Addition of zinc to human ejaculate prior to cryopreservation prevents freeze-thaw-induced DNA damage and preserves sperm function

Journal of Assisted Reproduction and Genetics

Volumn 29, Issue 12, 2012, Pages 1447-1453

  Kotdawala, Aditi P ^a   Kumar, Sangeetha ^a   Salian, Sujith R ^a   Thankachan, Prashanth ^b   Govindraj, Kaushik ^b   Kumar, Pratap ^a   Kalthur, Guruprasad ^a   Adiga, Satish K ^a  

^a KASTURBA MEDICAL COLLEGE   (India)

^b ST JOHN'S NATIONAL ACADEMY OF HEALTH SCIENCES   (India)

Author keywords

Acrosome reaction; Capacitation; Mitochondrial potential; Semen cryopreservation; Sperm DNA integrity; Zinc

Indexed keywords

CITRIC ACID; GLYCEROL; LIQUID NITROGEN; ZINC;

ACROSOME REACTION; ADULT; APTITUDE; ARTICLE; CRYOPRESERVATION; DNA DAMAGE; EGG YOLK; FREEZE THAWING; HUMAN; HUMAN CELL; MAJOR CLINICAL STUDY; MALE; MITOCHONDRIAL MEMBRANE POTENTIAL; PRIORITY JOURNAL; SEMEN ANALYSIS; SPERM; SPERMATOZOON; SPERMATOZOON MOTILITY;

CRYOPRESERVATION; CRYOPROTECTIVE AGENTS; DNA DAMAGE; GLYCEROL; HUMANS; MALE; SEMEN PRESERVATION; SPERMATOZOA; ZINC;

EID: 84878374608     PISSN: 10580468     EISSN: 15737330     Source Type: Journal    
DOI: 10.1007/s10815-012-9894-8     Document Type: Article

References (41)

