Quality control, analysis and secure sharing of Luminex® immunoassay data using the open source LabKey Server platform

BMC Bioinformatics

Volumn 14, Issue , 2013, Pages

  Eckels, Josh ^a   Nathe, Cory ^a   Nelson, Elizabeth K ^a   Shoemaker, Sara G ^b   Nostrand, Elizabeth V ^a   Yates, Nicole L ^c   Ashley, Vicki C ^c   Harris, Linda J ^b   Bollenbeck, Mark ^b   Fong, Youyi ^b   Tomaras, Georgia D ^c   Piehler, Britt ^a  

^a LabKey Software   (United States)

^b Fred Hutchinson Cancer Research Center   (United States)

^c DUKE UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COMMON LANGUAGES; CONTROL INFORMATION; DOSE-RESPONSE CURVES; EXISTING SYSTEMS; GRAPHICAL INTERFACE; INFORMATION CONTENTS; INTEGRATED SOFTWARE; OPEN SOURCE SOFTWARE;

ASSAYS; COMPUTER SOFTWARE; CURVE FITTING; DATA PROCESSING; DISEASES; ELECTRONIC DATA INTERCHANGE; GRAPHICAL USER INTERFACES; METADATA; MULTIPLEXING; OPEN SYSTEMS; QUALITY ASSURANCE; QUALITY CONTROL; TITRATION;

DATA FLOW ANALYSIS;

HUMAN IMMUNODEFICIENCY VIRUS;

ALGORITHM; ARTICLE; COMPUTER PROGRAM; COMPUTER SECURITY; COOPERATION; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS 1; IMMUNOASSAY; IMMUNOLOGY; METHODOLOGY; QUALITY CONTROL; STANDARD;

ALGORITHMS; COMPUTER SECURITY; COOPERATIVE BEHAVIOR; HIV-1; HUMANS; IMMUNOASSAY; QUALITY CONTROL; SOFTWARE;

EID: 84876790151     PISSN: None     EISSN: 14712105     Source Type: Journal    
DOI: 10.1186/1471-2105-14-145     Document Type: Article

References (77)

