Assessing Fc glycan heterogeneity of therapeutic recombinant monoclonal antibodies using NP-HPLC

Methods in Molecular Biology

Volumn 988, Issue , 2013, Pages 169-180

  Raju, T Shantha ^a  

^a JANSSEN RESEARCH AND DEVELOPMENT   (Belgium)

Author keywords

ADCC; Anthranilic acid; Antibodies; C1q; CDC; Exoglycosidase; Fc R; Fluorescence; Fluorophore; Glycan; HPLC; IgGs; Mapping; Monoclonal; NP HPLC; PNGase F; Reductive amination

Indexed keywords

ANTHRANILIC ACID; FC RECEPTOR; GALACTOSE; GLYCAN; MONOCLONAL ANTIBODY; RECOMBINANT ANTIBODY; SIALIC ACID; GLYCOPEPTIDASE; IMMUNOGLOBULIN FC FRAGMENT; IMMUNOGLOBULIN G; POLYSACCHARIDE; RECOMBINANT PROTEIN;

ARTICLE; DERIVATIZATION; FLUORESCENCE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PRIORITY JOURNAL; PROTEIN PURIFICATION; CARBOHYDRATE ANALYSIS; CHEMISTRY; GLYCOSYLATION; HUMAN; ISOLATION AND PURIFICATION; MOLECULAR GENETICS; PROTEIN PROCESSING;

ANTIBODIES, MONOCLONAL; CARBOHYDRATE SEQUENCE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; GLYCOSYLATION; HUMANS; IMMUNOGLOBULIN FC FRAGMENTS; IMMUNOGLOBULIN G; MOLECULAR SEQUENCE DATA; PEPTIDE-N4-(N-ACETYL-BETA-GLUCOSAMINYL) ASPARAGINE AMIDASE; POLYSACCHARIDES; PROTEIN PROCESSING, POST-TRANSLATIONAL; RECOMBINANT PROTEINS;

EID: 84878898154     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-327-5_10     Document Type: Article

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