A preclinical xenograft model of prostate cancer using human tumors

Nature Protocols

Volumn 8, Issue 5, 2013, Pages 836-848

  Lawrence, Mitchell G ^a   Taylor, Renea A ^a   Toivanen, Roxanne ^a   Pedersen, John ^b   Norden, Sam ^b   Pook, David W ^a   Frydenberg, Mark ^a   Papargiris, Melissa M ^a   Niranjan, Birunthi ^a   Richards, Michelle G ^a   Wang, Hong ^a   Collins, Anne T ^c   Maitland, Norman J ^c   Risbridger, Gail P ^a  

^a MONASH UNIVERSITY   (Australia)

^b Tissupath Pty Ltd   (Australia)

^c UNIVERSITY OF YORK   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CD133 ANTIGEN; VERY LATE ACTIVATION ANTIGEN 2;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CANCER CELL CULTURE; CANCER LOCALIZATION; CANCER TISSUE; CELL INTERACTION; CELL PROLIFERATION; CELL SELECTION; CONTROLLED STUDY; FEMALE; FIBROBLAST; HUMAN; HUMAN CELL; HUMAN TISSUE; KIDNEY CAPSULE; MALE; MESENCHYMAL STROMA CELL; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROSTATE CANCER; PROSTATE EPITHELIUM; RAT; SEMINAL VESICLE; SIGNAL TRANSDUCTION; TUMOR XENOGRAFT;

ANIMALS; CELL CULTURE TECHNIQUES; CELL SEPARATION; COCULTURE TECHNIQUES; DRUG EVALUATION, PRECLINICAL; HUMANS; KIDNEY; MALE; MICE; MICE, SCID; PROSTATIC NEOPLASMS; SEMINAL VESICLES; TRANSPLANTATION, HETEROLOGOUS; XENOGRAFT MODEL ANTITUMOR ASSAYS;

MUS;

EID: 84877047730     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2013.043     Document Type: Article

References (36)

