Characterization of a short isoform of the kidney protein podocin in human kidney

BMC Nephrology

Volumn 14, Issue 1, 2013, Pages

  Völker, Linus A ^a   Schurek, Eva Maria ^a   Rinschen, Markus M ^a   Tax, Judit ^a   Schutte, Barbara A ^a   Lamkemeyer, Tobias ^a   Ungrue, Denise ^a   Schermer, Bernhard ^a   Benzing, Thomas ^a   Höhne, Martin ^a  

^a UNIVERSITY OF COLOGNE   (Germany)

Author keywords

Isoform; Kidney glomerulus; Podocin

Indexed keywords

COMPLEMENTARY DNA; DETERGENT; GLYCOPEPTIDASE; MEMBRANE PROTEIN; PODOCIN; SUCROSE;

ALTERNATIVE RNA SPLICING; ARTICLE; CELL LYSATE; CONTROLLED STUDY; DATA BASE; DNA LIBRARY; DNA SEQUENCE; ENZYME ASSAY; FEMALE; GENE EXPRESSION; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE TEST; KIDNEY; MASS SPECTROMETRY; PROTEIN ANALYSIS; PROTEIN GLYCOSYLATION; PROTEIN URINE LEVEL; SUCROSE DENSITY GRADIENT CENTRIFUGATION;

AMINO ACID SEQUENCE; HEK293 CELLS; HELA CELLS; HUMANS; INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS; KIDNEY; MEMBRANE PROTEINS; MOLECULAR SEQUENCE DATA; PROTEIN ISOFORMS;

EID: 84877037040     PISSN: None     EISSN: 14712369     Source Type: Journal    
DOI: 10.1186/1471-2369-14-102     Document Type: Article

References (17)

1. NPHS2, encoding the glomerular protein podocin, is mutated in autosomal recessive steroid-resistant nephrotic syndrome. Boute N, Gribouval O, Roselli S, Benessy F, Lee H, Fuchshuber A, Dahan K, Gubler M-C, Niaudet P, Antignac C, Nat Genet 2000 24 349 354 10.1038/74166 10742096 (Pubitemid 30187431)
2. Podocin, a raft-associated component of the glomerular slit diaphragm, interacts with CD2AP and nephrin. Schwarz K, Simons M, Reiser J, Saleem MA, Faul C, Kriz W, Shaw AS, Holzman LB, Mundel P, J Clin Invest 2001 108 1621 1629 11733557 (Pubitemid 33144872)
3. Early Glomerular Filtration Defect and Severe Renal Disease in Podocin-Deficient Mice. Roselli S, Heidet L, Sich M, Henger A, Kretzler M, Gubler M-C, Antignac C, Mol Cell Biol 2004 24 550 560 10.1128/MCB.24.2.550-560. 2004 14701729 (Pubitemid 38057908)
4. Podocin and MEC-2 bind cholesterol to regulate the activity of associated ion channels. Huber TB, Schermer B, Müller RU, Höhne M, Bartram M, Calixto A, Hagmann H, Reinhardt C, Koos F, Kunzelmann K, Shirokova E, Krautwurst D, Harteneck C, Simons M, Pavenstädt H, Kerjaschki D, Thiele C, Walz G, Chalfie M, Benzing T, Proc Natl Acad Sci USA 2006 103 17079 17086 10.1073/pnas.0607465103 17079490 (Pubitemid 44788925)
5. Lipid-Protein Interactions along the Slit Diaphragm of Podocytes. Schermer B, Benzing T, J Am Soc Nephrol 2009 20 473 478 10.1681/ASN.2008070694 19244577
6. In situ evaluation of podocin in normal and glomerular diseases. Horinouchi I, Nakazato H, Kawano T, Iyama K, Furuse A, Arizono K, Machida J, Sakamoto T, Endo F, Hattori S, Kidney Int 2003 64 2092 2099 10.1046/j.1523-1755. 2003.00303.x 14633131 (Pubitemid 37449543)
7. New perspectives on the renal slit diaphragm protein podocin. Relle M, Cash H, Brochhausen C, Strand D, Menke J, Galle PR, Schwarting A, Mod Pathol 2011 24 1101 1110 10.1038/modpathol.2011.58 21499232
8. Two-stage PCR protocol allowing introduction of multiple mutations, deletions and insertions using QuikChange Site-Directed Mutagenesis. Wang W, Malcolm BA, Biotechniques 1999 26 680 682 10343905
9. Isolation and Enrichment of Glomeruli Using Sieving Techniques. Yamamoto T, Renal and Urinary Proteomics: Methods and Protocols Weinheim: Wiley-VCH Verlag, Thongboonkerd V, 1 2009 1 7
10. Protocol for micro-purification, enrichment, pre-fractionation and storage of peptides for proteomics using StageTips. Rappsilber J, Mann M, Ishihama Y, Nat Protoc 2007 2 1896 1906 10.1038/nprot.2007.261 17703201 (Pubitemid 47308128)
11. Quantitative phosphoproteomic analysis reveals vasopressin V2-receptor-dependent signaling pathways in renal collecting duct cells. Rinschen MM, Yu M-J, Wang G, Boja ES, Hoffert JD, Pisitkun T, Knepper MA, Proc Natl Acad Sci USA 2010 107 3882 3887 10.1073/pnas.0910646107 20139300
12. Automated quantification tool for high-throughput proteomics using stable isotope labeling and LC-MSn. Wang G, Wu WW, Pisitkun T, Hoffert JD, Knepper MA, Shen R-F, Anal Chem 2006 78 5752 5761 10.1021/ac060611v 16906720 (Pubitemid 44265036)
13. MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification. Cox J, Mann M, Nat Biotechnol 2008 26 1367 1372 10.1038/nbt.1511 19029910
14. Molecular basis of the functional podocin-nephrin complex: mutations in the NPHS2 gene disrupt nephrin targeting to lipid raft microdomains. Huber TB, Simons M, Hartleben B, Sernetz L, Schmidts M, Gundlach E, Saleem MA, Walz G, Benzing T, Hum Mol Genet 2003 12 3397 3405 10.1093/hmg/ddg360 14570703 (Pubitemid 37541084)
15. Plasma Membrane Targeting of Podocin Through the Classical Exocytic Pathway: Effect of NPHS2 Mutations. Roselli S, Moutkine I, Gribouval O, Benmerah A, Antignac C, Traffic 2004 5 37 44 10.1046/j.1600-0854.2003.00148.x 14675423 (Pubitemid 38159402)
16. Signaling at the Slit Diaphragm. Benzing T, J Am Soc Nephrol 2004 15 1382 1391 10.1097/01.ASN.0000130167.30769.55 15153549 (Pubitemid 38679663)
17. A single conserved proline residue determines the membrane topology of stomatin. Kadurin I, Huber S, Gründer S, Biochem J 2009 418 587 594 10.1042/BJ20081662 19032151