Monitoring of protease catalyzed reactions by quantitative MALDI MS using metal labeling

Analytical Chemistry

Volumn 85, Issue 10, 2013, Pages 5184-5190

  Gregorius, Barbara ^a   Jakoby, Thomas ^a   Schaumlöffel, Dirk ^b   Tholey, Andreas ^a  

^a UNIVERSITY OF KIEL   (Germany)

^b UNIVERSITÉ DE PAU ET DES PAYS DE L'ADOUR   (France)

Author keywords

[No Author keywords available]

Indexed keywords

BIOTECHNOLOGICAL APPLICATIONS; CATALYZED REACTIONS; COMPLEX SUBSTRATES; LABELED INTERNAL STANDARDS; ORDERS OF MAGNITUDE; PROTEOLYTIC REACTION; QUANTITATIVE MASS SPECTROMETRIES; RARE EARTH METAL IONS;

ENZYME ACTIVITY; ISOTOPES; MASS SPECTROMETRY; METAL IONS; MULTIPLEXING; PEPTIDES;

SUBSTRATES;

METAL; NEUROTENSIN; TRYPSIN;

AMINO ACID SEQUENCE; ARTICLE; BIOCATALYSIS; CHEMISTRY; ENZYME ASSAY; KINETICS; MASS SPECTROMETRY; METABOLISM; METHODOLOGY; PROTEIN DEGRADATION; STAINING;

AMINO ACID SEQUENCE; BIOCATALYSIS; ENZYME ASSAYS; KINETICS; METALS; NEUROTENSIN; PROTEOLYSIS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; STAINING AND LABELING; TRYPSIN;

EID: 84878323797     PISSN: 00032700     EISSN: 15206882     Source Type: Journal    
DOI: 10.1021/ac4005452     Document Type: Article

References (25)

1. Wallenfels, K.; Weil, R. The Enzymes; Academic Press: New York, 1972; Vol. VII, p 617.
2. Liesener, A.; Karst, U. Anal. Bioanal. Chem. 2005, 382, 1451-64
3. Xia, Z.; Xing, Y.; So, M. K.; Koh, A. L.; Sinclair, R.; Rao, J. Anal. Chem. 2008, 80, 8649-55
4. Gerber, S. A.; Scott, C. R.; Turecek, F.; Gelb, M. H. Anal. Chem. 2001, 73, 1651-7
5. Norris, A. J.; Whitelegge, J. P.; Faull, K. F.; Toyokuni, T. Biochemistry 2001, 40, 3774-9
6. Konermann, L.; Douglas, D. J. Methods Enzymol. 2002, 354, 50-64
7. Liesener, A.; Karst, U. Analyst 2005, 130, 850-4
8. Bothner, B.; Chavez, R.; Wei, J.; Strupp, C.; Phung, Q.; Schneemann, A.; Siuzdak, G. J. Biol. Chem. 2000, 275, 13455-9
9. Schluter, H.; Jankowski, J.; Rykl, J.; Thiemann, J.; Belgardt, S.; Zidek, W.; Wittmann, B.; Pohl, T. Anal. Bioanal. Chem. 2003, 377, 1102-7
10. Bungert, D.; Heinzle, E.; Tholey, A. Anal. Biochem. 2004, 326, 167-75
11. Tholey, A.; Zabet-Moghaddam, M.; Heinzle, E. Anal. Chem. 2006, 78, 291-7
12. Gusev, A. I.; Wilkinson, W. R.; Proctor, A.; Hercules, D. M. Anal. Bioanal. Chem. 1996, 354, 455-63
13. Meng, Z.; Limbach, P. A. Anal. Chem. 2005, 77, 1891-5
14. Ross, P. L.; Huang, Y. N.; Marchese, J. N.; Williamson, B.; Parker, K.; Hattan, S.; Khainovski, N.; Pillai, S.; Dey, S.; Daniels, S.; Purkayastha, S.; Juhasz, P.; Martin, S.; Bartlet-Jones, M.; He, F.; Jacobson, A.; Pappin, D. J. Mol. Cell. Proteomics 2004, 3, 1154-69
15. Thompson, A.; Schafer, J.; Kuhn, K.; Kienle, S.; Schwarz, J.; Schmidt, G.; Neumann, T.; Johnstone, R.; Mohammed, A. K.; Hamon, C. Anal. Chem. 2003, 75, 1895-904
16. Whetstone, P. A.; Butlin, N. G.; Corneillie, T. M.; Meares, C. F. Bioconjugate Chem. 2004, 15, 3-6
17. Ahrends, R.; Pieper, S.; Kuhn, A.; Weisshoff, H.; Hamester, M.; Lindemann, T.; Scheler, C.; Lehmann, K.; Taubner, K.; Linscheid, M. W. Mol. Cell. Proteomics 2007, 6, 1907-16
18. Rappel, C.; Schaumlöffel, D. Anal. Chem. 2009, 81, 385-393
19. Gregorius, B.; Schaumlöffel, D.; Hildebrandt, A.; Tholey, A. Rapid Commun. Mass Spectrom. 2010, 24, 3279-89
20. Tholey, A.; Schaumlöffel, D. TrAC, Trends Anal. Chem. 2010, 29, 399-408
21. Lathia, U. S.; Ornatsky, O.; Baranov, V.; Nitz, M. Anal. Biochem. 2010, 398, 93-8
22. Lathia, U. S.; Ornatsky, O.; Baranov, V.; Nitz, M. Anal. Biochem. 2011, 408, 157-9
23. Kutscher, D. J.; del Castillo Busto, M. E.; Zinn, N.; Sanz-Medel, A.; Bettmer, J. J. Anal. At. Spectrom. 2008, 23, 1359-1364
24. Gregorius, B.; Jakoby, T.; Schaumlöffel, D.; Tholey, A. Anal. Bioanal. Chem. 2013, 405, 2735-41
25. Schilling, O.; Overall, C. M. Nat. Biotechnol. 2008, 26, 685-94