Effect of VIP on intracellular [Ca2+], extracellular regulated kinase 1/2, and secretion in cultured rat conjunctival goblet cells

Investigative Ophthalmology and Visual Science

Volumn 54, Issue 4, 2013, Pages 2872-2884

  Li, Dayu ^a   Jiao, Jianwei ^a   Shatos, Marie A ^a   Hodges, Robin R ^a   Dartt, Darlene A ^a  

^a SCHEPENS EYE RESEARCH INSTITUTE   (United States)

Author keywords

Conjunctiva; ERK; Goblet cells; Intracellular Ca2+

Indexed keywords

EGTAZIC ACID; GLYCOCONJUGATE; MITOGEN ACTIVATED PROTEIN KINASE 1; MITOGEN ACTIVATED PROTEIN KINASE 3; MUCIN 5AC; N [2 (4 BROMOCINNAMYLAMINO) ETHYL] 5 ISOQUINOLINESULFONAMIDE; SMALL INTERFERING RNA; UNCLASSIFIED DRUG; VASOACTIVE INTESTINAL POLYPEPTIDE; VASOACTIVE INTESTINAL POLYPEPTIDE RECEPTOR 1; VASOACTIVE INTESTINAL POLYPEPTIDE RECEPTOR 2;

ANIMAL CELL; ARTICLE; CALCIUM CELL LEVEL; CALCIUM TRANSPORT; CHELATION; CONJUNCTIVA EPITHELIUM; GOBLET CELL; IMMUNOFLUORESCENCE; IMMUNOPRECIPITATION; MALE; NONHUMAN; PRIORITY JOURNAL; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SIGNAL TRANSDUCTION; WESTERN BLOTTING;

ANIMALS; CALCIUM; CELLS, CULTURED; CONJUNCTIVA; CYCLIC AMP-DEPENDENT PROTEIN KINASES; DNA, COMPLEMENTARY; GLYCOCONJUGATES; GOBLET CELLS; MALE; MAP KINASE SIGNALING SYSTEM; MITOGEN-ACTIVATED PROTEIN KINASE 1; MITOGEN-ACTIVATED PROTEIN KINASE 3; MUCIN 5AC; ORGAN CULTURE TECHNIQUES; PARASYMPATHETIC NERVOUS SYSTEM; RATS; RATS, SPRAGUE-DAWLEY; RECEPTORS, VASOACTIVE INTESTINAL PEPTIDE, TYPE II; RECEPTORS, VASOACTIVE INTESTINAL POLYPEPTIDE, TYPE I; VASOACTIVE INTESTINAL PEPTIDE;

EID: 84876575825     PISSN: 01460404     EISSN: 15525783     Source Type: Journal    
DOI: 10.1167/iovs.12-11264     Document Type: Article

References (32)