1. Haynes BF, Gilbert PB, McElrath MJ, Zolla-Pazner S, Tomaras GD, Alam SM, Evans DT, Montefiori DC, Karnasuta C, Sutthent R, Liao H-X, DeVico AL, Lewis GK, Williams C, Pinter A, Fong Y, Janes H, DeCamp A, Huang Y, Rao M, Billings E, Karasavvas N, Robb ML, Ngauy V, De Souza MS, Paris R, Ferrari G, Bailer RT, Soderberg KA, Andrews C, Berman PW, Frahm N, De Rosa SC, Alpert MD, Yates NL, Shen X, Koup RA, Pitisuttithum P, Kaewkungwal J, Nitayaphan S, Rerks-Ngarm S, Michael NL, Kim JH. Immune-Correlates Analysis of an HIV-1 Vaccine Efficacy Trial. N Engl J Med 2012, 366:1275-1286. 10.1056/NEJMoa1113425, 3371689, 22475592.
2. Moody MA, Yates NL, Amos JD, Drinker MS, Eudailey JA, Gurley TC, Marshall DJ, Whitesides JF, Chen X, Foulger A, Yu J-S, Zhang R, Meyerhoff RR, Parks R, Scull JC, Wang L, Vandergrift NA, Pickeral J, Pollara J, Kelsoe G, Alam SM, Ferrari G, Montefiori DC, Voss G, Liao H-X, Tomaras GD, Haynes BF. HIV-1 Gp120 Vaccine Induces Affinity Maturation in Both New and Persistent Antibody Clonal Lineages. J Virol 2012, 86(14):7496-7507. 10.1128/JVI.00426-12, 3416280, 22553329.
3. Churchyard GJ, Morgan C, Adams E, Hural J, Graham BS, Moodie Z, Grove D, Gray G, Bekker L-G, McElrath MJ, Tomaras GD, Goepfert P, Kalams S, Baden LR, Lally M, Dolin R, Blattner W, Kalichman A, Figueroa JP, Pape J, Schechter M, Defawe O, De Rosa SC, Montefiori DC, Nabel GJ, Corey L, Keefer MC. A Phase IIA Randomized Clinical Trial of a Multiclade HIV-1 DNA Prime Followed by a Multiclade rAd5 HIV-1 Vaccine Boost in Healthy Adults (HVTN204). PLoS One 2011, 6(8):e21225. 10.1371/journal.pone.0021225, 3152265, 21857901.
4. Yates NL, Lucas JT, Nolen TL, Vandergrift NA, Soderberg KA, Seaton KE, Denny TN, Haynes BF, Cohen MS, Tomaras GD. Multiple HIV-1-specific IgG3 responses decline during acute HIV-1. AIDS 2011, 25:2089-2097. 10.1097/QAD.0b013e32834b348e, 3667583, 21832938.
5. Tomaras GD, Yates NL, Liu P, Qin L, Fouda GG, Chavez LL, Decamp AC, Parks RJ, Ashley VC, Lucas JT, Cohen M, Eron J, Hicks CB, Liao H-X, Self SG, Landucci G, Forthal DN, Weinhold KJ, Keele BF, Hahn BH, Greenberg ML, Morris L, Karim SSA, Blattner WA, Montefiori DC, Shaw GM, Perelson AS, Haynes BF. Initial B-Cell Responses to Transmitted Human Immunodeficiency Virus Type 1: Virion-Binding Immunoglobulin M (IgM) and IgG Antibodies Followed by Plasma Anti-gp41 Antibodies with Ineffective Control of Initial Viremia. J Virol 2008, 82:12449-12463. 10.1128/JVI.01708-08, 2593361, 18842730.
6. Curtis KA, Kennedy MS, Charurat M, Nasidi A, Delaney K, Spira TJ, Owen SM. Development and Characterization of a Bead-Based, Multiplex Assay for Estimation of Recent HIV Type 1 Infection. AIDS Res Hum Retroviruses 2012, 28:188-197. 10.1089/aid.2011.0037, 21585287.
7. Chowdhury F, Williams A, Johnson P. Validation and comparison of two multiplex technologies, Luminex® and Mesoscale Discovery, for human cytokine profiling. J Immunol Meth 2009, 340:55-64.
8. Elshal MF, McCoy JP. Multiplex bead array assays: Performance evaluation and comparison of sensitivity to ELISA. Methods 2006, 38:317-323. 10.1016/j.ymeth.2005.11.010, 1534009, 16481199.
