Characterization of the cysteine-rich calcium-binding S100A3 protein from human hair cuticles

Biochemical and Biophysical Research Communications

Volumn 299, Issue 5, 2002, Pages 857-862

  Kizawa, Kenji ^a   Troxler, Heinz ^b   Kleinert, Peter ^b   Inoue, Takafumi ^a   Toyoda, Masahiko ^c   Morohashi, Masaaki ^c   Heizmann, Claus W ^b  

^a KANEBO LTD   (Japan)

^b UNIVERSITY OF ZURICH   (Switzerland)

^c UNIVERSITY OF TOYAMA   (Japan)

Author keywords

2D gel electrophoresis; Calcium; EF hand; Electrospray mass spectrometry; Hair cuticle; Purification; S100A3

Indexed keywords

ACETIC ACID DERIVATIVE; CALCIUM BINDING PROTEIN; CYSTEINE; METHIONINE; PROTEIN; PROTEIN S100A3; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; BIOCHEMISTRY; CALCIUM BINDING; CONTROLLED STUDY; GEL ELECTROPHORESIS; HAIR; HUMAN; HUMAN TISSUE; ISOELECTRIC POINT; PRIORITY JOURNAL; PROTEIN ISOLATION; PROTEIN PURIFICATION; TANDEM MASS SPECTROMETRY;

CALCIUM-BINDING PROTEINS; CHROMATOGRAPHY, ION EXCHANGE; CYSTEINE; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; HAIR FOLLICLE; HUMANS; MALE; S100 PROTEINS; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION;

EID: 0036926561     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-291X(02)02744-4     Document Type: Article

References (18)

1. C.W. Heizmann, G. Fritz, B.W. Schafer, S100 proteins: Structure, functions, and pathology, Front Biosci. 7 (2002) d1356-1368.
2. D. Engelkamp, B.W. Schafer, M.G. Mattei, P. Erne, C.W. Heizmann, Six S100 genes are clustered on human chromosome 1q21: Identification of two genes coding for the two previously unreported calcium-binding proteins S100D and S100E, Proc. Natl. Acad. Sci. USA 90 (1993) 6547-6551.
3. U.G. Fohr, C.W. Heizmann, D. Engelkamp, B.W. Schafer, J.A. Cox, Purification and cation binding properties of the recombinant human S100 calcium-binding protein A3, an EF-hand motif protein with high affinity for zinc, J. Biol. Chem. 270 (1995) 21056-21061.
4. 2+-binding protein S100A3: Spectroscopic investigations of its transition metal ion complexes, and three-dimensional structural model, Biochim. Biophys. Acta 1448 (1998) 264-276.
5. G. Fritz, P.R. Mittl, M. Vasak, M.G. Grutter, C.W. Heizmann, The crystal structure of metal-free human EF-hand protein S100A3 at 1.7-Å resolution, J. Biol. Chem. 277 (2002) 33092-33098.
6. K. Kizawa, H. Uchiwa, U. Murakami, Highly-expressed S100A3, a calcium-binding protein, in human hair cuticle, Biochim. Biophys. Acta 1312 (1996) 94-98.
7. K. Kizawa, S. Tsuchimoto, K. Hashimoto, H. Uchiwa, Gene expression of mouse S100A3, a cysteine-rich calcium-binding protein, in developing hair follicle, J. Invest. Dermatol. 111 (1998) 879-886.
8. T. Takizawa, S. Arai, K. Kizawa, H. Uchiwa, I. Sasaki, T. Inoue, Ultrastructural localization of S100A3, a cysteine-rich, calcium binding protein, in human scalp hair shafts revealed by rapid-freezing immunocytochemistry, J. Histochem. Cytochem. 47 (1999) 525-532.
9. T. Inoue, I. Sasaki, M. Yamaguchi, K. Kizawa, Elution of S100A3 from hair fiber: new model for hair damage emphasizing the loss of S100A3 from cuticle, J. Cosmet. Sci. 51 (2000) 15-25.
10. J.A. Swift, B. Bews, The chemistry of human hair cuticle-I: a new method for the physical isolation of cuticle, J. Soc. Cosmet. Chem. 25 (1974) 13-22.
11. T. Inoue, K. Kizawa, M. Ito, Characterization of soluble protein extracts from keratinized tissues: Identification of ubiquitin universally distributed in hair, nail, and stratum corneum, Biosci. Biotechnol. Biochem. 65 (2001) 895-900.
12. H. Schagger, G. von Jagow, Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa, Anal. Biochem. 166 (1987) 368-379.
13. A. Gorg, C. Obermaier, G. Boguth, A. Harder, B. Scheibe, R. Wildgruber, W. Weiss, The current state of two-dimensional electrophoresis with immobilized pH gradients, Electrophoresis 21 (2000) 1037-1053.
14. T. Rabilloud, Proteome Research: Two-Dimensional Gel Electrophoresis and Identification Methods, Ed., Springer, Berlin, Heidelberg, New York, 2000.
15. H. Troxler, E. Wetzel, T. Kuster, C.W. Heizmann, Design and construction of a nanoelectrospray ion source for a triple quadrupole mass spectrometer, Rev. Sci. Instrum. 70 (1999) 2257-2261.
16. F. Guignard, J. Mauel, M. Markert, Phosphorylation of myeloid-related proteins MRP-14 and MRP-8 during human neutrophil activation, Eur. J. Biochem. 241 (1996) 265-271.
17. M.J. Raftery, C.A. Harrison, P. Alewood, A. Jones, C.L. Geczy, Isolation of the murine S100 protein MRP14 (14 kDa migration-inhibitory-factor-related protein) from activated spleen cells: Characterization of post-translational modifications and zinc binding, Biochem. J. 316 (1996) 285-293.
18. P. Roepstorff, J. Fohlman, Proposal for a common nomenclature for sequence ions in mass spectra of peptides, Biomed. Mass Spectrom. 11 (1984) 601.