1. Toivanen, R., Taylor, R.A., Pook, D.W., Ellem, S.J. & Risbridger, G.P. Breaking through a roadblock in prostate cancer research: An update on human model systems. J. Steroid Biochem. Mol. Biol. 131, 122-131 (2012
2. Maitland, N.J., Frame, F.M., Polson, E.S., Lewis, J.L. & Collins, A.T. Prostate cancer stem cells: Do they have a basal or luminal phenotype? Horm. Cancer 2, 47-61 (2011
3. Hayward, S.W., Rosen, M.A. & Cunha, G.R. Stromal-epithelial interactions in the normal and neoplastic prostate. Br. J. Urol. 79 (suppl. 2), 18-26 (1997
4. Cunha, G.R. Epithelio-mesenchymal interactions in primordial gland structures which become responsive to androgenic stimulation. Anat. Rec. 172, 179-195 (1972
5. Goldstein, A.S. et al. Purification and direct transformation of epithelial progenitor cells from primary human prostate. Nat. Protoc. 6, 656-667 (2011
6. Lukacs, R.U., Goldstein, A.S., Lawson, D.A., Cheng, D. & Witte, O.N. Isolation, cultivation and characterization of adult murine prostate stem cells. Nat. Protoc. 5, 702-713 (2010
7. Taylor, R.A. et al. Formation of human prostate tissue from embryonic stem cells. Nat. Methods 3, 179-181 (2006
8. Taylor, R.A. et al. Lineage enforcement by inductive mesenchyme on adult epithelial stem cells across developmental germ layers. Stem Cells 27, 3032-3042 (2009
9. Toivanen, R. et al. Brief report: A bioassay to identify primary human prostate cancer repopulating cells. Stem Cells 29, 1310-1314 (2011
10. Collins, A.T., Habib, F.K., Maitland, N.J. & Neal, D.E. Identification and isolation of human prostate epithelial stem cells based on α(2)β(1)-integrin expression. J. Cell Sci. 114, 3865-3872 (2001
11. Collins, A.T., Berry, P.A., Hyde, C., Stower, M.J. & Maitland, N.J. Prospective identification of tumorigenic prostate cancer stem cells. Cancer Res. 65, 10946-10951 (2005
12. Richardson, G.D. et al. CD133, a novel marker for human prostatic epithelial stem cells. J. Cell Sci. 117, 3539-3545 (2004
13. Hayashi, N., Cunha, G.R. & Wong, Y.C. Influence of male genital tract mesenchymes on differentiation of Dunning prostatic adenocarcinoma. Cancer Res. 50, 4747-4754 (1990
14. Chung, L.W., Zhau, H.E. & Ro, J.Y. Morphologic and biochemical alterations in rat prostatic tumors induced by fetal urogenital sinus mesenchyme. Prostate 17, 165-174 (1990
15. Tuxhorn, J.A. et al. Reactive stroma in human prostate cancer: Induction of myofibroblast phenotype and extracellular matrix remodeling. Clin. Cancer Res. 8, 2912-2923 (2002
16. Olumi, A.F. et al. Carcinoma-associated fibroblasts direct tumor progression of initiated human prostatic epithelium. Cancer Res. 59, 5002-5011 (1999
17. Ayala, G. et al. Reactive stroma as a predictor of biochemical-free recurrence in prostate cancer. Clin. Cancer Res. 9, 4792-4801 (2003
18. Jia, Z. et al. Diagnosis of prostate cancer using differentially expressed genes in stroma. Cancer Res. 71, 2476-2487 (2011
19. Taylor, R.A. et al. Human epithelial basal cells are cells of origin of prostate cancer, independent of CD133 status. Stem Cells 30, 1087-1096 (2012
20. Zhao, H., Nolley, R., Chen, Z. & Peehl, D.M. Tissue slice grafts: An in vivo model of human prostate androgen signaling. Am. J. Pathol. 177, 229-239 (2010
21. van Weerden, W.M. & Romijn, J.C. Use of nude mouse xenograft models in prostate cancer research. Prostate 43, 263-271 (2000
22. Wang, Y. et al. Development and characterization of efficient xenograft models for benign and malignant human prostate tissue. Prostate 64, 149-159 (2005
23. Priolo, C. et al. Establishment and genomic characterization of mouse xenografts of human primary prostate tumors. Am. J. Pathol. 176, 1901-1913 (2010
24. Wang, Y. et al. An orthotopic metastatic prostate cancer model in SCID mice via grafting of a transplantable human prostate tumor line. Lab. Invest. 85, 1392-1404 (2005
25. Lin, D. et al. ASAP1, a gene at 8q24, is associated with prostate cancer metastasis. Cancer Res. 68, 4352-4359 (2008
26. Goldstein, A.S., Huang, J., Guo, C., Garraway, I.P. & Witte, O.N. Identification of a cell of origin for human prostate cancer. Science 329, 568-571 (2010
27. Ricke, W.A. et al. Prostatic hormonal carcinogenesis is mediated by in situ estrogen production and estrogen receptor alpha signaling. FASEB J. 22, 1512-1520 (2008
28. Wang, Y. et al. A human prostatic epithelial model of hormonal carcinogenesis. Cancer Res. 61, 6064-6072 (2001
29. Hu, W.Y. et al. Estrogen-initiated transformation of prostate epithelium derived from normal human prostate stem-progenitor cells. Endocrinology 152, 2150-2163 (2011
30. Lin, D. et al. Development of metastatic and non-metastatic tumor lines from a patient′s prostate cancer specimen-identification of a small subpopulation with metastatic potential in the primary tumor. Prostate 70, 1636-1644 (2010
31. Logothetis, C.J., Navone, N.M. & Lin, S.H. Understanding the biology of bone metastases: Key to the effective treatment of prostate cancer. Clin. Cancer Res. 14, 1599-1602 (2008
32. Raheem, O. et al. A novel patient-derived intrafemoral xenograft model of bone metastatic prostate cancer that recapitulates mixed osteolytic and osteoblastic lesions. J. Transl. Med. 9, 185 (2011
33. Kerger, M. et al. Microscopic assessment of fresh prostate tumour specimens yields significantly increased rates of correctly annotated samples for downstream analysis. Pathology 44, 204-208 (2012
34. Szot, G.L., Koudria, P. & Bluestone, J.A. Transplantation of pancreatic islets into the kidney capsule of diabetic mice. J. Vis. Exp. 9, e404 (2007
35. Ritner, C. & Bernstein, H.S. Fate mapping of human embryonic stem cells by teratoma formation. J. Vis. Exp. 42, e2036 (2010
36. Joesting, M.S. et al. Identification of SFRP1 as a candidate mediator of stromal-to-epithelial signaling in prostate cancer. Cancer Res. 65, 10423-10430 (2005