9. Pine SO, Kublin JG, Hammer SM, Borgerding J, Huang Y, Casimiro DR, McElrath MJ. Pre-Existing Adenovirus Immunity Modifies a Complex Mixed Th1 and Th2 Cytokine Response to an Ad5/HIV-1 Vaccine Candidate in Humans. PLoS One 2011, 6:e18526. 10.1371/journal.pone.0018526, 3076372, 21533229.
10. Opalka D, Lachman CE, MacMullen SA, Jansen KU, Smith JF, Chirmule N, Esser MT. Simultaneous Quantitation of Antibodies to Neutralizing Epitopes on Virus-Like Particles for Human Papillomavirus Types 6, 11, 16, and 18 by a Multiplexed Luminex Assay. Clin Diagn Lab Immunol 2003, 10:108-115. 145272, 12522048.
11. Pickering JW, Martins TB, Greer RW, Schroder MC, Astill ME, Litwin CM, Hildreth SW, Hill HR. A Multiplexed Fluorescent Microsphere Immunoassay for Antibodies to Pneumococcal Capsular Polysaccharides. AJCP 2002, 117:589-596.
12. De Rosa CS, Thomas EP, Bui J, Huang Y, deCamp A, Morgan C, Kalams SA, Tomaras GD, Akondy R, Ahmed R, Lau C-Y, Graham BS, Nabel GJ, McElrath MJ. HIV-DNA Priming Alters T Cell Responses to HIV-Adenovirus Vaccine Even When Responses to DNA Are Undetectable. J Immunol 2011, 187:3391-3401. 10.4049/jimmunol.1101421, 3180898, 21844392.
13. Peiperl L, Morgan C, Moodie Z, Li H, Russell N, Graham BS, Tomaras GD, De Rosa SC, McElrath MJ. The NIAID HIV Vaccine Trials Network: Safety and Immunogenicity of a Replication-Defective Adenovirus Type 5 HIV Vaccine in Ad5-Seronegative Persons: A Randomized Clinical Trial (HVTN 054). PLoS One 2010, 5:e13579. 10.1371/journal.pone.0013579, 2965084, 21048953.
14. De Jager W, Bourcier K, Rijkers GT, Prakken BJ, Seyfert-Margolis V. Prerequisites for cytokine measurements in clinical trials with multiplex immunoassays. BMC Immunol 2009, 10:52. 10.1186/1471-2172-10-52, 2761376, 19785746.
15. Leng SX, McElhaney JE, Walston JD. Xie D, Fedarko NS, Kuchel GA: ELISA and multiplex technologies for cytokine measurement in inflammation and aging research. J Gerontol A Biol Sci Med Sci 2008, 63:879-884. 10.1093/gerona/63.8.879, 2562869, 18772478.
16. Ellington AA, Kullo IJ, Bailey KR, Klee GG. Antibody-Based Protein Multiplex Platforms: Technical and Operational Challenges. Clin Chem 2010, 56:186-193. 10.1373/clinchem.2009.127514, 2901849, 19959625.
17. Breen EC, Reynolds SM, Cox C, Jacobson LP, Magpantay L, Mulder CB, Dibben O, Margolick JB, Bream JH, Sambrano E, Martínez-Maza O, Sinclair E, Borrow P, Landay AL, Rinaldo CR, Norris PJ. Multisite Comparison of High-Sensitivity Multiplex Cytokine Assays. Clin Vaccine Immunol 2011, 18:1229-1242. 10.1128/CVI.05032-11, 3147360, 21697338.
18. Kang J-H, Vanderstichele H, Trojanowski JQ, Shaw LM. Simultaneous analysis of cerebrospinal fluid biomarkers using microsphere-based xMAP multiplex technology for early detection of Alzheimer's disease. Methods 2012, 56:484-493. 10.1016/j.ymeth.2012.03.023, 22503777.
19. xMAP Technology Multiplexing for Bioassays - Luminex Corporation xMAP Technology Multiplexing for Bioassays - Luminex Corporation, [http://www.luminexcorp.com/TechnologiesScience/xMAPTechnology/], xMAP Technology Multiplexing for Bioassays - Luminex Corporation.
20. Bio-Plex 200 Systems (Bio-Rad) [http://www.bio-rad.com/prd/en/US/LSR/PDP/715b85f1-6a4e-41b3-b5d9-80202d779e13/Bio-Plex_200_Systems], Bio-Plex 200 Systems (Bio-Rad).
