Detection and characterization of Shiga toxin-producing Escherichia coli in faeces and lymphatic tissue of free-ranging deer

Epidemiology and Infection

Volumn 141, Issue 2, 2013, Pages 251-259

  Eggert, M ^a   Stuber E ^b   Heurich, M ^c   Fredriksson Ahomaa, M ^d   Burgos, Y ^e   Beutin, L ^e   Martlbauer E ^b  

^a LUDWIG MAXIMILIANS UNIVERSITY   (Germany)

^b UNIVERSITY OF MUNICH   (Germany)

^c BAVARIAN FOREST NATIONAL PARK   (Germany)

^d UNIVERSITY OF HELSINKI   (Finland)

^e FEDERAL INSTITUTE FOR RISK ASSESSMENT   (Germany)

Author keywords

Bacterial typing; Shiga toxin producing E. coli

Indexed keywords

BACTERIAL PROTEIN; SHIGA TOXIN;

ANIMAL TISSUE; ARTICLE; BACTERIAL GENE; BACTERIAL VIRULENCE; BACTERIUM DETECTION; CAPREOLUS CAPREOLUS; CONTROLLED STUDY; DEER; E HLY A GENE; EAE GENE; FECES ANALYSIS; FEMALE; GENETIC ANALYSIS; GERMANY; LYMPHOID TISSUE; MALE; NONHUMAN; PULSED FIELD GEL ELECTROPHORESIS; REAL TIME POLYMERASE CHAIN REACTION; RED DEER; SEROTYPE; SHIGA TOXIN GENE; SHIGA TOXIN PRODUCING ESCHERICHIA COLI; SHIGA TOXIN PRODUCING ESCHERICHIA COLI AUTOAGGLUTINATING ADHESIN GENE;

ANIMALS; DEER; DISEASE RESERVOIRS; ELECTROPHORESIS, GEL, PULSED-FIELD; ESCHERICHIA COLI INFECTIONS; FECES; GERMANY; LYMPHOID TISSUE; REAL-TIME POLYMERASE CHAIN REACTION; SEROTYPING; SHIGA TOXINS; SHIGA-TOXIGENIC ESCHERICHIA COLI; VIRULENCE;

EID: 84876923903     PISSN: 09502688     EISSN: 14694409     Source Type: Journal    
DOI: 10.1017/S0950268812000246     Document Type: Article

References (39)

