The biochemical and immunological characterization of two serpins from Clonorchis sinensis

Molecular Biology Reports

Volumn 40, Issue 6, 2013, Pages 3977-3985

  Lei, Huali ^a,b   Tian, Yanli ^a,b   Chen, Wenjun ^a,b   Wang, Xiaoyun ^a,b   Li, Xuerong ^a,b   Mao, Qiang ^a,b   Sun, Jiufeng ^a,b   Li, Ran ^a,b   Xu, Yanquan ^a,b   Liang, Chi ^a,b   Huang, Yan ^a,b   Yu, Xinbing ^a,b  

^a SUN YAT SEN UNIVERSITY   (China)

^b SUN YAT SEN UNIVERSITY   (China)

Author keywords

Clonorchis sinensis; Cytokine; Immunoregulation; Serine proteases; Serpin

Indexed keywords

CHYMOTRYPSIN; CYSTEINE PROTEINASE; GAMMA INTERFERON; IMMUNOGLOBULIN G1 ANTIBODY; IMMUNOGLOBULIN G2A ANTIBODY; INTERLEUKIN 4; RECOMBINANT PROTEIN; SERINE; SERINE PROTEINASE INHIBITOR; SERPIN 2; THROMBIN; TRYPSIN; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANTIBODY BLOOD LEVEL; ARTICLE; CLONORCHIASIS; CLONORCHIS SINENSIS; CONTROLLED STUDY; CYTOKINE PRODUCTION; CYTOKINE RELEASE; IMMUNOLOCALIZATION; IMMUNOREGULATION; MALE; METACERCARIA; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; RAT; SEQUENCE ANALYSIS; SERPIN GENE; SERPIN2 GENE; THERMOSTABILITY; WESTERN BLOTTING;

AMINO ACID SEQUENCE; ANIMALS; BLOTTING, WESTERN; CLONORCHIS SINENSIS; CYTOKINES; GENE EXPRESSION REGULATION, DEVELOPMENTAL; IMMUNIZATION; IMMUNOGLOBULIN G; LIFE CYCLE STAGES; MALE; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; PROTEIN STABILITY; RABBITS; RATS; RATS, SPRAGUE-DAWLEY; RECOMBINANT PROTEINS; RNA, MESSENGER; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, PROTEIN; SERPINS; SPLEEN;

CLONORCHIS SINENSIS; ORYCTOLAGUS CUNICULUS; RATTUS; VERMES;

EID: 84877779831     PISSN: 03014851     EISSN: 15734978     Source Type: Journal    
DOI: 10.1007/s11033-012-2475-1     Document Type: Article

References (43)

