Development and validation of ELISA for detection of antibodies to Legionella pneumophila serogroup 1, 3 and 6 in human sera

Journal of Microbiological Methods

Volumn 86, Issue 3, 2011, Pages 298-303

  Elverdal, Pernille L ^a   Svarrer, Christina W ^a   Jørgensen, Charlotte S ^a   Skovsted, Ian C ^a   Uldum, Søren A ^a  

^a STATENS SERUM INSTITUT   (Denmark)

Author keywords

In house ELISA; Legionella antibodies; Legionella pneumophila serogroup 1 3 and 6; Legionella serology; Sensitivity; Specificity

Indexed keywords

BACTERIAL ANTIGEN; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M;

ANTIBODY DETECTION; ARTICLE; BACTERIUM CULTURE; BLOOD ANALYSIS; CONTROLLED STUDY; DIAGNOSTIC VALUE; DISEASE COURSE; ENZYME LINKED IMMUNOSORBENT ASSAY; FALSE POSITIVE RESULT; HUMAN; IMMUNOFLUORESCENCE TEST; INTERMETHOD COMPARISON; LEGIONELLA PNEUMOPHILA; LEGIONNAIRE DISEASE; MAJOR CLINICAL STUDY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; URINALYSIS;

ANTIBODIES, BACTERIAL; ENZYME-LINKED IMMUNOSORBENT ASSAY; HUMANS; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M; IMMUNOLOGIC TESTS; LEGIONELLA PNEUMOPHILA; LEGIONNAIRES' DISEASE; LIPOPOLYSACCHARIDES; SENSITIVITY AND SPECIFICITY; SEROTYPING;

LEGIONELLA; LEGIONELLA PNEUMOPHILA; LEGIONELLA PNEUMOPHILA SEROGROUP 1;

EID: 79960645804     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2011.05.022     Document Type: Article

References (24)