1. European Food Safety Authority (EFSA). The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2009. EFSA Journal 2011; 9: 2090.
2. Feng PC, et al. Specificity of PCR and serological assays in the detection of Escherichia coli Shiga toxin subtypes. Applied and Environmental Microbiology 2011; 77: 6699-6702.
3. Lefebvre B, et al. Relative cytotoxicity of Escherichia coli O157:H7 isolates from beef cattle and humans. Foodborne Pathogens and Disease 2009; 6: 357-364.
4. Beutin L, et al. Characterization of Shiga toxinproducing Escherichia coli strains isolated from human patients in Germany over a 3-year period. Journal of Clinical Microbiology 2004; 42: 1099-1108. (Pubitemid 38332832)
5. Paton AW, et al. Characterization of Saa, a novel autoagglutinating adhesin produced by locus of enterocyte effacement-negative Shiga-toxigenic Escherichia coli strains that are virulent for humans. Infection and Immunity 2001; 69: 6999-7009. (Pubitemid 32995460)
6. World Health Organisation. Zoonotic non-O157 shiga toxin-producing Escherichia coli (STEC) Report of a WHO Scientific working Group Meeting, 1998, Berlin, 23-26 June 1998.
7. Lim JY, Yoon J, Hovde CJ. A brief overview of Escherichia coli O157:H7 and its plasmid O157. Journal of Microbiology and Biotechnology 2010; 20: 5-14.
8. Bettelheim KA. The non-O157 shiga-toxigenic (verocytotoxigenic) Escherichia coli ; under-rated pathogens. Critical Reviews in Microbiology 2007; 33: 67-87. (Pubitemid 46631085)
9. Pennington H. Escherichia coli O157. Lancet 2010; 376: 1428-1435.
10. Pichner R, et al. Occurrence of Salmonella spp. and shigatoxin-producing Escherichia coli (STEC) in horse faeces and horse meat products [in German]. Berliner und Munchener Tierarztliche Wochenschrift 2005; 118: 321-325. (Pubitemid 41054428)
11. Blanco M, et al. Serotypes, virulence genes, and intimin types of Shiga toxin (verotoxin)-producing Escherichia coli isolates from cattle in Spain and identification of a new intimin variant gene (eae-xi). Journal of Clinical Microbiology 2004; 42: 645-651. (Pubitemid 38222642)
12. Naylor SW, Gally DL, Low JC. Enterohaemorrhagic E. coli in veterinary medicine. International Journal of Medical Microbiology 2005; 295: 419-441. (Pubitemid 41225454)
13. Beutin L, et al. Investigation of human infections with verocytotoxin-producing strains of Escherichia coli (VTEC) belonging to serogroup O118 with evidence for zoonotic transmission. Epidemiology and Infection 2000; 125: 47-54.
14. Sanchez S, et al. Detection and characterisation of Shiga toxin-producing Escherichia coli other than Escherichia coli O157:H7 in wild ruminants. Veterinary Journal 2009; 180: 384-388.
15. Bardiau M, et al. Enteropathogenic (EPEC), enterohaemorragic (EHEC) and verotoxigenic (VTEC) Escherichia coli in wild cervids. Journal of Applied Microbiology 2010; 109: 2214-2222.
16. Kemper N, Aschfalk A, Holler C. Campylobacter spp., Enterococcus spp., Escherichia coli, Salmonella spp., Yersinia spp., and Cryptosporidium oocysts in semidomesticated reindeer (Rangifer tarandus tarandus) in Northern Finland and Norway. Acta Veterinaria Scandinavica 2006; 48: 7.
17. Lehmann S, et al. Detection of STEC in faecal samples of free-ranging wild and in wild meat samples [in German]. Fleischwirtschaft 2006; 4: 93-96.
18. Lillehaug A, et al. Campylobacter spp., Salmonella spp., verocytotoxic Escherichia coli, and antibiotic resistance in indicator organisms in wild cervids. Acta Veterinaria Scandinavica 2005; 46: 23-32. (Pubitemid 43971674)
19. Heurich M, et al. Management and Conservation of large mammals in the Bavarian Forest National Park. Silva Gabreta 2011; 17: 1-18.
20. Sharma VK, Dean-Nystrom EA. Detection of enterohemorrhagic Escherichia coli O157:H7 by using a multiplex real-time PCR assay for genes encoding intimin and Shiga toxins. Veterinary Microbiology 2003; 93: 247-260. (Pubitemid 36412123)
21. Koch C, et al. Isolation of a lysogenic bacteriophage carrying the stx(1(OX3)) gene, which is closely associated with Shiga toxin-producing Escherichia coli strains from sheep and humans. Journal of Clinical Microbiology 2001; 39: 3992-3998. (Pubitemid 33029773)
22. Burk C, et al. Identification and characterization of a new variant of Shiga toxin 1 in Escherichia coli ONT:H19 of bovine origin. Journal of Clinical Microbiology 2003; 41: 2106-2112. (Pubitemid 36576542)
23. Wells CL, Erlandsen SL. Localization of translocating Escherichia coli, Proteus mirabilis, and Enterococcus faecalis within cecal and colonic tissues of monoassociated mice. Infection and Immunity 1991; 59: 4693-4697.
24. Nielsen EM, Andersen MT. Detection and characterization of verocytotoxin-producing Escherichia coli by automated 5k nuclease PCR assay. Journal of Clinical Microbiology 2003; 41: 2884-2893. (Pubitemid 36842275)
25. Beutin L, et al. Identification of human pathogenic strains of shiga toxin-producing Escherichia coli from food by a combination of serotyping and molecular typing of shiga toxin genes. Applied and Environmental Microbiology 2007; 73: 4769-4775. (Pubitemid 47237224)
26. Garcia-Sanchez A, et al. Presence of Shiga toxinproducing E. coli O157:H7 in a survey of wild artiodactyls. Veterinary Microbiology 2007; 121: 373-377. (Pubitemid 46413616)
27. Renter DG, et al. Escherichia coli O157:H7 in freeranging deer in Nebraska. Journal of Wildlife Diseases 2001; 37: 755-760. (Pubitemid 33610046)
28. Leotta GA, et al. Detection and characterization of Shiga toxin-producing Escherichia coli in captive nondomestic mammals. Veterinary Microbiology 2006; 118: 151-157. (Pubitemid 44637258)
29. Asakura H, et al. Detection and genetical characterization of Shiga toxin-producing Escherichia coli from wild deer. Microbiology and Immunology 1998; 42: 815-822. (Pubitemid 29001411)
30. Fukuyama M, et al. Study on the verotoxin-producing Escherichia coli-isolation of the bacteria from deer dung [in Japanese]. Kansenshogaku Zasshi 1999; 73: 1140-1144.
31. Bonardi S, et al. Detection of verocytotoxin-producing Escherichia coli serogroups O157 and O26 in the cecal content and lymphatic tissue of cattle at slaughter in Italy. Journal of Food Protection 2007; 70: 1493-1497. (Pubitemid 46919673)
32. Paulin SM, et al. Analysis of Salmonella enterica serotype-host specificity in calves : avirulence of S. enterica serotype gallinarum correlates with bacterial dissemination from mesenteric lymph nodes and persistence in vivo. Infection and Immunity 2002; 70: 6788-6797. (Pubitemid 35387910)
33. Friedrich AW, et al. Escherichia coli harboring Shiga toxin 2 gene variants : frequency and association with clinical symptoms. Journal of Infectious Diseases 2002; 185: 74-84. (Pubitemid 34043019)
34. Ishii S, Meyer KP, Sadowsky MJ. Relationship between phylogenetic groups, genotypic clusters, and virulence gene profiles of Escherichia coli strains from diverse human and animal sources. Applied and Environmental Microbiology 2007; 73: 5703-5710. (Pubitemid 47462979)
35. Miko A, et al. Assessment of Shiga toxin-producing Escherichia coli isolates from wildlife meat as potential pathogens for humans. Applied and Environmental Microbiology 2009; 75: 6462-6470.
36. Robert Koch-Institut (RKI). SurvStat@RKI database. Surveillance status of EHEC/STEC listed by Serovars reported from 2009-2011 (http://www3.rki. de/SurvStat/QueryForm.aspx). Accessed 15 September 2011.
37. Ahn CK, et al. Deer sausage: a newly identified vehicle of transmission of Escherichia coli O157:H7. Journal of Pediatrics 2009; 155: 587-589.
38. Keene WE, et al. An outbreak of Escherichia coli O157:H7 infections traced to jerky made from deer meat. Journal of the American Medical Association 1997; 277: 1229-1231. (Pubitemid 27172747)
39. Rabatsky-Ehr T, et al. Deer meat as the source for a sporadic case of Escherichia coli O157:H7 infection, Connecticut. Emerging Infectious Diseases 2002; 8: 525-527. (Pubitemid 34533333)