Sequence-specific assembly of FtsK hexamers establishes directional translocation on DNA

Proceedings of the National Academy of Sciences of the United States of America

Volumn 107, Issue 47, 2010, Pages 20263-20268

  Graham, James E ^a,c   Sherratt, David J ^a   Szczelkun, Mark D ^b  

^a UNIVERSITY OF OXFORD   (United Kingdom)

^b UNIVERSITY OF BRISTOL   (United Kingdom)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

ASCE ATPase; DNA translocation; FtsK translocase; Stopped flow; Triplex displacement

Indexed keywords

ADENOSINE TRIPHOSPHATASE; ADENOSINE TRIPHOSPHATE; BACTERIAL DNA; CARRIER PROTEIN; FTSK ENZYME; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; BACTERIAL CHROMOSOME; BACTERIAL TRANSLOCATION; CHROMATIN ASSEMBLY AND DISASSEMBLY; CONFERENCE PAPER; ENZYME ASSAY; ENZYME KINETICS; HYDROLYSIS; MOLECULAR INTERACTION; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA;

BACTERIA (MICROORGANISMS);

EID: 78650535019     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1007518107     Document Type: Article

References (24)

1. Bigot S, et al. (2007) FtsK, a literate chromosome segregation machine. Mol Microbiol 64:1434-1441.
2. Bigot S, et al. (2006) Oriented loading of FtsK on KOPS. Nat Struct Mol Biol 13:1026-1028.
3. Bigot S, et al. (2005) KOPS: DNA motifs that control E. coli chromosome segregation by orienting the FtsK translocase. EMBO J 24:3770-3780.
4. Saleh OA, Pérals C, Barre F-X, Allemand J-F (2004) Fast, DNA-sequence independent translocation by FtsK in a single-molecule experiment. EMBO J 23:2430-2439.
5. Ptacin JL, Nöllmann M, Bustamante C, Cozzarelli NR (2006) Identification of the FtsK sequence-recognition domain. Nat Struct Mol Biol 13:1023-1025.
6. Pease PJ, et al. (2005) Sequence-directed DNA translocation by purified FtsK. Science 307:586-590.
7. Levy O, et al. (2005) Identification of oligonucleotide sequences that direct the movement of the Escherichia coli FtsK translocase. Proc Natl Acad Sci USA 102:17618-17623.
8. Löwe J, et al. (2008) Molecular mechanism of sequence-directed DNA loading and translocation by FtsK. Mol Cell 31:498-509.
9. Sivanathan V, et al. (2006) The FtsK gamma domain directs oriented DNA translocation by interacting with KOPS. Nat Struct Mol Biol 13:965-972.
10. Massey TH, et al. (2006) Double-stranded DNA translocation: structure and mechanism of hexameric FtsK. Mol Cell 23:457-469.
11. Singleton MR, Dillingham MS, Wigley DB (2007) Structure and mechanism of helicases and nucleic acid translocases. Annu Rev Biochem 76:23-50.
12. Enemark E, Joshua-Tor L (2008) On helicases and other motor proteins. Curr Opin Struct Biol 18:243-257.
13. Firman K, Szczelkun MD (2000) Measuring motion on DNA by the type I restriction endonuclease EcoR124I using triplex displacement. EMBO J 19:2094-2102.
14. McClelland SE, Dryden DTF, Szczelkun MD (2005) Continuous assays for DNA translocation using fluorescent triplex dissociation: application to type I restriction endonucleases. J Mol Biol 348:895-915.
15. Seidel R, Bloom J, Dekker C, Szczelkun M (2008) Motor step size and ATP coupling efficiency of the dsDNA translocase EcoR124I. EMBO J 27:1388-1398.
16. Webb MR (2007) Development of fluorescent biosensors for probing the function of motor proteins. Mol Biosyst 3:249-256.
17. Smith RM, Josephsen J, Szczelkun MD (2009) The single polypeptide restrictionmodification enzyme LlaGI is a self-contained molecular motor that translocates DNA loops. Nucleic Acids Res 37:7219-7230.
18. Neuhard J, Nygaard P (1987) Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology, eds F Neidhardt et al. (ASM Press, Washington, DC).
19. Roman LJ, Kowalczykowski SC (1989) Characterization of the adenosinetriphosphatase activity of the Escherichia coli RecBCD enzyme: relationship of ATP hydrolysis to the unwinding of duplex DNA. Biochemistry 28:2873-2881.
20. Thomsen ND, Berger JM (2009) Running in reverse: the structural basis for translocation polarity in hexameric helicases. Cell 139:523-534.
21. Saikrishnan K, et al. (2009) Mechanistic basis of 5'-3' translocation in SF1B helicases. Cell 137:849-859.
22. Crozat E, et al. (2010) Separating speed and ability to displace roadblocks during DNA translocation by FtsK. EMBO J 29:1423-1433.
23. Enemark EJ, Joshua-Tor L (2006) Mechanism of DNA translocation in a replicative hexameric helicase. Nature 442:270-275.
24. Seidel R, et al. (2005) Dynamics of initiation, termination, and reinitiation of DNA translocation by the motor protein EcoR124I. EMBO J 24:4188-4197.