1. Bangsborg J.M., Friis-Møller A., Rechnitzer C., Høiby N., Lind K. The E. coli immunosorbent as used in serodiagnosis of Legionella infections studied by crossed immunoelectrophoresis. APMIS. 1988, 96:177-184.
2. Collins M.T., Espersen F., Høiby N., Cho S.N., Friis-Møller A., Reif J.S. Cross-reactions between Legionella pneumophila (serogroup 1) and twenty-eight other bacterial species, including other members of the family Legionellaceae. Infect Immun. 1983, 3:1441-1456.
3. Darveau R.P., Hancock R.E.W. Procedure for isolation of bacterial lipopolysaccharides from both smooth and rough Pseudomonas aeruginosa and Salmonella typhimurium strains. Journal of Bacteriology. 1983, 155:831-838.
4. De Ory F., Echevarría J.M., Pelaz C., Téllez A., Mateo M.A., López J. Detection of specific IgM antibody in the investigation of an outbreak of pneumonia due to Legionella pneumophila serogroup 1. Clin Microbiol Infect. 2000, 6:64-68.
5. ECDC website http://www.ecdc.europa.eu/en/activities/surveillance/ELDSNet/Pages/Index.aspx.
6. Edelstein P. Laboratory diagnosis of legionnaires' disease; an update from 1984. Legionella, Current Status and Emerging Perspectives 1993, 7-11. American Society for Microbiology, Washington DC. J. Barbaree (Ed.).
7. Fields B.S., Benson R.F., Besser R.E. Legionella and Legionnaires' Disease: 25years of investigation. Clinical Microbiology Reviews. 2002, 15:506-526.
8. Finidori J.P., Raoult D., Bornstein N., Fleurette J. Study of cross-reaction between Coxiella burnetii and Legionella pneumophila using immunofluorescence assay and immunoblotting. Acta Virol. 1992, 5:459-465.
9. Ginevra C., Barranger C., Ros A., Mory O., Stephan J.L., Freymuth F., Joannès M., Pozzetto B., Grattard F. Development and Evaluation of Chlamylege, a New Commercial Test Allowing Simultaneous Detection and Identification of Legionella, Chlamydophila pneumoniae, and Mycoplasma pneumoniae in Clinical Respiratory Specimens by Multiplex PCR. Journal of Clinical Microbiology. 2005, 43:3247-3254.
10. Helbig J.H., Uldum S.A., Lück P.C., Harrison T.G. Detection of Legionella pneumophila antigen in urine samples by the BinaxNOW immunochromatographic assay and comparison with both Binax Legionella Urinary Enzyme Immunoassay (EIA) and Biotest Legionella Urine Antigen EIA. J. Med. Microbiol. 2001, 50:509-516.
11. Helbig J.H., Uldum S.A., Lück P.C., Harrison T.G. Detection of Legionella pneumophila antigen in urine samples: detection of serogroups and monoclonal subgroups. Legionella 2002, 204-206. American Society for Microbiology, Washington DC. R. Marre, Y.U. Kwaik (Eds.).
12. Joseph C.A., Ricketts K.D. Legionnaires' Disease in Europe 2007-2008. Eurosurveillance. 2010, 15:9-16.
13. Jürgens D., Fehrenbach F.J. Cross-Reacting Lipopolysaccharide Antigens in Legionella pneumophila serogroup 1 to 14. Infection and Immunity. 1995, 63:2180-2184.
14. Lück P.C., Schuppler M., Helbig J.H. Changes in the lag-1 locus of Legionella pneumophila serogroup 1 strains result in different lipopolysaccharides recougnized by monoclonal antibodies but do not influence virulence. Legionella 2002, 52-55. American Society for Microbiology, Washington DC. R. Marre, Y.U. Kwaik (Eds.).
15. Marshall L.E., Boswell T.C.J., Kudesia G. False positive legionella serology in campylobacter infection: campylobacter serotypes, duration of antibody response and elimination of cross-reactions in the indirect fluorescent antibody test. Epidemiol Infect. 1994, 112:347-357.
16. McDonough E.A., Barrozo C.P., Russell K.L., Metzgar D. A multiplex PCR for detection of Mycoplasma pneumoniae, Chlamydophila pneumoniae, Legionella pneumophila, and Bordetella pertussis in clinical specimens. Mol Cell Probes. 2005, 19:314-322.
17. Murray P.R., Rosenthal K.S., Kobayashi G.S., Pfaller M.A. Medical Microbiology 2002, Mosby, Inc., Chile. fourth ed.
18. Nagington J., Wreghitt T.G., Tobin J.O., Macrae A.D. The antibody response in Legionnaires' disease. J Hyg (Lond). 1979, 83:377-381.
19. Skovsted I.C., Kerrn M.B., Sonne-Hansen J., Sauer L.E., Nielsen A.K., Konradsen H.B., Petersen B.O., Nyberg N.T., Duus J.Ø. Purification and structure characterization of the active component in the pneumococcal enzyme-linked immunosorbent assays. Vaccine. 2007, 25:6490-6500.
20. Uldum S.A., Mølbak K. PCR as a Routine Method for Diagnosis of Legionnaires' Disease. Legionella 2002, 213-215. American Society for Microbiology, Washington DC. R. Marre, Y.U. Kwaik (Eds.).
21. Uldum S.A., Finne L., Mølbak K. Legionella pneumonia 2007. Statens Serum Institut 2008, EPI-News no 22.
22. Uldum S.A., St-Martin G., Mølbak K. Legionella pneumonia 2008. Statens Serum Institut 2009, EPI-News no 40.
23. Wilkinson H.W., Fikes B.J., Cruce D.D. Indirect immunofluorescence test for serodiagnosis of Legionaires' disease: evidence for serogroup diversity of Legionaires' disease bacterial antigens and for multiple specificity of human antibodies. J Clin Microbiol. 1979, 9:379-383.
24. Zimmerman S.E., French M.L., Allen S.D., Wilson E., Kohler R.B. Immunoglobulin M antibody titers in the diagnosis of Legionnaires disease. J Clin Microbiol. 1982, 16:1007-1011.