Wicking: A Rapid Method for Manually Inserting Ion Channels into Planar Lipid Bilayers

PLoS ONE

Volumn 8, Issue 5, 2013, Pages

  Costa, Justin A ^a   Nguyen, Dac A ^a,c   Leal Pinto, Edgar ^b   Gordon, Ronald E ^a   Hanss, Basil ^a  

^a MOUNT SINAI SCHOOL OF MEDICINE   (United States)

^b VIRGINIA COMMONWEALTH UNIVERSITY   (United States)

^c NATIONAL INSTITUTES OF HEALTH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA HEMOLYSIN; CHLORIDE CHANNEL; ION CHANNEL; PROTEIN CLIC1; UNCLASSIFIED DRUG;

ARTICLE; CHEMICAL MODIFICATION; CHEMICAL PROCEDURES; CONTROLLED STUDY; ION CONDUCTANCE; LIPID BILAYER; PHYSICAL CHEMISTRY; PROCESS DEVELOPMENT; WICKING TECHNIQUE;

ALGORITHMS; BACTERIAL PROTEINS; CAPILLARY ACTION; CHLORIDE CHANNELS; ELECTRIC CAPACITANCE; GLYCOLATES; HEK293 CELLS; HEMOLYSIN PROTEINS; HUMANS; ION CHANNEL GATING; ION CHANNELS; LIPID BILAYERS; LIPOSOMES; MEMBRANE POTENTIALS; PHOSPHATIDYLETHANOLAMINES; PHOSPHATIDYLSERINES;

EID: 84878161542     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0060836     Document Type: Article

References (36)