1. Rim HJ (2005) Clonorchiasis: an update. J Helminthol 79(3):269-281
2. Liao WC, Wang HP, Chiu HM, Chang CY, Lin JT (2006) Multiple hepatic nodules: rare manifestation of clonorchiasis. J Gastroenterol Hepatol 21(9):1497-1500
3. Fried B, Reddy A, Mayer D (2011) Helminths in human carcinogenesis. Cancer Lett 305(2):239-249
4. Lun ZR, Gasser RB, Lai DH, Li AX, Zhu XQ, Yu XB, Fang YY (2005) Clonorchiasis: a key foodborne zoonosis in China. Lancet Infect Dis 5(1):31-41
5. Zhou P, Chen N, Zhang RL, Lin RQ, Zhu XQ (2008) Food-borne parasitic zoonoses in China: perspective for control. Trends Parasitol 24(4):190-196
6. Keiser J, Utzinger J (2009) Food-borne trematodiases. Clin Microbiol Rev 22(3):466-483
7. Luke CJ, Pak SC, Askew YS, Naviglia TL, Askew DJ, Nobar SM, Vetica AC, Long OS, Watkins SC, Stolz DB et al (2007) An intracellular serpin regulates necrosis by inhibiting the induction and sequelae of lysosomal injury. Cell 130(6):1108-1119
8. Irving JA, Steenbakkers PJ, Lesk AM, Op den Camp HJ, Pike RN, Whisstock JC (2002) Serpins in prokaryotes. Mol Biol Evol 19(11):1881-1890
9. Rawlings ND, Tolle DP, Barrett AJ (2004) Evolutionary families of peptidase inhibitors. Biochem J 378(Pt 3):705-716
10. Law RH, Zhang Q, McGowan S, Buckle AM, Silverman GA, Wong W, Rosado CJ, Langendorf CG, Pike RN, Bird PI et al (2006) An overview of the serpin superfamily. Genome Biol 7(5):216
11. Molehin AJ, Gobert GN, McManus DP (2012) Serine protease inhibitors of parasitic helminths. Parasitology 139(6):681-695
12. Mangan MSJ, Kaiserman D, Bird PI (2008) The role of serpins in vertebrate immunity. Tissue Antigens 72(1):1-10
13. Rau JC, Beaulieu LM, Huntington JA, Church FC (2007) Serpins in thrombosis, hemostasis and fibrinolysis. J Thromb Haemost 5(Suppl 1):102-115
14. Gettins PG (2000) Keeping the serpin machine running smoothly. Genome Res 10(12):1833-1835
15. Silverman GA, Bird PI, Carrell RW, Church FC, Coughlin PB, Gettins PG, Irving JA, Lomas DA, Luke CJ, Moyer RW et al (2001) The serpins are an expanding superfamily of structurally similar but functionally diverse proteins. Evolution, mechanism of inhibition, novel functions, and a revised nomenclature. J Biol Chem 276(36):33293-33296
16. Ray CA, Black RA, Kronheim SR, Greenstreet TA, Sleath PR, Salvesen GS, Pickup DJ (1992) Viral inhibition of inflammation: cowpox virus encodes an inhibitor of the interleukin-1 beta converting enzyme. Cell 69(4):597-604
17. Schick C, Pemberton PA, Shi GP, Kamachi Y, Cataltepe S, Bartuski AJ, Gornstein ER, Bromme D, Chapman HA, Silverman GA (1998) Cross-class inhibition of the cysteine proteinases cathepsins K, L, and S by the serpin squamous cell carcinoma antigen 1: a kinetic analysis. Biochemistry 37(15):5258-5266
18. Umasuthan N, Whang I, Kim J-O, Oh M-J, Jung S-J, Choi CY, Yeo S-Y, Lee J-H, Noh JK, Lee J (2011) Rock bream (Oplegnathus fasciatus) serpin, protease nexin-1: transcriptional analysis and characterization of its antiprotease and anticoagulant activities. Dev Comp Immunol 35(7):785-798
19. Zang X, Yazdanbakhsh M, Jiang H, Kanost MR, Maizels RM (1999) A novel serpin expressed by blood-borne microfilariae of the parasitic nematode Brugia malayi inhibits human neutrophil serine proteinases. Blood 94(4):1418-1428
20. Pszenny V, Angel SO, Duschak VG, Paulino M, Ledesma B, Yabo MI, Guarnera E, Ruiz AM, Bontempi EJ (2000) Molecular cloning, sequencing and expression of a serine proteinase inhibitor gene from Toxoplasma gondii. Mol Biochem Parasitol 107(2):241-249
21. Bennuru S, Semnani R, Meng Z, Ribeiro JM, Veenstra TD, Nutman TB (2009) Brugia malayi excreted/secreted proteins at the host/parasite interface: stage- and gender-specific proteomic profiling. PLoS Negl Trop Dis 3(4):e410
22. Yang Y, Hu D, Wang L, Liang C, Hu X, Wang X, Chen J, Xu J, Yu X (2009) Molecular cloning and characterization of a novel serpin gene of Clonorchis sinensis, highly expressed in the stage of metacercaria. Parasitol Res 106(1):221-225
23. Kang J-M, Sohn W-M, Ju J-W, Kim T-S, Na B-K (2010) Identification and characterization of a serine protease inhibitor of Clonorchis sinensis. Acta Trop 116(2):134-140
