Performance of flow cytometry to screen urine for bacteria and white blood cells prior to urine culture

Clinical Biochemistry

Volumn 46, Issue 9, 2013, Pages 810-813

  Giesen, Callen D ^a   Greeno, Amanda M ^a   Thompson, Katherine A ^a   Patel, Robin ^a   Jenkins, Sarah M ^b   Lieske, John C ^a  

^a MAYO CLINIC ROCHESTER   (United States)

^b MAYO CLINIC   (United States)

Author keywords

Flow cytometry; Gram stain; Sysmex UF 1000i; Urinary tract infection; Urine culture

Indexed keywords

ARTICLE; BACTERIAL COUNT; BACTERIAL GROWTH; BACTERIUM CULTURE; ERYTHROCYTE COUNT; FALSE POSITIVE RESULT; FLOW CYTOMETRY; GRAM STAINING; HUMAN; LEUKOCYTE COUNT; PREDICTIVE VALUE; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; URINARY TRACT INFECTION; URINE CULTURE;

AREA UNDER CURVE; BACTERIURIA; CULTURE TECHNIQUES; FALSE POSITIVE REACTIONS; FEMALE; FLOW CYTOMETRY; GRAM-POSITIVE BACTERIA; HUMANS; LEUKOCYTE COUNT; MALE; ROC CURVE; URINALYSIS;

EID: 84877925480     PISSN: 00099120     EISSN: 18732933     Source Type: Journal    
DOI: 10.1016/j.clinbiochem.2013.03.005     Document Type: Article

References (20)

1. Foxman B. Epidemiology of urinary tract infections: incidence, morbidity, and economic costs. Am J Med 2002, 113(Suppl. 1A):5S-13S.
2. Schmiemann G., Kniehl E., Gebhardt K., Matejczyk M.M., Hummers-Pradier E. The diagnosis of urinary tract infection: a systematic review. Dtsch Arztebl Int 2010, 107:361-367.
3. Stamm W.E., Hooton T.M. Management of urinary tract infections in adults. N Engl J Med 1993, 329:1328-1334.
4. Johnson J.R., Stamm W.E. Urinary tract infections in women: diagnosis and treatment. Ann Intern Med 1989, 111:906-917.
5. Gupta K., Hooton T.M., Stamm W.E. Increasing antimicrobial resistance and the management of uncomplicated community-acquired urinary tract infections. Ann Intern Med 2001, 135:41-50.
6. Arslan S., Caksen H., Rastgeldi L., Uner A., Oner A.F., Odabas D. Use of urinary gram stain for detection of urinary tract infection in childhood. Yale J Biol Med 2002, 75:73-78.
7. Wiwanitkit V., Udomsantisuk N., Boonchalermvichian C. Diagnostic value and cost utility analysis for urine gram stain and urine microscopic examination as screening tests for urinary tract infection. Urol Res 2005, 33:220-222.
8. Broeren M.A., Bahceci S., Vader H.L., Arents N.L. Screening for urinary tract infection with the sysmex uf-1000i urine flow cytometer. J Clin Microbiol 2011, 49:1025-1029.
9. De Rosa R., Grosso S., Bruschetta G., Avolio M., Stano P., Modolo M.L., et al. Evaluation of the sysmex uf1000i flow cytometer for ruling out bacterial urinary tract infection. Clin Chim Acta 2010, 411:1137-1142.
10. Manoni F., Fornasiero L., Ercolin M., Tinello A., Ferrian M., Hoffer P., et al. Cutoff values for bacteria and leukocytes for urine flow cytometer sysmex uf-1000i in urinary tract infections. Diagn Microbiol Infect Dis 2009, 65:103-107.
11. Okada H., Horie S., Inoue S., Kawashima Y. The basic performance of bacteria counting for diagnosis of urinary tract infection using the fully automated urine particle analyzer uf-1000i. Sysmex Journal International 2007, 17:95-101.
12. Pieretti B., Brunati P., Pini B., Colzani C., Congedo P., Rocchi M., et al. Diagnosis of bacteriuria and leukocyturia by automated flow cytometry compared with urine culture. J Clin Microbiol 2010, 48:3990-3996.
13. van der Zwet W.C., Hessels J., Canbolat F., Deckers M.M. Evaluation of the sysmex uf-1000i(r) urine flow cytometer in the diagnostic work-up of suspected urinary tract infection in a Dutch general hospital. Clin Chem Lab Med 2010, 48:1765-1771.
14. Wang J., Zhang Y., Xu D., Shao W., Lu Y. Evaluation of the sysmex uf-1000i for the diagnosis of urinary tract infection. Am J Clin Pathol 2010, 133:577-582.
15. Hallin M., Deplano A., Roisin S., Boyart V., De Ryck R., Nonhoff C., et al. Pseudo-outbreak of extremely drug-resistant Pseudomonas aeruginosa urinary tract infections due to contamination of an automated urine analyzer. J Clin Microbiol 2012, 50:580-582.
16. Analyse-it for Microsoft excel 2009, Analyse-it Software, Ltd, Leeds, England, United Kingdom. 2.26.
17. Jmp 2010, SAS Institute Inc., Cary, NC. 9.
18. Naber K.G. Treatment options for acute uncomplicated cystitis in adults. J Antimicrob Chemother 2000, 46(Suppl. 1):23-27. [discussion 63-5].
19. Hummers-Pradier E., Koch M., Ohse A.M., Heizmann W.R., Kochen M.M. Antibiotic resistance of urinary pathogens in female general practice patients. Scand J Infect Dis 2005, 37:256-261.
20. Shaw K.N., McGowan K.L., Gorelick M.H., Schwartz J.S. Screening for urinary tract infection in infants in the emergency department: which test is best?. Pediatrics 1998, 101:E1.