Unbiased characterization of the senescence-associated secretome using SILAC-based quantitative proteomics

Methods in Molecular Biology

Volumn 965, Issue , 2013, Pages 175-184

  Acosta, Juan Carlos ^a   Snijders, Ambrosius P ^a   Gil, Jesús ^a  

^a IMPERIAL COLLEGE LONDON   (United Kingdom)

Author keywords

Conditioned media; Mass spectrometry; Proteomics; SASP; Secreted factors; Senescence secretome; SILAC; SMS

Indexed keywords

AMINO ACID;

ARTICLE; CELL AGING; CELL CULTURE; CELL STRAIN HEK293; CHEMISTRY; CULTURE MEDIUM; CYTOLOGY; FIBROBLAST; GENETICS; HUMAN; ISOTOPE LABELING; MASS SPECTROMETRY; METABOLISM; METHODOLOGY; ONCOGENE; PROTEOMICS; SECRETION (PROCESS);

AMINO ACIDS; CELL AGING; CELLS, CULTURED; CULTURE MEDIA, CONDITIONED; FIBROBLASTS; HEK293 CELLS; HUMANS; ISOTOPE LABELING; MASS SPECTROMETRY; ONCOGENES; PROTEOMICS;

MLCS; MLOWN;

EID: 84878860910     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-239-1_11     Document Type: Article

References (14)

1. Kuilman T, Michaloglou C, Mooi WJ, Peeper DS (2010) The essence of senescence. Genes Dev 24:2463-2479
2. Campisi J, d'Adda di Fagagna F (2007) Cellular senescence: when bad things happen to good cells. Nat Rev Mol Cell Biol 8:729-740
3. Collado M, Serrano M (2010) Senescence in tumours: evidence from mice and humans. Nat Rev Cancer 10:51-57
4. Kuilman T, Peeper DS (2009) Senescencemessaging secretome: SMS-ing cellular stress. Nat Rev Cancer 9:81-94
5. Coppe JP, Desprez PY, Krtolica A, Campisi J (2010) The senescence-associated secretory phenotype: the dark side of tumor suppression. Annu Rev Pathol 5:99-118
6. Kuilman T, Michaloglou C, Vredeveld LC, Douma S, van Doorn R, Desmet CJ, Aarden LA, Mooi WJ, Peeper DS (2008) Oncogene-induced senescence relayed by an interleukin-dependent in flammatory network. Cell 133:1019-1031
7. Coppe JP, Patil CK, Rodier F, Sun Y, Munoz DP, Goldstein J, Nelson PS, Desprez PY, Campisi J (2008) Senescence-associated secretory phenotypes reveal cell-nonautonomous functions of oncogenic RAS and the p53 tumor suppressor. PLoS Biol 6:2853-2868
8. Acosta JC, O'Loghlen A, Banito A, Guijarro MV, Augert A, Raguz S, Fumagalli M, Da Costa M, Brown C, Popov N, Takatsu Y, Melamed J, d'Adda di Fagagna F, Bernard D, Hernando E, Gil J (2008) Chemokine signaling via the CXCR2 receptor reinforces senescence. Cell 133:1006-1018
9. Mann M (2006) Functional and quantitative proteomics using SILAC. Nat Rev Mol Cell Biol 7:952-958
10. Zanivan S, Krueger M, Mann M (2012) In vivo quantitative proteomics: the SILAC mouse. Methods Mol Biol 757:435-450
11. Barradas M, Anderton E, Acosta JC, Li S, Banito A, Rodriguez-Niedenfuhr M, Maertens G, Banck M, Zhou MM, Walsh MJ, Peters G, Gil J (2009) Histone demethylase JMJD3 contributes to epigenetic control of INK4a/ARF by oncogenic RAS. Genes Dev 23:1177-1182
12. Cox J, Mann M (2008) MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteomewide protein quantification. Nat Biotechnol 26:1367-1372
13. Cox J, Matic I, Hilger M, Nagaraj N, Selbach M, Olsen JV, Mann M (2009) A practical guide to the MaxQuant computational platform for SILAC-based quantitative proteomics. Nat Protoc 4:698-705
14. Shevchenko A, Tomas H, Havlis J, Olsen JV, Mann M (2006) In-gel digestion for mass spectrometric characterization of proteins and proteomes. Nat Protoc 1: 2856-2860