In vitro imaging of primary neural cell culture from Drosophila

Nature Protocols

Volumn 8, Issue 5, 2013, Pages 958-965

  Egger, Boris ^a   Van Giesen, Lena ^a   Moraru, Manuela ^a,b   Sprecher, Simon G ^a  

^a UNIVERSITY OF FRIBOURG   (Switzerland)

^b EPFL   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

ACETYLCHOLINE; CALCIUM;

ANIMAL CELL; ANIMAL TISSUE; ARTHROPOD LARVA; ARTICLE; BIOSENSOR; BRAIN DEVELOPMENT; CELL ACTIVITY; CELL FUNCTION; CELL LABELING; CELL PROLIFERATION; CELL SPECIFICITY; CELL STRUCTURE; CELL TYPE; CELLULAR DISTRIBUTION; CONFOCAL MICROSCOPE; CONTROLLED STUDY; DROSOPHILA MELANOGASTER; EMBRYO; GENOTYPING TECHNIQUE; GLIA CELL; IMMUNOFLUORESCENCE TEST; IN VITRO STUDY; IN VIVO STUDY; MOLECULAR DYNAMICS; MOLECULAR IMAGING; NERVE CELL CULTURE; NERVE CELL DIFFERENTIATION; NEURAL STEM CELL; NONHUMAN; PRIORITY JOURNAL;

ANIMALS; CELL CULTURE TECHNIQUES; DROSOPHILA MELANOGASTER; EMBRYO, NONMAMMALIAN; LARVA; MICROSCOPY, FLUORESCENCE; NEURONS;

ANIMALIA; DROSOPHILA MELANOGASTER;

EID: 84877020246     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2013.052     Document Type: Article

References (38)