24. Yoo WG, Kim TI, Li S, Kwon OS, Cho PY, Kim TS, Kim K, Hong SJ (2009) Reference genes for quantitative analysis on Clonorchis sinensis gene expression by real-time PCR. Parasitol Res 104(2):321-328
25. Chen W, Wang X, Deng C, Lv X, Fan Y, Men J, Liang C, Yu X (2011) Molecular cloning and characterization of a novel ras-related protein (rap2) from Clonorchis sinensis. Parasitol Res 108(4):1021-1026
26. Pfaffl MW (2001) A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29(9):e45
27. Deng C, Sun J, Li X, Wang L, Hu X, Wang X, Chen W, Lv X, Liang C, Li W et al (2012) Molecular identification and characterization of leucine aminopeptidase 2, an excretory secretory product of Clonorchis sinensis. Mol Biol Rep 39(10):9817-9826
28. Mohamed SA, Fahmy AS, Mohamed TM, Hamdy SM (2005) Proteases in egg, miracidium and adult of Fasciola gigantica. Characterization of serine and cysteine proteases from adult. Comp Biochem Physiol B: Biochem Mol Biol 142(2):192-200
29. Lv X, Chen W, Wang X, Li X, Sun J, Deng C, Men J, Tian Y, Zhou C, Lei H et al (2012) Molecular characterization and expression of a cysteine protease from Clonorchis sinensis and its application for serodiagnosis of clonorchiasis. Parasitol Res 110(6):2211-2219
30. Hu F, Hu X, Ma C, Zhao J, Xu J, Yu X (2009) Molecular characterization of a novel Clonorchis sinensis secretory phospholipase A(2) and investigation of its potential contribution to hepatic fibrosis. Mol Biochem Parasitol 167(2):127-134
31. Knox DP (2007) Proteinase inhibitors and helminth parasite infection. Parasite Immunol 29(2):57-71
32. MacLennan K, McLean K, Knox DP (2005) Serpin expression in the parasitic stages of Trichostrongylus vitrinus, an ovine intestinal nematode. Parasitology 130(Pt 3):349-357
33. Hwang JH, Lee WG, Na BK, Lee HW, Cho SH, Kim TS (2009) Identification and characterization of a serine protease inhibitor of Paragonimus westermani. Parasitol Res 104(3):495-501
34. Yan Y, Liu S, Song G, Xu Y, Dissous C (2005) Characterization of a novel vaccine candidate and serine proteinase inhibitor from Schistosoma japonicum (Sj serpin). Vet Parasitol 131(1-2):53-60
35. Merckelbach A, Ruppel A (2007) Biochemical properties of an intracellular serpin from Echinococcus multilocularis. Mol Biochem Parasitol 156(1):84-88
36. Yi D, Xu L, Yan R, Li X (2010) Haemonchus contortus: cloning and characterization of serpin. Exp Parasitol 125(4):363-370
37. Lee JS, Kim IS, Sohn WM, Lee J, Yong TS (2006) Vaccination with DNA encoding cysteine proteinase confers protective immune response to rats infected with Clonorchis sinensis. Vaccine 24(13):2358-2366
38. Islam MK, Miyoshi T, Tsuji N (2005) Vaccination with recombinant Ascaris suum 24-kilodalton antigen induces a Th1/Th2-mixed type immune response and confers high levels of protection against challenged Ascaris suum lung-stage infection in BALB/c mice. Int J Parasitol 35(9):1023-1030
39. Chen N, Yuan Z-G, Xu M-J, Zhou D-H, Zhang X-X, Zhang Y-Z, Wang X-W, Yan C, Lin R-Q, Zhu X-Q (2012) Ascaris suum enolase is a potential vaccine candidate against ascariasis. Vaccine 30(23):3478-3482
40. Jiménez-Ruiz E, Álvarez-García G, Aguado-Martínez A, Salman H, Irache JM, Marugán-Hernández V, Ortega-Mora LM (2012) Low efficacy of NcGRA7, NcSAG4, NcBSR4 and NcSRS9 formulated in poly-É-caprolactone against Neospora caninum infection in mice. Vaccine 30(33):4983-4992
41. Pak SC, Tsu C, Luke CJ, Askew YS, Silverman GA (2006) The Caenorhabditis elegans muscle specific serpin, SRP-3, neutralizes chymotrypsin-like serine peptidases. Biochemistry 45(14):4474-4480
42. Pak SC, Kumar V, Tsu C, Luke CJ, Askew YS, Askew DJ, Mills DR, Bromme D, Silverman GA (2004) SRP-2 is a cross-class inhibitor that participates in postembryonic development of the nematode Caenorhabditis elegans: initial characterization of the clade L serpins. J Biol Chem 279(15):15448-15459
43. Wang X, Chen W, Lv X, Tian Y, Men J, Zhang X, Lei H, Zhou C, Lu F, Liang C et al (2012) Identification and characterization of paramyosin from cyst wall of metacercariae implicated protective efficacy against Clonorchis sinensis infection. PLoS One 7(3):e33703