21. Bio-Plex 3D Suspension Array System (Bio-Rad) [http://www.bio-rad.com/prd/en/US/LSR/PDP/L1AW4B15/Bio-Plex_3D_Suspension_Array_System], Bio-Plex 3D Suspension Array System (Bio-Rad).
22. Millipore -Luminex xMAP Technology, Instrumentation, Software & Templates [http://www.millipore.com/drugdiscovery/dd3/ist], Millipore -Luminex xMAP Technology, Instrumentation, Software & Templates.
23. Multiplex Instruments - Luminex Corporation [http://www.luminexcorp.com/Products/Instruments/], Multiplex Instruments - Luminex Corporation.
24. Meso Scale Discovery (MSD) [http://www.meso-scale.com/catalogsystemweb/webroot/index.aspx], Meso Scale Discovery (MSD).
25. BD Biosciences- Bead-Based Immunoassays [http://www.bdbiosciences.com/reagents/cytometricbeadarray/formats/index.jsp], BD Biosciences- Bead-Based Immunoassays.
26. De Jager W, Rijkers GT. Solid-phase and bead-based cytokine immunoassay: A comparison. Methods 2006, 38:294-303. 10.1016/j.ymeth.2005.11.008, 16481197.
27. Fong Y, Wakefield J, De Rosa S, Frahm N. A Robust Bayesian Random Effects Model for Nonlinear Calibration Problems. Biometrics 2012, 2012:2012. no.
28. Kricka LJ, Master SR. Validation and Quality Control of Protein Microarray-based Analytical Methods. Mol Biotechnol 2007, 38:19-31.
29. Master SR, Bierl C, Kricka LJ. Diagnostic challenges for multiplexed protein microarrays. Drug Discov Today 2006, 11:1007-1011. 10.1016/j.drudis.2006.09.010, 17055410.
30. Martins TB. Development of Internal Controls for the Luminex Instrument as Part of a Multiplex Seven-Analyte Viral Respiratory Antibody Profile. Clin Diagn Lab Immunol 2002, 9:41-45. 119915, 11777827.
31. Rerks-Ngarm S, Pitisuttithum P, Nitayaphan S, Kaewkungwal J, Chiu J, Paris R, Premsri N, Namwat C, De Souza M, Adams E, Benenson M, Gurunathan S, Tartaglia J, McNeil JG, Francis DP, Stablein D, Birx DL, Chunsuttiwat S, Khamboonruang C, Thongcharoen P, Robb ML, Michael NL, Kunasol P, Kim JH. Vaccination with ALVAC and AIDSVAX to Prevent HIV-1 Infection in Thailand. N Engl J Med 2009, 361:2209-2220. 10.1056/NEJMoa0908492, 19843557.
32. Kalos M. An integrative paradigm to impart quality to correlative science. J Transl Med 2010, 8:26. 10.1186/1479-5876-8-26, 2848636, 20233418.
33. Bastarache JA, Koyama T, Wickersham NE, Mitchell DB, Mernaugh RL, Ware LB. Accuracy and reproducibility of a multiplex immunoassay platform: A validation study. J Immunol Meth 2011, 367:33-39.
34. Ellington AA, Kullo IJ, Bailey KR, Klee GG. Measurement and Quality Control Issues in Multiplex Protein Assays: A Case Study. Clin Chem 2009, 55:1092-1099. 10.1373/clinchem.2008.120717, 2965639, 19372187.
35. Huang Y, Gottardo R. Comparability and reproducibility of biomedical data 2012, Brief Bioinform.
36. Sarzotti-Kelsoe M, Cox J, Cleland N, Denny T, Hural J, Needham L, Ozaki D, Rodriguez-Chavez IR, Stevens G, Stiles T, Tarragona-Fiol T, Simkins A. Evaluation and Recommendations on Good Clinical Laboratory Practice Guidelines for Phase I-III Clinical Trials. PLoS Med 2009, 6:e1000067. 10.1371/journal.pmed.1000067, 2670502, 19536325.
37. Britten CM, Janetzki S, Burg SH, Gouttefangeas C, Hoos A. Toward the harmonization of immune monitoring in clinical trials: Quo vadis? Cancer Immunology. Immunotherapy 2007, 57:285-288.
