determination of Sterility. Ph.Eur. 6; 2008.
51. U.S. Pharmacopeia <71>. Sterility Tests. USP 32-NF 27; 2009.