SpoIIIE mechanism of directional translocation involves target search coupled to sequence-dependent motor stimulation

EMBO Reports

Volumn 14, Issue 5, 2013, Pages 473-479

  Cattoni, Diego I ^a   Chara, Osvaldo ^b,c   Godefroy, Cédric ^a   Margeat, Emmanuel ^a   Trigueros, Sonia ^d   Milhiet, Pierre Emmanuel ^a   Nöllmann, Marcelo ^a  

^a CENTRE DE BIOCHIMIE STRUCTURALE   (France)

^b UNIVERSIDAD NACIONAL DE LA PLATA   (Argentina)

^c DRESDEN UNIVERSITY OF TECHNOLOGY   (Germany)

^d UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

DNA segregation; molecular motors; sequence specific regulation; single molecule; translocation directionality

Indexed keywords

ADENOSINE TRIPHOSPHATE; BACTERIAL DNA; MOLECULAR MOTOR; PROTEIN; PROTEIN FTSK GAMMA; PROTEIN SPOIIIE; PROTEIN SPOIIIE GAMMA; UNCLASSIFIED DRUG;

ANISOTROPY; ARTICLE; BINDING AFFINITY; CHROMOSOME SEGREGATION; DNA SEQUENCE; MOLECULAR BIOLOGY; NONHUMAN; OLIGOMERIZATION; PRIORITY JOURNAL; PROTEIN DNA BINDING;

ANISOTROPY; BACTERIAL PROTEINS; BASE SEQUENCE; BINDING SITES; DNA, BACTERIAL; KINETICS; MICROSCOPY, ATOMIC FORCE; MODELS, MOLECULAR; PROTEIN BINDING; PROTEIN TRANSPORT; SPECTROMETRY, FLUORESCENCE;

EID: 84877585573     PISSN: 1469221X     EISSN: 14693178     Source Type: Journal    
DOI: 10.1038/embor.2013.39     Document Type: Article

References (18)

1. Pease PJ, Levy O, Cost GJ, Gore J, Ptacin JL, Sherratt D, Bustamante C, Cozzarelli NR (2005) Sequence-directed DNA translocation by purified FtsK. Science 307: 586-590 (Pubitemid 40165682)
2. Ptacin JL, Nollmann M, Becker EC, Cozzarelli NR, Pogliano K, Bustamante C (2008) Sequence-directed DNA export guides chromosome translocation during sporulation in Bacillus subtilis. Nat Struct Mol Biol 15: 485-493 (Pubitemid 351653585)
3. Vogelmann J, Ammelburg M, Finger C, Guezguez J, Linke D, Flotenmeyer M, Stierhof YD, Wohlleben W, Muth G (2011) Conjugal plasmid transfer in Streptomyces resembles bacterial chromosome segregation by FtsK/SpoIIIE. EMBO J 30: 2246-2254
4. Levy O, Ptacin JL, Pease PJ, Gore J, Eisen MB, Bustamante C, Cozzarelli NR (2005) Identification of oligonucleotide sequences that direct the movement of the Escherichia coli FtsK translocase. Proc Natl Acad Sci USA 102: 17618-17623 (Pubitemid 41803546)
5. Bigot S et al (2005) KOPS: DNA motifs that control E. coli chromosome segregation by orienting the FtsK translocase. EMBO J 24: 3770-3780 (Pubitemid 41581693)
6. Lowe J, Ellonen A, Allen MD, Atkinson C, Sherratt DJ, Grainge I (2008) Molecular mechanism of sequence-directed DNA loading and translocation by FtsK. Mol Cell 31: 498-509
7. Bigot S, Saleh OA, Cornet F, Allemand JF, Barre FX (2006) Oriented loading of FtsK on KOPS. Nat Struct Mol Biol 13: 1026-1028 (Pubitemid 44684856)
8. Graham JE, Sherratt DJ, Szczelkun MD (2010) Sequence-specific assembly of FtsK hexamers establishes directional translocation on DNA. Proc Natl Acad Sci USA 107: 20263-20268
9. Lakowicz JR (2006) Principles of Fluorescence Spectroscopy. New York: Springer
10. Ortega A, Amoros D, Garcia de la Torre J (2011) Prediction of hydrodynamic and other solution properties of rigid proteins from atomic- and residue-level models. Biophys J 101: 892-898
11. Massey TH, Mercogliano CP, Yates J, Sherratt DJ, Lowe J (2006) Doublestranded DNA translocation: structure and mechanism of hexameric FtsK. Mol Cell 23: 457-469 (Pubitemid 44205486)
12. Halford SE, Marko JF (2004) How do site-specific DNA-binding proteins find their targets? Nucleic Acids Res 32: 3040-3052 (Pubitemid 39022995)
13. Burton BM, Marquis KA, Sullivan NL, Rapoport TA, Rudner DZ (2007) The ATPase SpoIIIE transports DNA across fused septal membranes during sporulation in Bacillus subtilis. Cell 131: 1301-1312 (Pubitemid 350297414)
14. Skordalakes E, Berger JM (2006) Structural insights into RNA-dependent ring closure and ATPase activation by the Rho termination factor. Cell 127: 553-564 (Pubitemid 44647425)
15. Lee JY, Finkelstein IJ, Crozat E, Sherratt DJ, Greene EC (2012) Singlemolecule imaging of DNA curtains reveals mechanisms of KOPS sequence targeting by the DNA translocase FtsK. Proc Natl Acad Sci USA 109: 6531-6536
16. Wagner T, Bellinzoni M, Wehenkel A, O'Hare HM, Alzari PM (2011) Functional plasticity and allosteric regulation of a-ketoglutarate decarboxylase in central mycobacterial metabolism. Chem Biol 18: 1011-1020
17. Mott ML, Berger JM (2007) DNA replication initiation: mechanisms and regulation in bacteria. Nat Rev Microbiol 5: 343-354 (Pubitemid 46605488)
18. Royer CA, Smith WR, Beechem JM (1990) Analysis of binding in macromolecular complexes: a generalized numerical approach. Anal Biochem 191: 287-294