PCR amplification and DNA sequence analysis of parasitic intestinal protozoa in specimens stained with Chlorazol Black E

Acta Parasitologica

Volumn 58, Issue 2, 2013, Pages 132-138

  Morimoto, Norihito ^a   Korenaga, Masataka ^b   Nishida, Yoshie ^a,b   Takeuchi, Hiroaki ^b   Kumon, Yoshitaka ^b   Sugiura, Tetsuro ^a,b  

^a KOCHI MEDICAL SCHOOL   (Japan)

^b KOCHI UNIVERSITY   (Japan)

Author keywords

Chlorazol Black E; genotyping; Giardia intestinalis; intestinal protozoa; Thermococcus kodakaraensis KOD1

Indexed keywords

AZO COMPOUND; DIRECT BLACK 3; PROTOZOAL DNA;

ANIMAL; ARTICLE; CHEMISTRY; DNA SEQUENCE; ENTAMOEBA HISTOLYTICA; EVALUATION STUDY; FECES; GENETICS; GENOTYPE; GIARDIA LAMBLIA; HUMAN; INTESTINE; INTESTINE INFECTION; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; PARASITOLOGY; POLYMERASE CHAIN REACTION; STAINING;

ANIMALS; AZO COMPOUNDS; DNA, PROTOZOAN; ENTAMOEBA HISTOLYTICA; FECES; GENOTYPE; GIARDIA LAMBLIA; HUMANS; INTESTINAL DISEASES, PARASITIC; INTESTINES; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA; STAINING AND LABELING;

EID: 84877619119     PISSN: 12302821     EISSN: None     Source Type: Journal    
DOI: 10.2478/s11686-013-0119-9     Document Type: Article

References (20)

1. Abe N., Kimata I., Tokoro M. 2005. Genotyping of Giardia isolates from humans in Japan using the small subunit ribosomal RNA and glutamate dehydrogenase gene sequences. Japanese Journal of Infectious Diseases, 58, 57-58.
2. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. Basic local alignment search Tool. 1990. Journal of Molecular Biology, 215, 403-410. DOI: 10. 1016/s0022-2836(05)80360-2.
3. Amar C., Pedraza-Díaz S., McLauchlin J. 2001. Extraction and genotyping of Cryptosporidium parvum DNA from fecal smears on glass slides stained conventionally for direct microscope examination. Journal of Clinical Microbiology, 39, 401-403. DOI: 10. 1128/JCM. 39. 1. 401-403. 2001.
4. Amar C. F., Dear P. H., Pedraza-Diaz S., Looker N., Linnane E., McLauchlin J. 2002. Sensitive PCR-restriction fragment length polymorphism assay for detection and genotyping of Giardia duodenalis in human feces. Journal of Clinical Microbiology, 40, 446-452. DOI: 10. 1128/JCM. 40. 2. 446-452. 2002.
5. Bhattacharya D., Haque R., Singh U. 2005. Coding and noncoding genomic regions of Entamoeba histolytica have significantly different rates of sequence polymorphisms: implications for epidemiological studies. Journal of Clinical Microbiology, 43, 4815-4819. DOI: 10. 1128/JCM. 43. 9. 4815-4819. 2005.
6. Gleason N. N., Healy G. R. 1965. Modification and evaluation of Kohn's one-step staining technic for intestinal protozoa in feces or tissue. American Journal of Clinical Pathology, 43, 494-496.
7. Hashimoto H., Nishioka M., Fujiwara S., Takagi M., Imanaka T., Inoue T., Kai Y. 2001. Crystal structure of DNA polymerase from hyperthermophilic archaeon Pyrococcus kodakaraensis KOD1. Journal of Molecular Biology, 306, 469-477. DOI: 10. 1006/jmbi. 2000. 4403.
8. Hayashida M., Iwao-Koizumi K., Murata S., Kinoshita K. 2009. Singletube genotyping from a human hair root by direct PCR. Analytical Sciences, 25, 1487. DOI: 10. 2116/analsci. 25. 1487.
9. Kim Y. A., Kim H. Y., Cho S. H., Cheun H. I., Yu J. R., Lee S. E. 2010. PCR detection and molecular characterization of Pentatrichomonas hominis from feces of dogs with diarrhea in the Republic of Korea. Korean Journal of Parasitology, 48, 9-13. DOI: 10. 3347/kjp. 2010. 48. 1. 9.
10. Kohn J. 1960. A one stage permanent staining method for faecal protozoa. Dapim Refuiim 19: IV-V.
11. Lalle M. 2010. Giardiasis in the post genomic era: treatment, drug resistance and novel therapeutic perspectives. Infectious Disorders Drug Targets, 10, 283-294. DOI: 10. 2174/187152610791591610.
12. Matsubayashi M., Kimata I., Abe N. 2005. Identification of genotypes of Giardia intestinalis isolates from a human and calf in Japan. Journal of Veterinary Medical Science, 67, 337-340. DOI: 10. 1292/jvms. 67. 337.
13. Molina N., Polverino D., Minvielle M., Basualdo J. 2007. PCR amplification of triosephosphate isomerase gene of Giardia lam blia in formalin-fixed feces. Revista Latinoamericana de Microbiología, 49, 6-11.
14. Ramos F., Zurabian R., Morán P., Ramiro M., Gómez A., Clark C. G., Melendro E. I., García G., Ximénez C. 1999. The effect of formalin fixation on the polymerase chain reaction characterization of Entamoeba histolytica. Transactions of the Royal Society of Tropical Medicine and Hygiene, 93, 335-336. DOI: 10. 1016/S0035-9203(99)90045-7.
15. Tachibana H., Kobayashi S., Takekoshi M., Ihara S. 1991. Distinguishing pathogenic isolates of Entamoeba histolytica by polymerase chain reaction. Journal of Infectious Diseases, 164, 825-826. DOI: 10. 1093/infdis/164. 4. 825.
16. Takagi M., Nishioka M., Kakihara H., Kitabayashi M., Inoue H., Kawakami B., Oka M., Imanaka T. 1997. Characterization of DNA polymerase from Pyrococcus sp. strain KOD1 and its application to PCR. Applied and Environmental Microbiology, 63, 4504-4510.
17. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 25, 4876-4882. DOI: 10. 1093/nar/25. 24. 4876.
18. Traub R. J., Monis P. T., Robertson I., Irwin P., Mencke N., Thompson R. C. 2004. Epidemiological and molecular evidence supports the zoonotic transmission of Giardia among humans and dogs living in the same community. Parasitology, 128, 253-262. DOI: 10. 1017/S0031182003004505.
19. Troll H., Marti H., Weiss N. 1997. Simple Differential detection of Entamoeba histolytica and Entamoeba dispar in fresh stool specimens by sodium acetate-acetic acid-formalin concentration and PCR. Journal of Clinical Microbiology, 35, 1701-1705.
20. van der Giessen J. W., de Vries A., Roos M., Wielinga P., Kortbeek L. M., Mank T. G. 2006. Genotyping of Giardia in Dutch patients and animals: a phylogenetic analysis of human and animal isolates. International Journal for Parasitology, 36, 849-858. DOI: 10. 1016/j. ijpara. 2006. 03. 001.