Purifying common light-chain bispecific antibodies

BioProcess International

Volumn 11, Issue 5, 2013, Pages 36-45

  Müller Späth, Thomas ^a   Ulmer, Nicole ^a   Aumann, Lars ^a   Ströhlein, Guido ^a   Bavand, Michael ^a   Hendriks, Linda J A ^b   De Kruif, John ^b   Throsby, Mark ^b   Bakker, Alexander B H ^b  

^a CHROMACON AG   (Switzerland)

^b Merus Biopharmaceuticals   (Netherlands)

Author keywords

Affinity capture; Analytical chromatography; Bind elute; Flow through; Ion exchange polishing; Process chromatography

Indexed keywords

BISPECIFIC ANTIBODY; IMMUNOGLOBULIN G;

ADSORPTION; ANALYTIC METHOD; ANTIBODY AFFINITY; ANTIBODY ENGINEERING; ANTIBODY SPECIFICITY; ARTICLE; CONTROLLED STUDY; COUNTER CURRENT CHROMATOGRAPHY; ELUTION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; INTERMETHOD COMPARISON; LIGHT CHAIN; MULTICOLUMN COUNTERCURRENT SOLVENT GRADIENT PURIFICATION CHROMATOGRAPHY; PROTEIN PURIFICATION;

EID: 84877323859     PISSN: 15426319     EISSN: None     Source Type: Trade Journal    
DOI: None     Document Type: Article

References (7)

1. Gunasekaran K, et al. Enhancing Antibody Fc Heterodimer Formation Through Electrostatic Steering Effects: Applications to Bispecific Molecules and Monovalent IgG. J. Biol. Chem. 285, 2010: 19637-19646.
2. Cain C. Crossing Over to Bispecificity. SciBX 4(28) 2011: 783.
3. Rosati S, et al. Qualitative and Semiquantitative Analysis of Composite Mixtures of Antibodies By Native Mass Spectrometry. Analyt. Chem. 84(16) 2012: 7227-7232.
4. Aumann L, Morbidelli M. Method and Device for Chromatographic Purification. Eidgenössische Technische Hochschule: Zürich, Switzerland, 9 November 2006: WO 2006/116886WO/2006/116886.
5. Aumann L, Morbidelli M. A Continuous Multicolumn Countercurrent Solvent Gradient Purification (MCSGP) Process. Biotechnol. Bioeng. 98(5) 2007: 1043-1055. (Pubitemid 350205581)
6. Müller-Späth T, et al. Chromatographic Separation of Three Monoclonal Antibody Variants Using Multicolumn Countercurrent Solvent Gradient Purification (MCSGP). Biotechnol. Bioeng. 100(6) 2008: 1166-1177.
7. Müller-Späth T, et al. Increasing the Activity of Monoclonal Antibody Therapeutics By Continuous Chromatography (MCSGP). Biotechnol. Bioeng. 107(4) 2010: 652-662.