Carnitine content in the follicular fluid and expression of the enzymes involved in beta oxidation in oocytes and cumulus cells

Journal of Assisted Reproduction and Genetics

Volumn 29, Issue 11, 2012, Pages 1221-1225

  Montjean, Debbie ^a   Entezami, Frida ^b   Lichtblau, Isabelle ^a   Belloc, Stephanie ^a   Gurgan, Timur ^c   Menezo, Yves ^b,d  

^a Unilabs   (France)

^b Laboratoire Dynabio Polyclinique du Cotentin   (France)

^c The Gurgan clinic   (Turkey)

^d London Fertility Associates   (United Kingdom)

Author keywords

Carnitine; Human follicular fluid; Lipid betaoxidation; Mitochondria; Oocyte

Indexed keywords

CARNITINE; ESTRADIOL; GAMMA BUTYROBETAINE DIOXYGENASE; TRIMETHYL LYSINE HYDROXYLASE; UNCLASSIFIED DRUG;

ADULT; ARTICLE; CONTROLLED STUDY; CUMULUS CELL; ENZYME SYNTHESIS; ESTRADIOL BLOOD LEVEL; FATTY ACID OXIDATION; FEMALE; FERTILIZATION IN VITRO; HUMAN; HUMAN EMBRYO; MAJOR CLINICAL STUDY; OOCYTE; OOCYTE RETRIEVAL; OUTCOME ASSESSMENT; OVARY FOLLICLE FLUID; PREGNANT WOMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RANDOMIZED CONTROLLED TRIAL;

ADOLESCENT; ADULT; CARNITINE; CUMULUS CELLS; FEMALE; FERTILIZATION IN VITRO; FOLLICULAR FLUID; GAMMA-BUTYROBETAINE DIOXYGENASE; HUMANS; LIPID METABOLISM; MIXED FUNCTION OXYGENASES; OOCYTES; OXIDATION-REDUCTION; PREGNANCY; YOUNG ADULT;

EID: 84878353699     PISSN: 10580468     EISSN: 15737330     Source Type: Journal    
DOI: 10.1007/s10815-012-9855-2     Document Type: Article

References (22)

1. Oey NA, den Boer ME, Wijburg FA, Vekemans M, Augé J, Steiner C, Wanders RJ, Waterham HR, Ruiter JP, Attié-Bitach T. Long-chain fatty acid oxidation during early human development. Pediatr Res. 2005;57:755-9.
2. Cummins JM. The role of maternal mitochondria during oogenesis, fertilization and embryogenesis. Reprod Biomed Online. 2002;4:176-82.
3. Van Blerkom J. Mitochondrial function in the human oocyte and embryo and their role in developmental competence. Mitochondrion. 2011;11:797-813.
4. Van Blerkom J, Davis PW, Lee J. ATP content of human oocytes and developmental potential and outcome after in vitro fertilization and embryo transfer. Hum Reprod. 1995;10:415-24.
5. Downs SM, Mosey JL, Klinger J. Fatty acid oxidation and meiotic resumption in mouse oocytes. Mol Reprod Dev. 2009;76:844-53.
6. Dunning KR, Cashman K, Russell DL, Thompson JG, Norman RJ, Robker RL. Beta-oxidation is essential for mouse oocyte developmental competence and early embryo development. Biol Reprod. 2010;83:909-18.
7. Dunning KR, Akison LK, Russell DL, Norman RJ, Robker RL. Increased beta-oxidation and improved oocyte developmental competence in response to l-carnitine during ovarian in vitro follicle development in mice. Biol Reprod. 2011;85:548-55.
8. Hewitson LC, Martin KL, Leese HJ. Effects of metabolic inhibitors on mouse preimplantation embryo development and the energy metabolism of isolated inner cell masses. Mol Reprod Dev. 1996;43:323-30.
9. Ferguson EM, Leese HJ. A potential role for triglyceride as an energy source during bovine oocyte maturation and early embryo development. Mol Reprod Dev. 2006;73:1195-201.
10. Ménézo Y, Testart J, Thebault A, Frydman R, Khatchadourian C. The preovulatory follicular fluid in the human: influence of hormonal pretreatment (clomophene-hCG) on some biochemical and biophysical variables. Int J Fertil. 1982;27:47-51.
11. Menezo Jr Y, Russo G, Tosti E, El Mouatassim S, Benkhalifa M. Expression profile of genes coding for DNA repair in human oocytes using pangenomic microarrays, with a special focus on ROS linked decays. J Assist Reprod Genet. 2007;24:513-20.
12. Benkhalifa M, Montjean D, Cohen-Bacrie P, Ménézo Y. Imprinting: RNA expression for homocysteine recycling in the human oocyte. Fertil Steril. 2010;93:1585-90.
13. Ménézo Y, Pluntz L, Chouteau J, Gurgan T, Demirol A, Dalleac A, Benkhalifa M. Zinc concentrations in serum and follicular fluid during ovarian stimulation and expression of Zn2+ transporters in human oocytes and cumulus cells. Reprod Biomed Online. 2011;22:647-52.
14. Fenkci SM, Fenkci V, Oztekin O, Rota S, Karagenc N. Serum total L-carnitine levels in nonobese women with polycystic ovary syndrome. Hum Reprod. 2008;23:1602-6.
15. Takiyama N. Matsumoto K age-and sex-related differences of serum carnitine in a Japanese population. J Am Coll Nutr. 1998;17:71-4.
16. Jungheim ES, Macones GA, Odem RR, Patterson BW, Lanzendorf SE, Ratts VS, et al. Associations between free fatty acids, cumulus oocyte complex morphology and ovarian function during in vitro fertilization. Fertil Steril. 2011;95:1970-4.
17. Menezo Y, Testart J, Khatchadourian C, Frydman R. Human preovulatory follicular fluid: the lipids. Are they the trigger for capacitation? Int J Fertil. 1984;29:61-4.
18. Acton BM, Jurisicova A, Jurisica I, Casper RF. Alterations in mitochondrial membrane potential during preimplantation stages of mouse and human embryo development. Mol Hum Reprod. 2004;10:23-32.
19. Sutton-McDowall ML, Feil D, Robker RL, Thompson JG, Dunning KR. Utilization of endogenous fatty acid stores for energy production in bovine preimplantation embryos. Theriogenology. 2012;77:1632-41.
20. Hillman N, Flynn TJ. The metabolism of exogenous fatty acids by preimplantation mouse embryos developing in vitro. J Embryol Exp Morphol. 1980;56:157-68.
21. Wilding M, Dale B, Marino M, di Matteo L, Alviggi C, Pisaturo ML, Lombardi L, de Placido G. Mitochondrial aggregation patterns and activity in human oocytes and preimplantation embryos. Hum Reprod. 2001;16:909-17.
22. Abdelrazik H, Sharma R, Mahfouz R, Agarwal A. L-Carnitine decreases DNA damage and improves the in vitro blastocyst development rate in mouse embryos. Fertil Steril. 2009;91:589-96.