Comparison of monomeric and polymeric horseradish peroxidase as labels in competitive ELISA for small molecule detection

Microchimica Acta

Volumn 180, Issue 7-8, 2013, Pages 711-717

  Li, Dongyang ^a,b   Ying, Yibin ^a   Wu, Jian ^a   Niessner, Reinhard ^b   Knopp, Dietmar ^b  

^a ZHEJIANG UNIVERSITY   (China)

^b TECHNICAL UNIVERSITY OF MUNICH   (Germany)

Author keywords

ELISA; PolyHRP; Signal amplification; Small molecule

Indexed keywords

EID: 84877796741     PISSN: 00263672     EISSN: 14365073     Source Type: Journal    
DOI: 10.1007/s00604-013-0974-y     Document Type: Article

References (17)

1. Price CP (1998) The evolution of immunoassay as seen through the journal clinical chemistry. Clin Chem 44(10): 2071-2074.
2. Avrameas S, Ternynck T (1971) Peroxidase labelled antibody and Fab conjugates with enhanced intracellular penetration. Immunochemistry 8(12): 1175-1179.
3. Wilson MB, Nakane PK (1978) Recent development in the periodate methods of conjugating horseradish peroxidase (HRPO) to antibodies. In: Knapp W, Holubar K, Wick G (eds) Immunofluorescence and related staining techniques. Elsevier/North Holland Biomedical Press, Amsterdam, pp 215-224.
4. Avrameas S, Ternynck T, Guesdon JL (1978) Coupling of enzymes to antibodies and antigens. Scand J Immunol 8: 7-23.
5. Vasilov RG, Tsitsikov EN (1990) An ultrasensitive immunoassay for human IgE measurement in cell-culture supernatant. Immunol Lett 26: 283-284.
6. Damen CWN, De Groot ER, Heij M, Boss DS, Schellens JHM, Rosing H, Beijnen JH, Aarden LA (2009) Development and validation of an enzyme-linked immunosorbent assay for the quantification of trastuzumab in human serum and plasma. Anal Biochem 391: 114-120.
7. Dhawan S (2002) Design and construction of novel molecular conjugates for signal amplification (I): conjugation of multiple horseradish peroxidase molecules to immunoglobulin via primary amines on lysine peptide chains. Peptides 23: 2091-2098.
8. Marquette CA, Hezard P, Degiuli A, Blum LJ (2006) Macro-molecular chemiluminescent complex for enhanced immuno-detection onto microtiter plate and protein biochip. Sensor Actuators 113: 664-670.
9. Charbgoo F, Mirshahi M, Sarikhani S, Abolhassan MF (2012) Synthesis of a unique high-performance poly-horseradish peroxidase complex to enhance sensitivity of immunodetection systems. Biotechnol Appl Biochem 59(1): 45-49.
10. Kensler TW, Roebuck BD, Wogan GN, Groopman JD (2011) Aflatoxin: a 50-year odyssey of mechanistic and translational toxicology. Toxicol Sci 120(suppl 1): S28-S48.
11. Cervino C, Weber E, Knopp D, Niessner R (2008) Comparison of hybridoma screening methods for the efficient detection of high-affinity hapten-specific monoclonal antibodies. J Immunol Methods 329(1-2): 184-193.
12. Haugland RP, You WW (2008) Coupling of antibodies with biotin. In: McMahon RJ (ed) Avidin-Biotin interactions. Humana Press, Totowa, pp 13-23.
13. Ahn KC, Lohstroh P, Gee SJ, Gee NA, Lasley B, Hammock BD (2007) High-throughput automated luminescent magnetic particle-based immunoassay to monitor human exposure to pyrethroid insecticides. Anal Chem 79(23): 8883-8890.
14. Tijssen P (1985) Practice and theory of enzyme immunoassays, vol 15. Elsevier Science, Amsterdam.
15. Harvey D (2000) Modern analytical chemistry. McGraw-Hill, Boston.
16. Ambrosi A, Airo F, Merkoci A (2010) Enhanced gold nanoparticle based ELISA for a breast cancer biomarker. Anal Chem 82(3): 1151-1156.
17. Mock DM, Bogusiewicz A (2008) Biotin-protein bond: Instability and structural modification to provide stability for in vivo applications. In: McMahon RJ (ed) Avidin-Biotin interactions. Humana Press, Totowa, pp 209-220.