Nano-HPLC-MS of glycopeptides obtained after nonspecific proteolysis

Methods in Molecular Biology

Volumn 951, Issue , 2013, Pages 113-127

  Zauner, Gerhild ^a   Koeleman, Carolien A M ^a   Deelder, André M ^a   Wuhrer, Manfred ^a  

^a LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

Glycopeptides; Graphitized Carbon; HILIC; LC MS; Mass spectrometry; Reverse phase

Indexed keywords

CARBON; GLYCOPEPTIDE; PROTEINASE K; IMMOBILIZED ENZYME;

ARTICLE; ENZYME IMMOBILIZATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; HYDROPHILIC INTERACTION CHROMATOGRAPHY; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; NANOCHEMISTRY; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN GLYCOSYLATION; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; TANDEM MASS SPECTROMETRY; CHEMISTRY; ENZYME SPECIFICITY; METABOLISM; METHODOLOGY; NANOTECHNOLOGY; PROTEIN DATABASE; SOLUTION AND SOLUBILITY;

CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DATABASES, PROTEIN; ENDOPEPTIDASE K; ENZYMES, IMMOBILIZED; GLYCOPEPTIDES; NANOTECHNOLOGY; PROTEOLYSIS; SOLUTIONS; SUBSTRATE SPECIFICITY; TANDEM MASS SPECTROMETRY;

MLCS; MLOWN;

EID: 84878641622     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-146-2_9     Document Type: Article

References (17)

1. Hashimoto R, Hirose K, Sato T, Fukushima N, Miura N, Nishimura S (2010) Functional network of glycan-related molecules: glyconet in glycoconjugate data bank. BMC Syst Biol 4:91
2. Rek A, Krenn E, Kungl AJ (2009) Therapeutically targeting protein-glycan interactions. Br J Pharmacol 157:686-694
3. Varki A, Cummings R, Esko JD, Freeze HH, Hart GW, Marth J (1999) Essentials of glycobiology. Cold Spring Harbour Laboratory Press, Cold Spring Harbor, NY
4. Zauner G, Koeleman CA, Deelder AM, Wuhrer M (2010) Protein glycosylation analysis by HILIC-LC-MS of proteinase K-generated N- and O-glycopeptides. J Sep Sci 33:903-910
5. Dodds ED, Seipert RR, Clowers BH, German JB, Lebrilla CB (2009) Analytical performance of immobilized pronase for glycopeptide footprinting and implications for surpassing reductionist glycoproteomics. J Proteome Res 8:502-512
6. Wuhrer M, Koeleman CA, Hokke CH, Deelder AM (2005) Protein glycosylation analyzed by normal-phase nano-liquid chromatography- mass spectrometry of glycopeptides. Anal Chem 77:886-894
7. Dalpathado DS, Desaire H (2008) Glycopeptide analysis by mass spectrometry. Analyst 133: 731-738
8. Mysling S, Palmisano G, Hojrup P, Thaysen- Andersen M (2010) Utilizing ion-pairing hydrophilic interaction chromatography solid phase extraction for efficient glycopeptide enrichment in glycoproteomics. Anal Chem 82:5598-5609
9. Wuhrer M, Deelder AM, Hokke CH (2005) Protein glycosylation analysis by liquid chromatography- mass spectrometry. J Chromatogr B 825:124-133
10. Ruhaak LR, Deelder AM, Wuhrer M (2009) Oligosaccharide analysis by graphitized carbon liquid chromatography-mass spectrometry. Anal Bioanal Chem 394:163-174
11. Ruhaak LR, Zauner G, Huhn C, Bruggink C, Deelder AM, Wuhrer M (2010) Glycan labeling strategies and their use in identification and quantification. Anal Bioanal Chem 397:3457-3481
12. Mechref Y, Novotny MV (2002) Structural investigations of glycoconjugates at high sensitivity. Chem Rev 102:321-369
13. Zaia J (2004) Mass spectrometry of oligosaccharides. Mass Spectrom Rev 23:161-227
14. Morelle W, Michalski J-C (2005) The mass spectrometric analysis of glycoproteins and their glycan structures. Curr Anal Chem 1:29-57
15. Harvey DJ (1999) Matrix-assisted laser desorption/ionization mass spectrometry of carbohydrates. Mass Spectrom Rev 18:349-450
16. Wuhrer M, de Boer AR, Deelder AM (2009) Structural glycomics using hydrophilic interaction chromatography (HILIC) with mass spectrometry. Mass Spectrom Rev 28: 192-206
17. Viseux N, Costello CE, Domon B (1999) Post-source decay mass spectrometry: optimized calibration procedure and structural characterization of permethylated oligosaccharides. J Mass Spectrom 34:364-376