1. Bean RC, Shepherd WC, Chan H, Eichner J, (1969) Discrete conductance fluctuations in lipid bilayer protein membranes. The Journal of general physiology 53: 741-757.
2. Hladky SB, Haydon DA, (1970) Discreteness of conductance change in bimolecular lipid membranes in the presence of certain antibiotics. Nature 225: 451-453.
3. Antonov VF, Petrov VV, Molnar AA, Predvoditelev DA, Ivanov AS, (1980) The appearance of single-ion channels in unmodified lipid bilayer membranes at the phase transition temperature. Nature 283: 585-586.
4. Fujii G, Chang JE, Coley T, Steere B, (1997) The formation of amphotericin B ion channels in lipid bilayers. Biochemistry 36: 4959-4968.
5. Feigin AM, Takemoto JY, Wangspa R, Teeter JH, Brand JG, (1996) Properties of voltage-gated ion channels formed by syringomycin E in planar lipid bilayers. The Journal of membrane biology 149: 41-47.
6. Mueller P, Rudin DO, Tien HT, Wescott WC, (1962) Reconstitution of cell membrane structure in vitro and its transformation into an excitable system. Nature 194: 979-980.
7. Woodbury DJ, Miller C, (1990) Nystatin-induced liposome fusion. A versatile approach to ion channel reconstitution into planar bilayers. Biophysical journal 58: 833-839.
8. Tien HT (2000) Membrane Biophysics as viewed from experimental bilayer lipid membranes; Ottova-Leitmanova A, editor. Amsterdam, The Netherlands: Elsevier.
9. Funakoshi K, Suzuki H, Takeuchi S, (2006) Lipid bilayer formation by contacting monolayers in a microfluidic device for membrane protein analysis. Analytical chemistry 78: 8169-8174.
10. Malmstadt N, Nash MA, Purnell RF, Schmidt JJ, (2006) Automated formation of lipid-bilayer membranes in a microfluidic device. Nano letters 6: 1961-1965.
11. Poulos JL, Jeon TJ, Damoiseaux R, Gillespie EJ, Bradley KA, et al. (2009) Ion channel and toxin measurement using a high throughput lipid membrane platform. Biosensors & bioelectronics 24: 1806-1810.
12. Simonsson L, Gunnarsson A, Wallin P, Jonsson P, Hook F, (2011) Continuous lipid bilayers derived from cell membranes for spatial molecular manipulation. Journal of the American Chemical Society 133: 14027-14032.
13. Hasan N, Corbin D, Hu C, (2010) Fusogenic pairings of vesicle-associated membrane proteins (VAMPs) and plasma membrane t-SNAREs - VAMP5 as the exception. PloS one 5: e14238.
14. White SH, Chang W, (1981) Voltage dependence of the capacitance and area of black lipid membranes. Biophysical journal 36: 449-453.
15. Toyama S, Nakamura A, Toda F, (1991) Measurement of voltage dependence of capacitance of planar bilayer lipid membrane with a patch clamp amplifier. Biophysical journal 59(4): 934-944.
16. Hille B (2001) Ion channels of excitable membranes. Sunderland, MA: Sinauer Associates, Inc.
17. Warton K, Tonini R, Fairlie WD, Matthews JM, Valenzuela SM, et al. (2002) Recombinant CLIC1 (NCC27) assembles in lipid bilayers via a pH-dependent two-state process to form chloride ion channels with identical characteristics to those observed in Chinese hamster ovary cells expressing CLIC1. The Journal of biological chemistry 277: 26003-26011.
18. Tulk BM, Schlesinger PH, Kapadia SA, Edwards JC, (2000) CLIC-1 functions as a chloride channel when expressed and purified from bacteria. The Journal of biological chemistry 275: 26986-26993.
19. Valenzuela SM, Martin DK, Por SB, Robbins JM, Warton K, et al. (1997) Molecular cloning and expression of a chloride ion channel of cell nuclei. The Journal of biological chemistry 272: 12575-12582.
20. Prevost G, Mourey L, Colin DA, Menestrina G, (2001) Staphylococcal pore-forming toxins. Current topics in microbiology and immunology 257: 53-83.
21. Bakas L, Ostolaza H, Vaz WL, Goni FM, (1996) Reversible adsorption and nonreversible insertion of Escherichia coli alpha-hemolysin into lipid bilayers. Biophysical journal 71: 1869-1876.
22. Le Pioufle B, Suzuki H, Tabata KV, Noji H, Takeuchi S, (2008) Lipid bilayer microarray for parallel recording of transmembrane ion currents. Analytical chemistry 80: 328-332.
23. Mach T, Chimerel C, Fritz J, Fertig N, Winterhalter M, et al. (2008) Miniaturized planar lipid bilayer: increased stability, low electric noise and fast fluid perfusion. Analytical and bioanalytical chemistry 390: 841-846.
24. Maglia G, Restrepo MR, Mikhailova E, Bayley H, (2008) Enhanced translocation of single DNA molecules through alpha-hemolysin nanopores by manipulation of internal charge. Proceedings of the National Academy of Sciences of the United States of America 105: 19720-19725.
25. Mathe J, Aksimentiev A, Nelson DR, Schulten K, Meller A, (2005) Orientation discrimination of single-stranded DNA inside the alpha-hemolysin membrane channel. Proceedings of the National Academy of Sciences of the United States of America 102: 12377-12382.
26. McGillivray DJ, Valincius G, Heinrich F, Robertson JW, Vanderah DJ, et al. (2009) Structure of functional Staphylococcus aureus alpha-hemolysin channels in tethered bilayer lipid membranes. Biophysical journal 96: 1547-1553.
27. Oukhaled G, Mathe J, Biance AL, Bacri L, Betton JM, et al. (2007) Unfolding of proteins and long transient conformations detected by single nanopore recording. Physical review letters 98: 158101.
28. Pastoriza-Gallego M, Oukhaled G, Mathe J, Thiebot B, Betton JM, et al. (2007) Urea denaturation of alpha-hemolysin pore inserted in planar lipid bilayer detected by single nanopore recording: loss of structural asymmetry. FEBS letters 581: 3371-3376.
29. Roskamp RF, Vockenroth IK, Eisenmenger N, Braunagel J, Koper I, (2008) Functional tethered bilayer lipid membranes on aluminum oxide. Chemphyschem: a European journal of chemical physics and physical chemistry 9: 1920-1924.
30. Suzuki H, Tabata KV, Noji H, Takeuchi S, (2007) Electrophysiological recordings of single ion channels in planar lipid bilayers using a polymethyl methacrylate microfluidic chip. Biosensors & bioelectronics 22: 1111-1115.
31. Szoszkiewicz R, Ainavarapu SR, Wiita AP, Perez-Jimenez R, Sanchez-Ruiz JM, et al. (2008) Dwell time analysis of a single-molecule mechanochemical reaction. Langmuir: the ACS journal of surfaces and colloids 24: 1356-1364.
32. McNally B, Wanunu M, Meller A, (2008) Electromechanical unzipping of individual DNA molecules using synthetic sub-2 nm pores. Nano letters 8: 3418-3422.
33. Goodchild SC AC, Breit SN, Curmi PM, Brown LJ, (2011) Transmembrane extension and oligomerization of the CLIC1 chloride intracellular channel protein upon membrane interaction. Biochemistry 50: 10887-10897.
34. Harrop SJ, DeMaere MZ, Fairlie WD, Reztsova T, Valenzuela SM, Mazzanti M, et al. (2001) Crystal Structure of the Soluble Form of the Intracellular Chloride Channel NCC27 (CLIC1) at 1.4Å Resolution. Journal of Biological Chemistry 276: 44993-45000.
35. Goodchild SC Howell MW, Littler DR, Mandyam RA, Sale KL, Mazzanti M, et al. (2010) Metamorphic response of the CLIC1 chloride intracellular ion channel protein upon membrane interaction. Biochemistry 49: 5278-5289.
36. Vockenroth IK, Atanasova PP, Jenkins AT, Koper I, (2008) Incorporation of alpha-hemolysin in different tethered bilayer lipid membrane architectures. Langmuir: the ACS journal of surfaces and colloids 24: 496-502.