1. Campos-Ortega, J. & Hartenstein, V. The Embryonic Development of Drosophila melanogaster 2nd edn. (Springer, 1997
2. Schneider, I. Cell lines derived from late embryonic stages of Drosophila melanogaster. J. Embryol. Exp. Morphol. 27, 353-365 (1972
3. Echalier, G. & Ohanessian, A. Isolation, in tissue culture, of Drosophila melangaster cell lines. Serie D: Sciences naturelles 268, 1771-1773 (1969
4. Echalier, G. Drosophila Cells in Culture. (Academic Press, 1997
5. Dobbelaere, J. et al. A genome-wide RNAi screen to dissect centriole duplication and centrosome maturation in Drosophila. PLoS Biol. 6, e224 (2008
6. Mohr, S., Bakal, C. & Perrimon, N. Genomic screening with RNAi: Results and challenges. Annu. Rev. Biochem. 79, 37-64 (2010
7. Castoreno, A.B. et al. Small molecules discovered in a pathway screen target the Rho pathway in cytokinesis. Nat. Chem. Biol. 6, 457-463 (2010
8. Zhang, Y. et al. Expression in aneuploid Drosophila S2 cells. PLoS Biol. 8, e1000320 (2010
9. Gonzalez, M. et al. Generation of stable Drosophila cell lines using multicistronic vectors. Sci. Rep. 1, 75 (2011
10. Simcox, A. et al. Efficient genetic method for establishing Drosophila cell lines unlocks the potential to create lines of specific genotypes. PLoS Genet. 4, e1000142 (2008
11. Donady, J.J. & Seecof, R.L. Effect of the gene lethal (1) myospheroid on Drosophila embryonic cells in vitro. In Vitro 8, 7-12 (1972
12. Prokop, A., Kuppers-Munther, B. & Sanchez-Soriano, N. Using primary neuron cultures of Drosophila to analyze neuronal circuit formation and function. in The Making and Un-making of Neuronal Circuits Ch. 10 (ed. Hassan, B.A.) 225-248 (Humana Press, 2012
13. Grosskortenhaus, R., Pearson, B.J., Marusich, A. & Doe, C.Q. Regulation of temporal identity transitions in Drosophila neuroblasts. Dev. Cell 8, 193-202 (2005
14. Kraft, R. et al. Phenotypes of Drosophila brain neurons in primary culture reveal a role for fascin in neurite shape and trajectory. J. Neurosci. 26, 8734-8747 (2006
15. Kraft, R., Levine, R.B. & Restifo, L.L. The steroid hormone 20-hydroxyecdysone enhances neurite growth of Drosophila mushroom body neurons isolated during metamorphosis. J. Neurosci. 18, 8886-8899 (1998
16. Seecof, R.L., Teplitz, R.L., Gerson, I., Ikeda, K. & Donady, J. Differentiation of neuromuscular junctions in cultures of embryonic Drosophila cells. Proc. Natl. Acad. Sci. USA 69, 566-570 (1972
17. Sicaeros, B., Campusano, J.M. & O'Dowd, D.K. Primary neuronal cultures from the brains of late-stage Drosophila pupae. J. Vis. Exp. 200 (2007
18. Luer, K. & Technau, G.M. Single-cell cultures of Drosophila neuroectodermal and mesectodermal central nervous system progenitors reveal different degrees of developmental autonomy. Neural Develop. 4, 30 (2009
19. Gu, H. et al. Cav2-type calcium channels encoded by cac regulate AP-independent neurotransmitter release at cholinergic synapses in adult Drosophila brain. J. Neurophysiol. 101, 42-53 (2009
20. Su, H. & O'Dowd, D.K. Fast synaptic currents in Drosophila mushroom body Kenyon cells are mediated by α-bungarotoxin-sensitive nicotinic acetylcholine receptors and picrotoxin-sensitive GABA receptors. J. Neurosci. 23, 9246-9253 (2003
21. Campusano, J.M., Su, H., Jiang, S.A., Sicaeros, B. & O'Dowd, D.K. nAChR-mediated calcium responses and plasticity in Drosophila Kenyon cells. Dev. Neurobiol. 67, 1520-1532 (2007
22. Riemensperger, T., Pech, U., Dipt, S. & Fiala, A. Optical calcium imaging in the nervous system of Drosophila melanogaster. Biochim. Biophys. Acta 1820, 1169-1178 (2012
23. Liu, Z., Celotto, A.M., Romero, G., Wipf, P. & Palladino, M.J. Genetically encoded redox sensor identifies the role of ROS in degenerative and mitochondrial disease pathogenesis. Neurobiol. Dis. 45, 362-368 (2012
24. Wu, C.F., Suzuki, N. & Poo, M.M. Dissociated neurons from normal and mutant Drosophila larval central nervous system in cell culture. J. Neurosci. 3, 1888-1899 (1983
25. Ceron, J., Tejedor, F.J. & Moya, F. A primary cell culture of Drosophila postembryonic larval neuroblasts to study cell cycle and asymmetric division. Eur. J. Cell Biol. 85, 567-575 (2006
26. Moraru, M., Egger, B., Bao, D.B. & Sprecher, S.G. Analysis of cell identity, morphology, apoptosis and mitotic activity in a primary neural cell culture system in Drosophila. Neural Develop. 7, 14 (2012
27. Berger, C. et al. FACS purification and transcriptome analysis of Drosophila neural stem cells reveals a role for Klumpfuss in self-renewal. Cell Rep. 2, 407-418 (2012
28. Tian, L. et al. Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators. Nat. Methods 6, 875-881 (2009
29. Ashburner, M., Golic, K.G. & Hawley, R.S. Drosophila: A Laboratory Handbook 2nd edn. (Cold Spring Harbor Laboratory Press, 2005
30. Python, F. & Stocker, R.F. Adult-like complexity of the larval antennal lobe of D. melanogaster despite markedly low numbers of odorant receptor neurons. J. Comparative Neurol. 445, 374-387 (2002
31. Park, J.H. et al. Differential regulation of circadian pacemaker output by separate clock genes in Drosophila. Proc. Natl. Acad. Sci. USA 97, 3608-3613 (2000
32. Poeck, B., Triphan, T., Neuser, K. & Strauss, R. Locomotor control by the central complex in Drosophila - An analysis of the tay bridge mutant. Dev. Neurobiol. 68, 1046-1058 (2008
33. Egger, B., Boone, J.Q., Stevens, N.R., Brand, A.H. & Doe, C.Q. Regulation of spindle orientation and neural stem cell fate in the Drosophila optic lobe. Neural Develop. 2, 1 (2007
34. Prokop, A., Bray, S., Harrison, E. & Technau, G.M. Homeotic regulation of segment-specific differences in neuroblast numbers and proliferation in the Drosophila central nervous system. Mech. Dev. 74, 99-110 (1998
35. Thacker, S.A., Bonnette, P.C. & Duronio, R.J. The contribution of E2F-regulated transcription to Drosophila PCNA gene function. Curr. Biol. 13, 53-58 (2003
36. Langevin, J. et al. Lethal giant larvae controls the localization of notch-signaling regulators numb, neuralized, and Sanpodo in Drosophila sensory-organ precursor cells. Curr. Biol. 15, 955-962 (2005
37. Akerboom, J. et al. Optimization of a GCaMP calcium indicator for neural activity imaging. J. Neurosci. 32, 13819-13840 (2012
38. Weng, M., Komori, H. & Lee, C.Y. Identification of neural stem cells in the Drosophila larval brain. Methods Mol. Biol. 879, 39-46 (2012