Questions about the accuracy of polar body analysis for preimplantation genetic screening

Human Reproduction

Volumn 28, Issue 6, 2013, Pages 1731-1732

  Fragouli, Elpida ^a,b   Wells, Dagan ^a,b  

^a UNIVERSITY OF OXFORD   (United Kingdom)

^b Reprogenetics LLC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANEUPLOIDY; BLASTOCYST; BLASTOMA; CHROMOSOME MOSAICISM; COMPARATIVE GENOMIC HYBRIDIZATION; DISOMY; GENETIC SCREENING; HOMOZYGOSITY; HUMAN; INNER CELL MASS; KARYOTYPE; LETTER; POLAR BODY; PRENATAL DIAGNOSIS; TRISOMY; UNIPARENTAL DISOMY; CHROMOSOME SEGREGATION; CYTOLOGY; EMBRYO DEVELOPMENT; FEMALE; GENETICS; MEIOSIS; PHYSIOLOGY; PROCEDURES; TROPHOBLAST;

BLASTOMERES; CHROMOSOME SEGREGATION; EMBRYONIC DEVELOPMENT; FEMALE; HUMANS; MEIOSIS; POLAR BODIES; PREIMPLANTATION DIAGNOSIS; TROPHOBLASTS;

EID: 84878330681     PISSN: 02681161     EISSN: 14602350     Source Type: Journal    
DOI: 10.1093/humrep/det069     Document Type: Letter

References (8)

1. Capalbo A, Bono S, Spizzichino L, Biricik A, Baldi M, Colamaria S, Ubaldi FM, Rienzi L, Fiorentino F. Sequential comprehensive chromosome analysis on polar bodies, blastomeres and trophoblast: insights into female meiotic errors and chromosomal segregation in the preimplantation window of embryo development. Hum Reprod 2013;28:509-518.
2. Fragouli E, Alfarawati S, Daphnis DD, Goodall NN, Mania A, Griffiths T, Gordon A, Wells D. Cytogenetic analysis of human blastocysts with the use of FISH, CGH and aCGH: scientific data and technical evaluation. Hum Reprod 2011a;26:480-490.
3. Fragouli E, Alfarawati S, Goodall NN, Sánchez-García JF, Colls P, Wells D. The cytogenetics of polar bodies: insights into female meiosis and the diagnosis of aneuploidy. Mol Hum Reprod 2011b;17:286-295.
4. Gabriel AS, Thornhill AR, Ottolini CS, Gordon A, Brown AP, Taylor J, Bennett K, Handyside A, Griffin DK. Array comparative genomic hybridisation on first polar bodies suggests that non-disjunction is not the predominant mechanism leading to aneuploidy in humans. J Med Genet 2011;48:433-437.
5. Handyside AH, Montag M, Magli MC, Repping S, Harper J, Schmutzler A, Vesela K, Gianaroli L, Geraedts J. Multiple meiotic errors caused by predivision of chromatids in women of advanced maternal age undergoing in vitro fertilisation. Eur J Hum Genet 2012;20: 742-747.
6. Magli MC, Montag M, Köster M, Muzi L, Geraedts J, Collins J, Goossens V, Handyside AH, Harper J, Repping S et al. Polar body array CGH for prediction of the status of the corresponding oocyte. Part II: technical aspects. Hum Reprod 2011;26:3181-3185.
7. Northrop LE, Treff NR, Levy B, Scott RT. SNP microarray based 24 chromosome aneuploidy screening demonstrates that cleavage stage FISH poorly predicts aneuploidy in embryos that develop to morphologically normal blastocysts. Mol Hum Reprod 2010; 16:590-600.
8. Wells D, Alfarawati S, Konstantinidis M, Jaroudi S, Fragouli E. Characterisation of mechanisms causing oocyte aneuploidy reveals a flaw in embryo selection strategies based upon polar body chromosome screening. Hum Reprod 2012;27:ii106-ii108.