In vitro metabolic studies using homogenized horse liver in place of horse liver microsomes

Drug Testing and Analysis

Volumn 3, Issue 6, 2011, Pages 393-399

  Wong, Jenny K Y ^a   Tang, Francis P W ^a   Wan, Terence S M ^a  

^a Sha Tin Racecourse   (China)

Author keywords

Homogenized horse liver; In vitro; Metabolism; Microsomes; Steroids

Indexed keywords

AIR PURIFICATION; GAS CHROMATOGRAPHY; LIPIDS; MASS SPECTROMETRY; METABOLITES; PHYSIOLOGY;

HIGH SPEED; HOMOGENIZED HORSE LIVER; IN-PHASE; IN-VITRO; IN-VIVO; LIVER MICROSOMES; MICROSOME; STEROID; VITRO AND IN VIVO; VIVO METHODS;

METABOLISM;

CLOSTEBOL ACETATE; DRUG METABOLITE; EPITESTOSTERONE; METENOLONE ACETATE; TESTOSTERONE;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; IN VITRO STUDY; IN VIVO STUDY; LIVER HOMOGENATE; LIVER MICROSOME; MASS FRAGMENTOGRAPHY; NONHUMAN; ORGAN PRESERVATION; PRIORITY JOURNAL; TISSUE AND ORGAN PREPARATION AND CULTURE;

EID: 79959537775     PISSN: 19427603     EISSN: 19427611     Source Type: Journal    
DOI: 10.1002/dta.273     Document Type: Article

References (24)

1. P. W. Tang, W. C. Law, T. S. M. Wan, D. L. Crone. Metabolic studies of some oral anabolic steroids in horses: Part 1. oxymetholone and mestanolone, in Proceedings of the 12th International Conference of Racing Analysts and Veterinarians, (Eds: B. Laviolette, M. R. Koupai-Abyazani), R&W Publications: Newmarket, UK, 2000, pp. 118-124.
2. P. W. Tang, K. L. Watkins, T. S. M. Wan. Metabolic studies of oral anabolic steroids in horses. Part 2: Danazol, in Proceedings of the 13th International Conference of Racing Analysts and Veterinarians, (Eds: R. B. Williams, E. Houghton, J. F. Wade), R&W Publications: Newmarket, UK, 2001, pp. 171-178.
3. E. N. M. Ho, D. K. K. Leung, T. S. M. Wan, N. H. Yu. Metabolic studies of methenolone acetate in horses. Anal. Chim. Acta 2005, 540, 111.
4. G. N. W. Leung, E. N. M. Ho, D. K. K. Leung, F. P. W. Tang, T. S. M. Wan, J. H. K. Yeung, H. N. C. Wong. Metabolic studies of clostebol acetate in horses. Chromatographia, 2005, 61, 397.
5. E. N. M. Ho, W. H. Kwok, D. K. K. Leung, T. S. M. Wan, A. S. Y. Wong. Metabolic studies of turinabol in horses. Anal. Chim. Acta 2007, 586, 208.
6. E. N. M. Ho, D. K. K. Leung, G. N. W. Leung, T. S. M. Wan, H. N. C. Wong, X. Xu, J. H. K. Yeung. Metabolic studies of mesterolone in horses. Anal. Chim. Acta 2007, 596, 149.
7. G. N. W. Leung, F. P. W. Tang, T. S. M. Wan, C. H. F. Wong, K. K. H. Lam, B. D. Stewart. In vitro and in vivo studies of androst-4-ene-3, 6, 17-trione in horses by gas chromatography-mass spectrometry. Biomed. Chromatogr. 2010, 24, 744.
8. J. P. Scarth, H. A. Spencer, S. E. Timbers, S. C. Hudson, L. L. Hillyer. The use of in vitro technologies coupled with high resolution accurate mass LC-MS for studying drug metabolism in equine drug surveillance. Drug Testing Anal. 2010, 2, 1.
9. E. F. A. Brandon, C. D. Raap, I. Meijerman, J. H. Beijnen, J. H. M. Schellens. An update on in vitro test methods in human hepatic drug biotransformation research: pros and cons. Toxicol. Appl. Pharmacol. 2003, 189, 233.
10. M. M. Asaad, H. Barry, D. E. Clarke, B. N. Dixit. Effect of ethanol on the oxidative metabolism of tryptamine by rat liver homogenate. Brit. J. Pharmacol. 1974, 50, 277.
11. H. Shah, S. P. Hartman, S. Weinhouse. Formation of carbonyl chloride in carbon tetrachloride metabolism by rat liver in vitro. Cancer Res. 1979, 39, 3942.
12. 14C]benzylhydrazine in the rat. Drug Metab. Dispos. 1979, 7, 388.
13. J. Tjørnelund, S. H. Hansen, C. Cornett. New metabolites of the drug 5-aminosalicylic acid. I: N-β-D-glucopyanosyl-5-aminosalicylic acid. Xenobiotica 1989, 19, 891.
14. K. Shanmuganathan, T. Koudriakova, S. Nampalli, J. Du, J. M. Gallo, R. F. Schinazi, C. K. Chu. Enhanced brain delivery of an anti-HIV nucleoside 2'-F-ara-ddI by xanthine oxidase mediated biostransformation. J. Med. Chem. 1994, 37, 821.
15. R. A. Newman, A. Fuentes, J. M. Covey, J. A. Benvenuto. Preclinical pharmacology of the natural marine product dolastatin 10 (NSC 376128). Drug Metab. Dispos. 1994, 22, 428.
16. R. M. Crooke, M. J. Graham, M. J. Martin, K. M. Lemonidis, T. Wyrzykiewiecz, L. L. Cummins. Metabolism of antisense oligonucleotides in rat liver homogenates. J. Pharmacol. Exp. Ther. 2000, 292, 140.
17. A. Marwah, P. Marwah, H. Lardy. Ergosteroids VI. Metabolism of dehydroepiandrosterone by rat liver in vitro: a liquid chromatographic-mass spectrometric study. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 2002, 767, 285.
18. D. N. Bailey. Cocapropylene (propylcocaine) formation by human liver in vitro. J. Anal. Toxicol. 1995, 19, 1.
19. S. Ammon, O. von Richter, U. Hofmann, K. P. Thon, M. Eichelbaum, G. Mikus. In vitro interaction codeine and diclofenac. Drug Metab. Dispos. 2000, 28, 1149.
20. A. Machon, M. J. North, N. C. Price, D. E. Wells. The in vitro metabolism of Eulan WA New by liver homogenates from freshwater fish. Comp. Biochem. Physiol. C 1986, 84C, 113.
21. W. Schänzer, G. Opfermann, M. Donike. 17-Epimerization of 17α-methyl anabolic steroids in humans: metabolism and synthesis of 17α-hydroxy-17β-methyl steroids. Steroids 1992, 57, 537.
22. E. Houghton, M. C. Dumasia. Studies related to the metabolism of anabolic steroids in the horse: testosterone. Xenobiotica 1979, 9, 269.
23. E. Houghton, Anabolic steroids in the horse-a review of current knowledge, in Proceedings of the 9th International Conference of Racing Analysts and Veterinarians, (Ed: C. R. Short), R&W Publications: New Orleans, 1992, pp. 3-16.
24. ILAC-G7:6/2009. Accreditation Requirements and Operating Criteria for Horseracing Laboratories. Available at:.