Introduction to spin label electron paramagnetic resonance spectroscopy of proteins

Biochemistry and Molecular Biology Education

Volumn 41, Issue 3, 2013, Pages 156-162

  Melanson, Michelle ^a   Sood, Abha ^a   Török, Fanni ^a   Török, Marianna ^a  

^a UNIVERSITY OF MASSACHUSETTS BOSTON   (United States)

Author keywords

5 MSL; Cystein labeling; EPR; ESR; Gel filtration chromatography; Hemoglobin; Magnetic resonance; MTSL; Nitroxide; Protein Data Bank; PyMOL; Spin label; Undergraduate

Indexed keywords

3 MALEIMIDO 2,2,5,5 TETRAMETHYLPYRROLIDINE 1 OXYL; AMINE OXIDE; HEMOGLOBIN; N-(1-OXYL-2,2,5,5-TETRAMETHYL-3-PYRROLIDINYL)MALEIMIDE;

ARTICLE; BINDING SITE; CHEMICAL STRUCTURE; CHEMISTRY; CURRICULUM; ELECTRON SPIN RESONANCE; HUMAN; METHODOLOGY; PROTEIN BINDING; PROTEIN CONFORMATION; SPIN LABELING; STUDENT;

BINDING SITES; CURRICULUM; CYCLIC N-OXIDES; ELECTRON SPIN RESONANCE SPECTROSCOPY; HEMOGLOBINS; HUMANS; MODELS, MOLECULAR; PROTEIN BINDING; PROTEIN CONFORMATION; SPIN LABELS; STUDENTS;

EID: 84878174554     PISSN: 14708175     EISSN: 15393429     Source Type: Journal    
DOI: 10.1002/bmb.20677     Document Type: Article

References (19)

1. What is EPR? Bruker BioSpin Home Page. http://www.bruker-biospin.com/whatisepr.html (accessed July 2012).
2. D. Sheehan (2000) Physical Biochemistry: Principles and Applications, Wiley, New York, pp. 111-113.
3. E. K. Zavoisky (1945) Spin-magnetic resonance in paramagnetics, J. Phys. USSR 9, 211-245.
4. H. Beinert (1985) Electron paramagnetic resonance spectroscopy in Biochemistry: Past, present, future, Biochem. Soc. Trans. 13, 541-547.
5. V. Levi, F. Flecha (2003) Labeling of proteins with fluorescent probes: Photophysical characterization of dansylated bovine serum albumin, Biochem. Mol. Biol. Ed. 31, 333-336.
6. P. G. Fajer, in R. A. Meyers, Ed. (2000) Electron spin resonance spectroscopy labeling in peptide and protein analysis, Encyclopedia of Analytical Chemistry, Wiley, pp. 1-33.
7. T. J. Stone, T. Buckman, P. L. Nordio, H. M. McConnell (1965) Spin-labeled Biomolecules, Proc. Natl. Acad. Sci. USA 54, 1010-1017.
8. D. Marsh (2010) Electron spin resonance in membrane research: protein-lipid interactions from challenging beginnings to state of the art, Eur. Biophys. J. Biophy. 39, 513-525.
9. W. L. Hubbell, A. Gross, R. Langen, M. A. Lietzow (1998) Recent advances in site-directed spin labeling of proteins, Curr. Opin. Struct. Biol. 8, 649-656.
10. G. E. Fanucci, D. S. Cafiso (2006) Recent advances and applications in site-directed spin labeling, Curr. Opin. Struct. Biol. 16, 1-10.
11. RCSB Protein Data Bank. http://www.pdb.org (accessed July 2012).
12. PyMOL http://pymol.org/ (accessed July 2012)
13. PD-10 Desalting Columns Instruction/Protocol, GE Healthcare Life Sciences, http://www.gelifesciences.com/ (accessed September 2012).
14. J. S. Hyde, in C. H. W. Hirs, S. N. Timasheff, Eds. (1978) Saturation transfer spectroscopy, Methods in Enzymology, Academic Press, New York, 49, pp. 480-511.
15. L. I. Horvath, M. Török, K. Hideg, L. Dux (1997) Dynamic aspects of the incorporation of proteins into biological membranes, J. Mol. Recognit., 10, 188-193.
16. M. Margittai, R. Langen, in I. Kheterpal, R. Wetzel, Eds. (2006)Spin labeling analysis of amyloids and other protein aggregates, Methods in Enzymology, Academic Press, New York, 413, pp. 122-139.
17. M. Török, S. Milton, R. Kayed, T. McIntire, C. G. Glabe, R. Langen (2002) Structural and dynamic features of Alzheimer's Aβ peptide in amyloid fibrils studied by site-directed spin labeling, J. Biol. Chem., 277, 40810-40815.
18. J. M. Isas, R. Langen, H. T. Haigler, W. L. Hubbell (2002) Structure and dynamics of a helical hairpin and loop region in annexin 12: A site-directed spin labeling study, Biochemistry, 41, 1464-1473.
19. D. Voet, J. G. Voet, C. W. Pratt (2008) Fundamentals of Biochemistry, 3rd. ed., Wiley, New York, pp. 176-197.