Purification and characterization of the human cysteine-rich S100A3 protein and its pseudo citrullinated forms expressed in insect cells

Methods in Molecular Biology

Volumn 963, Issue , 2013, Pages 73-86

  Kizawa, Kenji ^a   Unno, Masaki ^b   Takahara, Hidenari ^b   Heizmann, Claus W ^c  

^a KANEBO LTD   (Japan)

^b IBARAKI UNIVERSITY   (Japan)

^c UNIVERSITY OF ZURICH   (Switzerland)

Author keywords

Baculovirus; Calciumbinding; Disulfide bridge; EF hand; Insect cell; Peptidylarginine deiminase; S100A3; Zinc binding

Indexed keywords

CALCIUM BINDING PROTEIN; COMPLEMENTARY DNA; CYSTEINE RICH S100A3 PROTEIN; DISULFIDE; PROTEIN ARGININE DEIMINASE; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG; CITRULLINE; CYSTEINE; PROTEIN S 100; RECOMBINANT DNA; S100A3 PROTEIN, HUMAN;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; ANION EXCHANGE CHROMATOGRAPHY; BACULOVIRUS EXPRESSION SYSTEM; CARBOXY TERMINAL SEQUENCE; CITRULLINATION; COLUMN CHROMATOGRAPHY; CONTROLLED STUDY; DISULFIDE BOND; GENE AMPLIFICATION; INSECT CELL; INSECT CELL CULTURE; NONHUMAN; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PROCESSING; PROTEIN PURIFICATION; SIGNAL TRANSDUCTION; SITE DIRECTED MUTAGENESIS; SIZE EXCLUSION CHROMATOGRAPHY; SPODOPTERA FRUGIPERDA; VIRUS RECOMBINANT; VIRUS TITRATION; ANIMAL; ARTICLE; BACULOVIRUS; CHEMISTRY; GEL CHROMATOGRAPHY; GENE EXPRESSION; GENE VECTOR; GENETICS; HOMOLOGOUS RECOMBINATION; HUMAN; ION EXCHANGE CHROMATOGRAPHY; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; MUTAGENESIS; SF9 CELL; SPODOPTERA; WESTERN BLOTTING;

ANIMALS; BACULOVIRIDAE; BLOTTING, WESTERN; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, ION EXCHANGE; CITRULLINE; CLONING, MOLECULAR; CYSTEINE; DISULFIDES; DNA, COMPLEMENTARY; DNA, RECOMBINANT; GENE EXPRESSION; GENETIC VECTORS; HOMOLOGOUS RECOMBINATION; HUMANS; MUTAGENESIS; PROTEIN PROCESSING, POST-TRANSLATIONAL; RECOMBINANT PROTEINS; S100 PROTEINS; SF9 CELLS; SPODOPTERA;

EID: 84878763785     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-230-8_5     Document Type: Chapter

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