Bacillus subtilis endospores at high purity and recovery yields: Optimization of growth conditions and purification method

Current Microbiology

Volumn 66, Issue 3, 2013, Pages 279-285

  Tavares, Milene B ^a   Souza, Renata D ^a   Luiz, Wilson B ^a   Cavalcante, Rafael C M ^a   Casaroli, Caroline ^a   Martins, Eduardo G ^a   Ferreira, Rita C C ^a   Ferreira, Luís C S ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACILLUS SUBTILIS; BACTERIAL ENDOSPORE; BACTERIAL GROWTH; CONTROLLED STUDY; INTERMETHOD COMPARISON; NONHUMAN; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PRODUCT RECOVERY; PURIFICATION; QUALITATIVE ANALYSIS; QUANTITATIVE ANALYSIS; SPOROGENESIS;

BACILLUS SUBTILIS; CULTURE MEDIA; SPORES, BACTERIAL; TIME FACTORS;

BACILLUS SUBTILIS;

EID: 84878404635     PISSN: 03438651     EISSN: 14320991     Source Type: Journal    
DOI: 10.1007/s00284-012-0269-2     Document Type: Article

References (13)

1. Cutting SM (2011) Bacillus probiotics. Food Microbiol 28(2):214-220
2. Dragon DC, Rennie RP (2001) Evaluation of spore extraction and purification methods for selective recovery of viable Bacillus anthracis spores. Lett Appl Microbiol 33:100-105
3. Ferreira LCS, Ferreira RC, Schumann W (2005) Bacillus subtilis as a tool for vaccine development: from antigen factories to delivery vectors. An Acad Bras Cienc 77:113-124
4. Foerster HF, Foster JW (1966) Endotrophic calcium, strontium, and barium spores of Bacillus megaterium and Bacillus cereus. J Bacteriol 91:1333-1345
5. Hageman JH, Shankweiler GW, Wall PR, Franich K, McCowan GW, Cauble SM, Grajeda J, Quinones C (1984) Single, chemically defined sporulation medium for Bacillus subtilis: growth, sporulation, and extracellular protease production. J Bacteriol 160:438-441
6. Harrold ZR, Hertel MR, Gorman-Lewis D (2011) Optimizing Bacillus subtilis spore isolation and quantifying spore harvest purity. J Microbiol Methods 87(3):325-329
7. Klämpfl TG, Isbary G, Shimizu T, Li YF, Zimmermann JL, Stolz W, Schlegel J, Morfill GE, Schmidt HU (2012) Cold atmospheric air plasma sterilization against spores and other microorganisms of clinical interest. Appl Environ Microbiol 78(15):5077-5082
8. Laflamme C, Lavigne S, Ho J, Duchaine C (2004) Assessment of bacterial endospore viability with fluorescent dyes. J Appl Microbiol 96(4):684-692
9. Luiz WB, Cavalcante RCM, Paccez JD, Souza RD, Sbrogio-Almeida ME, Ferreira RRC, Ferreira LCS (2008) Boosting systemic and secreted antibody responses in mice orally immunized with recombinant Bacillus subtilis strains following parenteral priming with a DNA vaccine encoding the enterotoxigenic Escherichia coli (ETEC) CFA/I fimbriae B subunit. Vaccine 26:3998-4005
10. Monteiro SM, Clemente JJ, Henriques AO, Gomes RJ, Carrondo MJ, Cunha AE (2005) A procedure for high-yield spore production by Bacillus subtilis. Biotechnol Prog 21:1026-1031
11. Nicholson WL, Munakata H, Horneck G, Melosh HJ, Setlow P (2000) Resistance of Bacillus endospores to extreme terrestrial and extraterrestrial environments. Microbiol Mol Biol Rev 64:548-572
12. Nicholson WL, Setlow P (1990) Sporulation, germination and outgrowth. In: Harwood CR, Cutting SM (eds) Molecular biological methods for Bacillus. John Wiley & Sons Ltd., Chichester, pp 391-450
13. Paccez JD, Nguyen HD, Luiz WB, Ferreira RCC, Sbrogio-Almeida ME, Schumann W, Ferreira LCS (2005) Evaluation of different promoter sequences and antigen sorting signals on the immunogenicity of Bacillus subtilis vaccine vehicles. Vaccine 25:4671-4680