Identification of a mutant α1 Na/K-ATPase that pumps but is defective in signal transduction

Journal of Biological Chemistry

Volumn 288, Issue 19, 2013, Pages 13295-13304

  Lai, Fangfang ^a,b   Madan, Namrata ^a   Ye, Qiqi ^a   Duan, Qiming ^a   Li, Zhichuan ^a   Wang, Shaomeng ^c   Si, Shuyi ^b   Xie, Zijian ^a  

^a MEDICAL COLLEGE OF OHIO   (United States)

^b INSTITUTE OF MEDICINAL BIOTECHNOLOGY   (China)

^c UNIVERSITY OF MICHIGAN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL-BASED ASSAYS; CELL-FREE; LIVE CELL; PHYSIOLOGICAL ROLES; REGULATORY FUNCTIONS; SIGNALING FUNCTIONS;

PUMPS; SIGNAL TRANSDUCTION;

CELL SIGNALING;

ADENOSINE TRIPHOSPHATASE (POTASSIUM SODIUM); ALANINE; ALPHA1 SODIUM POTASSIUM ATPASE; CELL ENZYME; GLUTAMINE; MUTANT PROTEIN; NUCLEOTIDE; PEPTIDE; PROLINE; PROTEIN TYROSINE KINASE; SERINE; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; AMINO TERMINAL SEQUENCE; ARTICLE; CELL GROWTH; CELL STRAIN; CELL STRAIN PY 17; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME REGULATION; ENZYME STRUCTURE; EXPRESSION VECTOR; IN VITRO STUDY; MUTATION; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; SIGNAL TRANSDUCTION; WILD TYPE;

CARDIOTONIC STEROIDS; CELL SIGNALING; MEMBRANE PROTEINS; MUTANT; NA,K-ATPASE; SRC;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMALS; CAVEOLIN 1; CELL LINE; CELL PROLIFERATION; HUMANS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; OUABAIN; PEPTIDE FRAGMENTS; PHOSPHORYLATION; PROTEIN INTERACTION DOMAINS AND MOTIFS; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTEIN STRUCTURE, SECONDARY; RATS; RUBIDIUM RADIOISOTOPES; SIGNAL TRANSDUCTION; SODIUM-POTASSIUM-EXCHANGING ATPASE; SRC-FAMILY KINASES;

EID: 84877689321     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M113.467381     Document Type: Article

References (32)

