Diced electrophoresis gel assay for screening enzymes with specified activities

Journal of the American Chemical Society

Volumn 135, Issue 16, 2013, Pages 6002-6005

  Komatsu, Toru ^a   Hanaoka, Kenjiro ^a   Adibekian, Alexander ^b   Yoshioka, Kentaro ^a   Terai, Takuya ^a   Ueno, Tasuku ^a   Kawaguchi, Mitsuyasu ^a   Cravatt, Benjamin F ^b   Nagano, Tetsuo ^a  

^a UNIVERSITY OF TOKYO   (Japan)

^b SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHEMOATTRACTANTS; FLUOROMETRIC ASSAY; METABOLIC INACTIVATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN SPOTS; SCREENING TOOL;

ELECTROPHORESIS; FLUORESCENCE;

ENZYMES;

ARTICLE; CONTROLLED STUDY; DICED ELECTROPHORESIS GEL ASSAY; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME METABOLISM; ENZYME SPECIFICITY; FLUORESCENCE; FLUOROMETRY; GEL ELECTROPHORESIS; METABOLIC INACTIVATION; MOLECULAR PROBE; MULTIWELL PLATE BASED FLUOROMETRIC ASSAY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ANALYSIS; PROTEIN FUNCTION; PROTEIN METABOLISM; PROTEIN STRUCTURE;

ACRYLIC RESINS; ANIMALS; CHEMOTACTIC FACTORS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYMES; ESCHERICHIA COLI; ESTERASES; FLUORESCENT DYES; FLUOROMETRY; GLUCOSIDASES; HIGH-THROUGHPUT SCREENING ASSAYS; ISOELECTRIC FOCUSING; LIVER; MASS SPECTROMETRY; MICE; MOLECULAR MIMICRY; NEUTROPHILS; PEPTIDE HYDROLASES; PROTEINS;

EID: 84876721515     PISSN: 00027863     EISSN: 15205126     Source Type: Journal    
DOI: 10.1021/ja401792d     Document Type: Article

References (21)

1. Kubinyi, H. Nat. Rev. Drug Discovery 2003, 2, 665
2. Saghatelian, A.; Cravatt, B. F. Nat. Chem. Biol. 2005, 1, 130
3. Drucker, D.; Easley, C.; Kirkpatrick, P. Nat. Rev. Drug. Discovery 2007, 6, 109
4. Suda, H.; Yamamoto, K.; Aoyagi, T.; Umezawa, H. Biochim. Biophys. Acta 1980, 616, 60
5. Manchenko, G. P. Handbook of Detection of Enzymes on Electrophoretic Gels, 2 nd ed.; CRC Press: Boca Raton, FL, 2003.
6. Wittig, I.; Braun, H. P.; Schagger, H. Nat. Protoc. 2006, 1, 418
7. Urano, Y.; Kamiya, M.; Kanda, K.; Ueno, T.; Hirose, K.; Nagano, T. J. Am. Chem. Soc. 2005, 127, 4888
8. Lomolino, G.; Lante, A.; Crapisi, A.; Spettoli, P.; Curioni, A. Electrophoresis 2001, 22, 1021
9. Zhang, Q.; Raoof, M.; Chen, Y.; Sumi, Y.; Sursal, T.; Junger, W.; Brohi, K.; Itagaki, K.; Hauser, C. J. Nature 2010, 464, 104
10. Marasco, W. A.; Phan, S. H.; Krutzsch, H.; Showell, H. J.; Feltner, D. E.; Nairn, R.; Becker, E. L.; Ward, P. A. J. Biol. Chem. 1984, 259, 5430
11. Pike, M. C.; Fischer, D. G.; Koren, H. S.; Snyderman, R. J. Exp. Med. 1980, 152, 31
12. Orning, L.; Fitzpatrick, F. A. Biochemistry 1992, 31, 4218
13. Bauvois, B.; Dauzonne, D. Med. Res. Rev. 2006, 26, 88
14. Backes, B. J.; Harris, J. L.; Leonetti, F.; Craik, C. S.; Ellman, J. A. Nat. Biotechnol. 2000, 18, 187
15. Adibekian, A.; Martin, B. R.; Wang, C.; Hsu, K. L.; Bachovchin, D. A.; Niessen, S.; Hoover, H.; Cravatt, B. F. Nat. Chem. Biol. 2011, 7, 469
16. Sandomenico, A.; Russo, A.; Palmieri, G.; Bergamo, P.; Gogliettino, M.; Falcigno, L.; Ruvo, M. J. Med. Chem. 2012, 55, 2102
17. Scaloni, A.; Jones, W. M.; Barra, D.; Pospischil, M.; Sassa, S.; Popowicz, A.; Manning, L. R.; Schneewind, O.; Manning, J. M. J. Biol. Chem. 1992, 267, 3811
18. Berkers, C. R.; Verdoes, M.; Lichtman, E.; Fiebiger, E.; Kessler, B. M.; Anderson, K. C.; Ploegh, H. L.; Ovaa, H.; Galardy, P. J. Nat. Methods 2005, 2, 357
19. Scaloni, A.; Jones, W.; Pospischil, M.; Sassa, S.; Schneewind, O.; Popowicz, A. M.; Bossa, F.; Graziano, S. L.; Manning, J. M. J. Lab. Clin. Med. 1992, 120, 546
20. Shimizu, K.; Ikegami-Kawai, M.; Takahashi, T. Biol. Pharm. Bull. 2009, 32, 1632
21. Yamin, R.; Bagchi, S.; Hildebrant, R.; Scaloni, A.; Widom, R. L.; Abraham, C. R. J. Neurochem. 2007, 100, 458