Slowing PCR ramp speed reduces chimera formation from environmental samples

Journal of Microbiological Methods

Volumn 93, Issue 3, 2013, Pages 203-205

  Stevens, Julia L ^a   Jackson, Ronneshia L ^a   Olson, Julie B ^a  

^a UNIVERSITY OF ALABAMA   (United States)

Author keywords

Chimera detection; Chimera formation; Environmental samples; PCR temperatures

Indexed keywords

ANIMAL TISSUE; ARTICLE; CHIMERA; FISH; MICROBIAL COMMUNITY; MICROBIAL DIVERSITY; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SEQUENCE ANALYSIS; SPECIES RICHNESS; TEMPERATURE SENSITIVITY;

ARTIFACTS; ENVIRONMENTAL MICROBIOLOGY; MOLECULAR BIOLOGY; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA; SPECIMEN HANDLING; TEMPERATURE; TIME FACTORS;

EID: 84876833447     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2013.03.013     Document Type: Article

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