38. Burg SH Van D, Kalos M, Gouttefangeas C, Janetzki S, Ottensmeier C, Welters MJP, Romero P, Britten CM, Hoos A. Harmonization of Immune Biomarker Assays for Clinical Studies. Sci Transl Med 2011, 3:108ps44-108ps44. 10.1126/scitranslmed.3002785, 22072636.
39. Hoos A, Eggermont AMM, Janetzki S, Hodi FS, Ibrahim R, Anderson A, Humphrey R, Blumenstein B, Old L, Wolchok J. Improved Endpoints for Cancer Immunotherapy Trials. J Natl Canc Inst 2010, 102:1388-1397.
40. Rao P, Zhang Z, Gebel H, Bray RA, Guleria I, Mohanakumar T, Nickerson P, Tambur A, Zeevi A, Heeger P, Gjertson D, Reed E. 29-OR: Validation of the Luminex platform to detect and quantify HLA antibodies: Effect of standardizing operation procedures on test variability by CTOT core laboratories. Hum Immunol 2012, 73(25):1-174. 3246109, 22027386.
41. Galon J, Franck P, Marincola FM, Angell HK, Thurin M, Lugli A, Zlobec I, Berger A, Bifulco C, Botti G, Tatangelo F, Britten CM, Kreiter S, Chouchane L, Delrio P, Hartmann A, Asslaber M, Maio M, Masucci GV, Mihm M, Vidal-Vanaclocha F, Allison JP, Gnjatic S, Hakansson L, Huber C, Singh-Jasuja H, Ottensmeier C, Zwierzina H, Laghi L, Grizzi F. Cancer classification using the Immunoscore: a worldwide task force. J Transl Med 2012, 10:205. 10.1186/1479-5876-10-205, 3554496, 23034130.
42. Hoos A, Britten CM, Huber C, O'Donnell-Tormey J. A methodological framework to enhance the clinical success of cancer immunotherapy. Nat Biotechnol 2011, 29:867-870. 10.1038/nbt.2000, 21997622.
43. Taylor CF, Field D, Sansone S-A, Aerts J, Apweiler R, Ashburner M, Ball CA, Binz P-A, Bogue M, Booth T, Brazma A, Brinkman RR, Clark AM, Deutsch EW, Fiehn O, Fostel J, Ghazal P, Gibson F, Gray T, Grimes G, Hancock JM, Hardy NW, Hermjakob H, Julian RK, Kane M, Kettner C, Kinsinger C, Kolker E, Kuiper M, Novère NL, Leebens-Mack J, Lewis SE, Lord P, Mallon A-M, Marthandan N, Masuya H, McNally R, Mehrle A, Morrison N, Orchard S, Quackenbush J, Reecy JM, Robertson DG, Rocca-Serra P, Rodriguez H, Rosenfelder H, Santoyo-Lopez J, Scheuermann RH, Schober D, Smith B, Snape J, Stoeckert CJ, Tipton K, Sterk P, Untergasser A, Vandesompele J, Wiemann S. Promoting coherent minimum reporting guidelines for biological and biomedical investigations: the MIBBI project. Nat Biotechnol 2008, 26:889-896. 10.1038/nbt.1411, 2771753, 18688244.
44. Britten CM, Janetzki S, Burg SH, Huber C, Kalos M, Levitsky HI, Maecker HT, Melief CJM, O'Donnell-Tormey J, Odunsi K, Old LJ, Pawelec G, Roep BO, Romero P, Hoos A, Davis MM. Minimal information about T cell assays: the process of reaching the community of T cell immunologists in cancer and beyond. Canc Immunol Immunother 2010, 60:15-22.
45. Britten CM, Janetzki S, Butterfield LH, Ferrari G, Gouttefangeas C, Huber C, Kalos M, Levitsky HI, Maecker HT, Melief CJM, O'Donnell-Tormey J, Odunsi K, Old LJ, Ottenhoff THM, Ottensmeier C, Pawelec G, Roederer M, Roep BO, Romero P, Van der Burg SH, Walter S, Hoos A, Davis MM. T Cell Assays and MIATA: The Essential Minimum for Maximum Impact. Immunity 2012, 37:1-2. 10.1016/j.immuni.2012.07.010, 22840835.
46. Janetzki S, Britten CM, Kalos M, Levitsky HI, Maecker HT, Melief CJM, Old LJ, Romero P, Hoos A, Davis MM. " MIATA" -Minimal Information about T Cell Assays. Immunity 2009, 31:527-528. 10.1016/j.immuni.2009.09.007, 19833080.
