Two years' performance of an in-house ELISA for diagnosis of Legionnaires' disease: Detection of specific IgM and IgG antibodies against Legionella pneumophila serogroup 1, 3 and 6 in human serum

Journal of Microbiological Methods

Volumn 94, Issue 2, 2013, Pages 94-97

  Elverdal, P L ^a   Jorgensen C S ^a   Krogfelt, K A ^a   Uldum, S A ^a  

^a STATENS SERUM INSTITUT   (Denmark)

Author keywords

Culture; In house ELISA; Legionella pneumophila serogroup 1, 3 and 6; PCR; Sensitivity; Urinary antigen test

Indexed keywords

IMMUNOGLOBULIN G ANTIBODY; IMMUNOGLOBULIN M ANTIBODY;

ADULT; AGE DISTRIBUTION; AGED; ANTIBODY DETECTION; ARTICLE; BACTERIUM CULTURE; BLOOD ANALYSIS; DENMARK; DIAGNOSTIC TEST ACCURACY STUDY; DIAGNOSTIC VALUE; ENZYME IMMUNOASSAY; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; HUMAN; LEGIONELLA PNEUMOPHILA; LEGIONNAIRE DISEASE; MAJOR CLINICAL STUDY; MALE; POLYMERASE CHAIN REACTION; PREDICTIVE VALUE; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; SEX RATIO; TRAVEL; VIRUS PNEUMONIA;

CULTURE; IN-HOUSE ELISA; LEGIONELLA PNEUMOPHILA SEROGROUP 1, 3 AND 6; PCR; SENSITIVITY; URINARY ANTIGEN TEST;

ADOLESCENT; ADULT; AGED; AGED, 80 AND OVER; ANTIBODIES, BACTERIAL; CHILD; DIAGNOSTIC TESTS, ROUTINE; ENZYME-LINKED IMMUNOSORBENT ASSAY; FEMALE; HUMANS; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M; LEGIONELLA PNEUMOPHILA; LEGIONNAIRES' DISEASE; MALE; MIDDLE AGED; POLYMERASE CHAIN REACTION; YOUNG ADULT;

LEGIONELLA; LEGIONELLA PNEUMOPHILA; LEGIONELLA PNEUMOPHILA SEROGROUP 1;

EID: 84878852727     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2013.04.010     Document Type: Article

References (16)

1. Bangsborg J.M., Friis-Møller A., Rechnitzer C., Høiby N., Lind K. The E. coli immunosorbent as used in serodiagnosis of Legionella infections studied by crossed immunoelectrophoresis. APMIS 1988, 96:177-184.
2. Boshuizen H.C., Neppelenbroek S.E., van Vliet H., Schellekens J.F., Boer J.W., Peeters M.F., Spaendonck M.A. Subclinical Legionella infection in workers near the source of a large outbreak of Legionnaires disease. J. Infect. Dis. 2001, 184:515-518.
3. Elverdal P.L., Svarrer C.W., Jørgensen C.S., Skovsted I.C., Uldum S.A. Development and validation of ELISA for detection of antibodies to Legionella pneumophila serogroup 1, 3 and 6 in human sera. J. Microbiol. Meth. 2011, 86(3):298-303.
4. Fields B.S., Benson R.F., Besser R.E. Legionella and Legionnaires' disease: 25years of investigation. Clin. Microbiol. Rev. 2002, 15:506-526.
5. Gaia V., Fry N.K., Afshar B., Lück P.C., Meugnier H., Etienne J., Peduzzi R., Harrison T.G. Consensus sequence-based scheme for epidemiological typing of clinical and environmental isolates of Legionella pneumophila. J. Clin. Microbiol. 2005, 43(5):2047-2052.
6. Ginevra C., Barranger C., Ros A., Mory O., Stephan J.-L., Freymuth F., Joannès M., Pozzetto B., Grattard F. Development and evaluation of Chlamylege, a new commercial test allowing simultaneous detection and identification of Legionella, Chlamydophila pneumoniae, and Mycoplasma pneumoniae in clinical respiratory specimens by Multiplex PCR. J. Clin. Microbiol. 2005, 43:3247-3254.
7. Glick T.H., Gregg M.B., Berman B., Mallison G., Rhodes W.W., Kassanoff I. Pontiac fever. An epidemic of unknown etiology in a health department. I. Clinical and epidemiologic aspects. Am. J. Epidemiol. 1978, 107:149-160.
8. Helbig J.H., Uldum S.A., Lück P.C., Harrison T.G. Detection of Legionella pneumophila antigen in urine samples by the BinaxNOW® immunochromatographic assay and comparison with both Binax® Legionella Urinary Enzyme Immunoassay (EIA) and Biotest Legionella Urine Antigen EIA. J. Med. Microbiol. 2001, 50:509-516.
9. Helbig J.H., Uldum S.A., Lück P.C. Detection of Legionella pneumophila antigen in urine samples: detection of serogroups and monoclonal subgroups. Legionella 2002, 204-206. American Society for Microbiology, Washington, DC. R. Marre, Y.U. Kwaik (Eds.).
10. Joseph C.A., Ricketts K.D. Legionnaires' disease in Europe 2007-2008. Eurosurveillance 2010, 15:9-16.
11. Krøjgaard L.H., Krogfelt K.A., Albrechtsen H.J., Uldum S.A. Cluster of Legionnaires' disease in a newly built block of flats, Denmark, December 2008-January 2009. Eurosurveillance 2011, 16:11-17.
12. McDade J.E., Shepard C.C., Fraser D.W., Tsai T.R., Redus M.A., Dowdle W.R. Legionnaires' disease, isolation of bacterium and demonstration of its role in other respiratory disease. N. Engl. J. Med. 1977, 297:1197-1203.
13. Nagington J., Wreghitt T.G., Tobin J.O., Macrae A.D. The antibody response in Legionnaires' disease. J. Hyg. (Lond) 1979, 83:377-381.
14. Ratzow S., Gaia V., Helbig J.H., Fry N.K., Lück P.C. Addition of neuA, the gene encoding N-acylneuraminate cytidylyl transferase, increases the discriminatory ability of the consensus sequence-based scheme for typing Legionella pneumophila serogroup 1 strains. J. Clin. Microbiol. 2007, 45(6):1965-1968.
15. Surveillance report Annual Epidemiological Report on Communicable Diseases 2010: Legionnaires Disease (Legionellosis) 2010.
16. Uldum S.A., Mølbak K. PCR as a routine method for diagnosis of Legionnaires' disease. Legionella 2002, 213-215. American Society for Microbiology, Washington, DC. R. Marre, Y.U. Kwaik (Eds.).