1. Critser JK, Huse-Benda AR, Aaker DV, Arneson BW, Ball GD. Cryopreservation of human spermatozoa. The effect of cryoprotectants on motility. Fertil Steril. 1988;50:314-20.
2. Zribi N, Chakroun NF, EI Euch H, Gargouri J, Bahloul A, Keskes LA. Effects of cryopreservation on human sperm deoxyribonucleic acid integrity. Fertil Steril. 2010;93:159-66.
3. O'Connell M, McClure N, Lewis SE. The effect of cryopreservation on sperm morphology, motility and mitochondrial function. Hum Reprod. 2002;17:704-9.
4. Donnelly ET, McClure N, Lewis SEM. Cryopreservation of human semen and prepared sperm: effects on motility parameters and DNA integrity. Fertil Steril. 2001;76:892-900.
5. Donnelly ET, Steele KE, McClure N, Lewis SEM. Assessment of DNA integrity and morphology of ejaculated spermatozoa from fertile and infertile men before and after cryopreservation. Hum Reprod. 2001;16:1191-9.
6. Yoshida H, Hoshiai H, Fukaya T, Yajima A. Fertilization of fresh and frozen spermatozoa. Assist Reprod Technol Androl. 1990;1:164-72.
7. Bakos HW, Thompson JG, Feil D, Lane M. Sperm DNA damage is associated with assisted reproductive technology pregnancy. Int J Androl. 2008;31:518-26.
8. Thomson LK, Fleming SD, Aitken RJ, De Iuliis GN, Zieschang JA, Clark AM. Cryopreservation-induced human sperm DNA damage is predominantly mediated by oxidative stress rather than apoptosis. Hum Reprod. 2009;24:2061-70.
9. Lopes S, Jurisicova A, Sun JG, Casper RF. Reactive oxygen species: potential cause for DNA fragmentation in human spermatozoa. Hum Reprod. 1998;13:896-900.
10. Twigg J, Fulton N, Gomez E, Irvine DS, Aitken RJ. Analysis of the impact of intracellular reactive oxygen species generation on the structural and functional integrity of the human spermatozoa: lipid peroxidation, DNA fragmentation and effectiveness of antioxidants. Hum Reprod. 1998;13:1429-36.
11. Duru NK, Morshedi M, Oehninger S. Effects of hydrogen peroxide on DNA and plasma membrane integrity of human spermatozoa. Fertil Steril. 2000;74:1200-7.
12. Branco CS, Garcez ME, Pasqualotto FF, Erdtman B, Salvador M. Resveratrol and ascorbic acid prevent DNA damage induced by cryopreservation in human semen. Cryobiology. 2010;60:235-7.
13. Kalthur G, Raj S, Thiyagarajan A, Kumar S, Kumar P, Adiga SK. Vitamin E supplementation in semen-freezing medium improves the motility and protects sperm from freeze-thaw-induced DNA damage. Fertil Steril. 2011;95:1149-51.
14. Kaji M. Zinc in endocrinology. Pediatr Int. 2001;16:1-7.
15. Wong WY, Merkus HM, Thomas CM, Menkveld R, Zielthuis GA, Steegers-Theunissen RP. Effect of folic acid and zinc sulphate on male factor sub fertility, a double blind, randomized placed controlled trial. Fertil Steril. 2002;77:491-8.
16. Suzuki T, Nakajima K, Yamamoto A, Yamanaka H. Metallothionein binding zinc inhibits nuclear chromatin decondensation of human spermatozoa. Andrologia. 1995;27:161-4.
17. Bjorndahl L, Kvist U. A model for the importance of zinc in the dynamics of human sperm chromatin stabilization after ejaculation in relation to sperm DNA vulnerability. Syst Biol Reprod Med. 2011;57:86-92.
18. World Health Organization. Laboratory manual for the examination of human semen and sperm-cervical mucus interaction. Cambridge: Cambridge University Press; 1999.
19. Kalthur G, Adiga SK, Upadhya D, Rao S, Kumar P. Effect of cryopreservation on sperm DNA integrity in patients with teratospermia. Fertil Steril. 2008;89:1723-7.
20. Fernandez JL, Muriel L, Rivero MT, Goyanes V, Vazquez R, Alvarez JG. The sperm chromatin dispersion test: a simple method for the determination of sperm DNA fragmentation. J Androl. 2003;24:59-66.
21. Johnson LV, Walsh ML, Chen LB. Localization of mitochondria in living cells by rhodamine 123. Proc Natl Acad Sci USA. 1980;77:990-4.
22. Colas C, Grasa P, Casao A, Gallego M, Abecia JA, Forcada F. Changes in calmodulin immunocytochemical localization associated with capacitation and acrosomal exocytosis of ram spermatozoa. Theriogenology. 2009;71:789-800.
23. Garde JJ, Ortiz N, García A, Gallego L. Use of a triple-stain technique to detect viability and acrosome reaction in deer spermatozoa. Arch Androl. 1997;39:1-9.
24. Chohan KR, Griffin JT, Carrell DT. Evaluation of chromatin integrity in human sperm using acridine orange staining with different fixatives and after cryopreservation. Andrologia. 2004;36:321-6.
25. Adiga SK, Khan Z, Upadhya D, Kalthur G, Kumar P. Ability of deoxyribonucleic acid-damaged sperm to withstand freeze-thaw-induced damage during cryopreservation. Fertil Steril. 2009;92:959-63.
26. Erenpreiss J, Elzanaty S, Giwercman A. Sperm DNA damage in men from infertile couples. Asian J Androl. 2008;10:786-90.
27. Hammadeh ME, Askari AS, Georg T, Rosenbaum P, Schmidt W. Effect of freeze-thawing procedure on chromatin stability, morphological alteration and membrane integrity of human spermatozoa in fertile and subfertile men. Int J Androl. 1999;22:155-62.
28. Varghese AC, Bragais FM, Mukhopadhyay D, Kundu S, Pal M, Bhattacharyya AK. Human sperm DNA integrity in normal and abnormal semen samples and its correlation with sperm characteristics. Andrologia. 2009;41:207-15.
29. Berg JM. Zinc fingers and other metal-binding domains. Elements for interactions between macromolecules. J Biol Chem. 1990;265:6513-6.
30. Balhorn R. The protamine family of sperm nuclear proteins. Genome Biol. 2007;8:227.
31. Kvist U, Bjorndahl L. Zinc preserves an inherent capacity for human sperm chromatin decondensation. Acta Physiol Scand. 1985;24:195-200.
32. Danscher G, Hammen R, Fjerdingstad E, Rebbe H. Zinc content of human ejaculate and the motility of sperm cells. Int J Androl 1978;1:1576-81.
33. Riffo M, Leiva S, Astudillo J. Effect of zinc on human sperm motility and the acrosome reaction. Int J Androl. 1992;15:229-37.
34. Stankovic H, Mikac-Devic D. Zinc and copper in human semen. Clin Chem Acta. 1976;70:123-6.
35. Caldamone AA, Freytag MK, Cockett AT. Seminal zinc and male infertility. Urology. 1979;13:280-1.
36. Calvin HI, Hwang FH-F, Wohlrab H. Localisation of zinc in a dense fiber-connecting piece fraction of rat sperm tails analogous chemically to hair keratin. Biol Reprod. 1975;13:228-39.
37. Bettger WJ, O'Dell BL. A critical physiological role of zinc in the structure and function of biomembranes. Life Sci. 1981;28:1425-38.
38. Kendall NR, McMullen S, Green A, Rodway RG. The effect of a zinc, cobalt and selenium soluble glass bolus on trace element status and semen quality of ram lambs. Anim Reprod Sci. 2000;62:277-83.
39. Andrews JC, Nolan JP, Hammerstedt RH, Bavister BD. Role of zinc during hamster sperm capacitation. Biol Reprod. 1994;51:1238-47.
40. Bilaspuri GS, Babbar BK. Effect of albumin and zinc on capacitation and acrosome reaction of buffalo spermatozoa. Indian J Anim Sci. 2007;77:688-92.
41. Liu DY, Sie BS, Liu ML, Agresta F, Baker HW. Relationship between seminal plasma zinc concentration and spermatozoa-zona pellucida binding and the ZP-induced acrosome reaction in sub fertile men. Asian J Androl. 2009;11:499-507.