47. Nelson EK, Piehler B, Eckels J, Rauch A, Bellew M, Hussey P, Ramsay S, Nathe C, Lum K, Krouse K, Stearns D, Connolly B, Skillman T, Igra M. LabKey Server: An open source platform for scientific data integration, analysis and collaboration. BMC Bioinformatics 2011, 12:71. 10.1186/1471-2105-12-71, 3062597, 21385461.
48. Rauch A, Bellew M, Eng J, Fitzgibbon M, Holzman T, Hussey P, Igra M, Maclean B, Lin CW, Detter A, Fang R, Faca V, Gafken P, Zhang H, Whiteaker J, Whitaker J, States D, Hanash S, Paulovich A, McIntosh MW. Computational Proteomics Analysis System (CPAS): an extensible, open-source analytic system for evaluating and publishing proteomic data and high throughput biological experiments. J Proteome Res 2006, 5:112-121. 10.1021/pr0503533, 16396501.
49. Levey S, Jennings ER. The use of control charts in the clinical laboratory. Am J Clin Pathol 1950, 20:1059-1066.
50. The Comprehensive R Archive Network [http://cran.r-project.org/], The Comprehensive R Archive Network.
51. Atlas Science Portal [https://atlas.scharp.org/cpas/project/home/begin.view], Atlas Science Portal.
52. Global HIV Vaccine Enterprise [http://www.hivvaccineenterprise.org/], Global HIV Vaccine Enterprise.
53. Coordinating Committee of the Global HIV/AIDS Vaccine Enterprise The Global HIV/AIDS Vaccine Enterprise: Scientific Strategic Plan. PLoS Med 2005, 2:25. Coordinating Committee of the Global HIV/AIDS Vaccine Enterprise.
54. LabKey Server Tool for Luminex® Assays - Tutorial Level I [https://www.labkey.org/wiki/home/Documentation/page.view?name=luminexAssayTutorial], LabKey Server Tool for Luminex® Assays - Tutorial Level I.
55. LabKey Server Tool for Luminex® Assays - Tutorial Level II [https://www.labkey.org/wiki/home/Documentation/page.view?name=advancedLuminexTutorial], LabKey Server Tool for Luminex® Assays - Tutorial Level II.
56. Troubleshooting guide for Luminex transform scripts and curve fits [https://www.labkey.org/wiki/home/Documentation/page.view?name=troubleshootLuminexTransformScripts], Troubleshooting guide for Luminex transform scripts and curve fits.
57. LabKey Server Tool for Luminex® Assays - Tutorial Live Demo [https://www.labkey.org/project/home/Study/Luminex/begin.view?], LabKey Server Tool for Luminex® Assays - Tutorial Live Demo.
58. Bio-Plex® Assay Data Analysis: Applications & Technologies (Bio-Rad) [http://www.bio-rad.com/evportal/en/US/LSR/Solutions/LUSM33IVK/Bio-Plex%C2%AE_Assay_Data_Analysis], Bio-Plex® Assay Data Analysis: Applications & Technologies (Bio-Rad).
59. Piehler B, Nelson EK, Eckels J, Ramsay S, Lum K, Wood B, Greene KM, Gao H, Seaman MS, Montefiori DC, Igra M. LabKey Server NAb: A tool for analyzing, visualizing and sharing results from neutralizing antibody assays. BMC Immunol 2011, 12:33. 10.1186/1471-2172-12-33, 3115917, 21619655.
60. Iversen PW, Beck B, Chen Y-F, Dere W, Devanarayan V, Eastwood BJ, Farmen MW, Iturria SJ, Montrose C, Moore RA, Weidner JR, Sittampalam GS. HTS Assay Validation. In Assay Guidance Manual 2012, Eli Lilly & Company and the National Center for Advancing Translational Sciences: Bethesda (MD).
