Preparation and characterization of SNARE-containing nanodiscs and direct study of cargo release through fusion pores

Nature Protocols

Volumn 8, Issue 5, 2013, Pages 935-948

  Shi, Lei ^a   Howan, Kevin ^b   Shen, Qing Tao ^c   Wang, Yong Jian ^b   Rothman, James E ^a   Pincet, Frédéric ^a,b  

^a YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b ECOLE NORMALE SUPÉRIEURE   (France)

^c YALE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DITHIONITE; HYBRID PROTEIN; LIPID; LIPOSOME; NANODISC; SCAFFOLD PROTEIN; SNARE PROTEIN; SYNAPTOBREVIN 2; SYNAPTOSOMAL ASSOCIATED PROTEIN 25; SYNTAXIN 1;

ARTICLE; DATA ANALYSIS; FLUORESCENCE ANALYSIS; IN VITRO STUDY; LIPID BILAYER; NANOANALYSIS; NANOENCAPSULATION; NANOPORE; PARTICLE SIZE; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN ISOLATION; PROTEIN LOCALIZATION; PROTEIN SECRETION; PROTEIN STRUCTURE; PROTEIN TRANSPORT; QUANTITATIVE ANALYSIS; SENSITIVITY ANALYSIS; SEQUENCE ANALYSIS;

BIOLOGICAL TRANSPORT; LIPOSOMES; MEMBRANE FUSION; MICROSCOPY, FLUORESCENCE; MODELS, BIOLOGICAL; NANOSTRUCTURES; SYNAPTOSOMAL-ASSOCIATED PROTEIN 25; VESICLE-ASSOCIATED MEMBRANE PROTEIN 2;

EID: 84877048781     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2013.048     Document Type: Article

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