1. Lingrel, J. B., and Kuntzweiler, T. (1994) Na+,K(+)-ATPase. J. Biol. Chem. 269, 19659-19662
2. +-ATPase. J. Biol. Chem. 275, 27832-27837
3. Liang, M., Cai, T., Tian, J., Qu, W., and Xie, Z. J. (2006) Functional characterization of Src-interacting Na/K-ATPase using RNA interference assay. J. Biol. Chem. 281, 19709-19719
4. +-ATPase forms a functional signaling complex. Mol. Biol. Cell 17, 317-326
5. Liu, J., Yan, Y., Liu, L., Xie, Z., Malhotra, D., Joe, B., and Shapiro, J. I. (2011) Impairment of Na/K-ATPase signaling in renal proximal tubule contributes to Dahl salt-sensitive hypertension. J. Biol. Chem. 286, 22806-22813
6. Liu, J., Kesiry, R., Periyasamy, S. M., Malhotra, D., Xie, Z., and Shapiro, J. I. (2004) Ouabain induces endocytosis of plasmalemmal Na/K-ATPase in LLC-PK1 cells by a clathrin-dependent mechanism. Kidney Int. 66, 227-241
7. Cai, T., Wang, H., Chen, Y., Liu, L., Gunning, W. T., Quintas, L. E., and Xie, Z. J. (2008) Regulation of caveolin-1 membrane trafficking by the Na/K-ATPase. J. Cell Biol. 182, 1153-1169
8. Tian, J., Li, X., Liang, M., Liu, L., Xie, J. X., Ye, Q., Kometiani, P., Tillekeratne, M., Jin, R., and Xie, Z. (2009) Changes in sodium pump expression dictate the effects of ouabain on cell growth. J. Biol. Chem. 284, 14921-14929
9. Li, Z., Cai, T., Tian, J., Xie, J. X., Zhao, X., Liu, L., Shapiro, J. I., and Xie, Z. (2009) NaKtide, a Na/K-ATPase-derived peptide Src inhibitor, antagonizes ouabain-activated signal transduction in cultured cells. J. Biol. Chem. 284, 21066-21076
10. Li, Z., Zhang, Z., Xie, J. X., Li, X., Tian, J., Cai, T., Cui, H., Ding, H., Shapiro, J. I., and Xie, Z. (2011) Na/K-ATPase mimetic pNaKtide peptide inhibits the growth of human cancer cells. J. Biol. Chem. 286, 32394-32403
11. Kawamoto, S. A., Coleska, A., Ran, X., Yi, H., Yang, C. Y., and Wang, S. (2012) Design of triazole-stapled BCL9 α-helical peptides to target the β-catenin/B-cell CLL/lymphoma 9 (BCL9) protein-protein interaction. J. Med. Chem. 55, 1137-1146
12. Liang, M., Tian, J., Liu, L., Pierre, S., Liu, J., Shapiro, J., and Xie, Z. J. (2007) Identification of a pool of non-pumping Na/K-ATPase. J. Biol. Chem. 282, 10585-10593
13. Liu, J., Periyasamy, S. M., Gunning, W., Fedorova, O. V., Bagrov, A. Y., Malhotra, D., Xie, Z., and Shapiro, J. I. (2002) Effects of cardiac glycosides on sodium pump expression and function in LLC-PK1 and MDCK cells. Kidney Int. 62, 2118-2125
14. Shinoda, T., Ogawa, H., Cornelius, F., and Toyoshima, C. (2009) Crystal structure of the sodium-potassium pump at 2.4 A resolution. Nature 459, 446-450
15. +-ATPase. J. Struct. Biol. 174, 296-306
16. Barlow, D. J., and Thornton, J. M. (1988) Helix geometry in proteins. J. Mol. Biol. 201, 601-619
17. Blanco, G. (2005) Na,K-ATPase subunit heterogeneity as a mechanism for tissue-specific ion regulation. Semin. Nephrol. 25, 292-303
18. Chen, Y., Cai, T., Wang, H., Li, Z., Loreaux, E., Lingrel, J. B., and Xie, Z. (2009) Regulation of intracellular cholesterol distribution by Na/K-ATPase. J. Biol. Chem. 284, 14881-14890
19. Li, J., Khodus, G. R., Kruusmägi, M., Kamali-Zare, P., Liu, X. L., Eklöf, A. C., Zelenin, S., Brismar, H., and Aperia, A. (2010) Ouabain protects against adverse developmental programming of the kidney. Nat. Commun. 1, 42
20. +-ATPase) with bound potassium and ouabain. Proc. Natl. Acad. Sci. U.S.A. 106, 13742-13747
21. Boxenbaum, N., Daly, S. E., Javaid, Z. Z., Lane, L. K., and Blostein, R. (1998) Changes in steady-state conformational equilibrium resulting from cytoplasmic mutations of the Na,K-ATPase α-subunit. J. Biol. Chem. 273, 23086-23092
22. +-ATPase. J. Biol. Chem. 278, 38653-38664
23. Parton, R. G., and Simons, K. (2007) The multiple faces of caveolae. Nat. Rev. Mol. Cell Biol. 8, 185-194
24. Salanueva, I. J., Cerezo, A., Guadamillas, M. C., and del Pozo, M. A. (2007) Integrin regulation of caveolin function. J. Cell. Mol. Med. 11, 969-980
25. Parsons, S. J., and Parsons, J. T. (2004) Src family kinases, key regulators of signal transduction. Oncogene 23, 7906-7909
26. Ye, Q., Lai, F., Banerjee, M., Duan, Q., Li, Z., Si, S., and Xie, Z. (2013) Expression of mutant α1 Na/K-ATPase defective in conformational transition attenuates Src-mediated signal transduction. J. Biol. Chem. 288, 5803-5814
27. Bagrov, A. Y., Shapiro, J. I., and Fedorova, O. V. (2009) Endogenous cardiotonic steroids. Physiology, pharmacology, and novel therapeutic targets. Pharmacol. Rev. 61, 9-38
28. +)ATPase associated with essential hypertension. Nature 300, 650-652
29. Kelly, R. A., and Smith, T. W. (1989) The search for the endogenous digitalis. An alternative hypothesis. Am. J. Physiol. 256, C937-C950
30. Dostanic-Larson, I., Van Huysse, J. W., Lorenz, J. N., and Lingrel, J. B. (2005) The highly conserved cardiac glycoside binding site of Na,K-ATPase plays a role in blood pressure regulation. Proc. Natl. Acad. Sci. U.S.A. 102, 15845-15850
31. Loreaux, E. L., Kaul, B., Lorenz, J. N., and Lingrel, J. B. (2008) Ouabainsensitive α1 Na,K-ATPase enhances natriuretic response to saline load. J. Am. Soc. Nephrol. 19, 1947-1954
32. Kennedy, D. J., Vetteth, S., Periyasamy, S. M., Kanj, M., Fedorova, L., Khouri, S., Kahaleh, M. B., Xie, Z., Malhotra, D., Kolodkin, N. I., Lakatta, E. G., Fedorova, O. V., Bagrov, A. Y., and Shapiro, J. I. (2006) Central role for the cardiotonic steroid marinobufagenin in the pathogenesis of experimental uremic cardiomyopathy. Hypertension 47, 488-495