61. Beck B, Chen Y-F, Dere W, Devanarayan V, Eastwood BJ, Farmen MW, Iturria SJ, Iversen PW, Kahl SD, Moore RA, Sawyer BD, Weidner J. Assay Operations for SAR Support. In Assay Guidance Manual 2012, Eli Lilly & Company and the National Center for Advancing Translational Sciences: Bethesda (MD).
62. Ritz C, Streibig JC. Bioassay Analysis using R. J Stat Software 2005, 12:1-22.
63. Gottschalk PG, Dunn JR. The five-parameter logistic: a characterization and comparison with the four-parameter logistic. Anal Biochem 2005, 343:54-65. 10.1016/j.ab.2005.04.035, 15953581.
64. Motulsky H, Christopoulos A. Fitting Models to 2004, Biological Data Using Linear and Nonlinear Regression: A Practical Guide to Curve Fitting. Oxford University Press.
65. Shulman N, Bellew M, Snelling G, Carter D, Huang Y, Li H, Self SG, McElrath MJ, De Rosa SC. Development of an automated analysis system for data from flow cytometric intracellular cytokine staining assays from clinical vaccine trials. Cytometry 2008, 73A:847-856.
66. Bellew M, Krouse K, Snelling G, Treister A, Self SG, McElrath MJ, De Rosa SC. Repository for data and analyses from multiparameter flow cytometric assays. J Med Primatol 2009, 38:67-93.
67. Eckels J, Hussey P, Nelson EK, Myers T, Rauch A, Bellew M, Connolly B, Law W, Eng JK, Katz J, McIntosh M, Mallick P, Igra M. Installation and Use of LabKey Server for Proteomics 2002, John Wiley & Sons, Inc: In Current Protocols in Bioinformatics.
68. Burwitz BJ, Sacha JB, Reed JS, Newman LP, Norante FA, Bimber BN, Wilson NA, Watkins DI, O'Connor DH. Pyrosequencing reveals restricted patterns of CD8+ T cell escape-associated compensatory mutations in simian immunodeficiency virus. J Virol 2011, 85:13088-13096. 10.1128/JVI.05650-11, 3233179, 21994463.
69. Moreland AJ, Guethlein LA, Reeves RK, Broman KW, Johnson RP, Parham P, O'Connor DH, Bimber BN. Characterization of killer immunoglobulin-like receptor genetics and comprehensive genotyping by pyrosequencing in rhesus macaques. BMC Genom 2011, 12:295.
70. Apache License, Version 2.0 [http://www.apache.org/licenses/LICENSE-2.0.html], Apache License, Version 2.0.
71. Luminex Corporation [http://www.luminexcorp.com/], Luminex Corporation.
72. MiraiBio - MasterPlex QT for Multiplex Quantitative Analysis Curve-Fitting for Luminex 100/Luminex 200/MAGPIX/Bio-Plex/MSD Platforms [http://www.miraibio.com/masterplex-qt/qt-luminex-quantitative-data-analysis-software.html], MiraiBio - MasterPlex QT for Multiplex Quantitative Analysis Curve-Fitting for Luminex 100/Luminex 200/MAGPIX/Bio-Plex/MSD Platforms.
73. GraphPad Prism: Scientific graphing, curve fitting and statistics [http://www.graphpad.com/prism/prism.htm], GraphPad Prism: Scientific graphing, curve fitting and statistics.
74. Microsoft Excel [http://office.microsoft.com/en-us/excel/], Microsoft Excel.
75. The R Project for Statistical Computing [http://www.r-project.org/], The R Project for Statistical Computing.
76. TrialShare Portal - Immune Tolerance Network [https://itntrialshare.org/], TrialShare Portal - Immune Tolerance Network.
77. Nelson EK, Piehler B, Rauch A, Ramsay S, Holman D, Asare S, Asare A, Igra M. Ancillary study management systems: a review of needs. BMC Med Inform Decis Mak 2013, 13:5. 10.1186/1472-6947-13-5, 3564696, 23294514.