1. Rios JD, Zoukhri D, Rawe IM, et al. Immunolocalization of muscarinic and VIP receptor subtypes and their role in stimulating goblet cell secretion. Invest Ophthalmol Vis Sci. 1999;40:1102-1111.
2. Horikawa Y, Shatos MA, Hodges RR, et al. Activation of mitogen-activated protein kinase by cholinergic agonists and EGF in human compared with rat cultured conjunctival goblet cells. Invest Ophthalmol Vis Sci. 2003;44:2535-2544.
3. Hodges RR, Bair JA, Carozza RB, et al. Signaling pathways used by EGF to stimulate conjunctival goblet cell secretion. Exp Eye Res. 2012;103:99-113.
4. Kanno H, Horikawa Y, Hodges RR, et al. Cholinergic agonists transactivate EGFR and stimulate MAPK to induce goblet cell secretion. Am J Physiol Cell Physiol. 2003;284:C988-C998.
5. Dartt DA, Baker AK, Vaillant C, et al. Vasoactive intestinal polypeptide stimulation of protein secretion from rat lacrimal gland acini. Am J Physiol. 1984;247(5 pt 1):G502-G509.
6. Hallden G, Abens J, Engstrom C, et al. Iodinated derivatives of the vasoactive intestinal polypeptide (VIP) are agonists at the cat pancreas and the rat submandibular salivary gland. Regul Pept. 1986;16:183-188.
7. Scott J, Baum BJ. Involvement of cyclic AMP and calcium in exocrine protein secretion induced by vasoactive intestinal polypeptide in rat parotid cells. Biochim Biophys Acta. 1985; 847:255-262.
8. Turner JT, Camden JM. Regulation of secretion by vasoactiveintestinal peptide in isolated perfused rat submandibular glands. Arch Oral Biol. 1992;37:281-287.
9. McCool DJ, Marcon MA, Forstner JF, et al. The T84 human colonic adenocarcinoma cell line produces mucin in culture and releases it in response to various secretagogues. Biochem J. 1990;267:491-500.
10. Kamijo A, Terakawa S, Hisamatsu K. Neurotransmitter-induced exocytosis in goblet and acinar cells of rat nasal mucosa studied by video microscopy. Am J Physiol. 1993;265(2 pt 1): L200-L209.
11. Wilkins TJ, de Ondarza J, Hootman SR. Intracellular mediators of goblet cell degranulation in isolated pancreatic ducts. Biochem Biophys Res Commun. 1994;205:423-428.
12. Lazarus SC, Basbaum CB, Barnes PJ, et al. cAMP immunocy-tochemistry provides evidence for functional VIP receptors in trachea. Am J Physiol. 1986;251(1 pt 1):C115-C119.
13. Dartt DA, Kessler TL, Chung EH, et al. Vasoactive intestinal peptide-stimulated glycoconjugate secretion from conjunctival goblet cells. Exp Eye Res. 1996;63:27-34.
14. Shatos MA, Rios JD, Tepavcevic V, et al. Isolation, characterization, and propagation of rat conjunctival goblet cells in vitro. Invest Ophthalmol Vis Sci. 2001;42:1455-1464.
15. Billington CK, Hall IP. Novel cAMP signalling paradigms: therapeutic implications for airway disease. Br J Pharmacol. 2012;166:401-410.
16. Holz GG, Kang G, Harbeck M, et al. Cell physiology of cAMP sensor Epac. J Physiol. 2006;577(pt 1):5-15.
17. Wojcikiewicz RJ, Luo SG. Phosphorylation of inositol 1,4,5- trisphosphate receptors by cAMP-dependent protein kinase. Type I, II, and III receptors are differentially susceptible to phosphorylation and are phosphorylated in intact cells. J Biol Chem. 1998;273:5670-5677.
18. Lee RJ, Foskett JK. cAMP-activated Ca2+ signaling is required for CFTR-mediated serous cell fluid secretion in porcine and human airways. J Clin Invest. 2010;120:3137-3148.
19. Shatos MA, Rios JD, Horikawa Y, et al. Isolation and characterization of cultured human conjunctival goblet cells. Invest Ophthalmol Vis Sci. 2003;44:2477-2486.
20. Castorina A, Tiralongo A, Giunta S, et al. PACAP and VIP prevent apoptosis in schwannoma cells. Brain Res. 2008; 1241:29-35.
21. Dartt DA, Hodges RR, Li D, et al. Conjunctival goblet cell secretion stimulated by leukotrienes is reduced by resolvins D1 and E1 to promote resolution of inflammation. J Immunol. 2011;186:4455-4466.
22. Shatos MA, Hodges RR, Bair JA, et al. Stimulatory role of PKCalpha in extracellular regulated kinase 1/2 pathway in conjunctival goblet cell proliferation. Invest Ophthalmol Vis Sci. 2009;50:1619-1625.
23. Shatos MA, Hodges RR, Oshi Y, et al. Role of cPKCalpha and nPKCepsilon in EGF-stimulated goblet cell proliferation. Invest Ophthalmol Vis Sci. 2009;50:614-620.
24. Hayashi D, Li D, Hayashi C, et al. Role of histamine and its receptor subtypes in stimulation of conjunctival goblet cell secretion. Invest Ophthalmol Vis Sci. 2012;53:2993-3003.
25. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72:248-254.
26. Bugrim AE. Regulation of Ca2+ release by cAMP-dependent protein kinase. A mechanism for agonist-specific calcium signaling? Cell Calcium. 1999;25:219-226.
27. DeSouza N, Reiken S, Ondrias K, et al. Protein kinase A and two phosphatases are components of the inositol 1,4,5-trisphosphate receptor macromolecular signaling complex. J Biol Chem. 2002;277:39397-39400.
28. Taylor CW, Tovey SC. From parathyroid hormone to cytosolic Ca2+ signals. Biochem Soc Trans. 2012;40:47-152.
29. Mayati A, Levoin N, Paris H, et al. Induction of intracellular calcium concentration by environmental benzo(a)pyrene involves a beta2-adrenergic receptor/adenylyl cyclase/Epac-1/ inositol 1,4,5-trisphosphate pathway in endothelial cells. J Biol Chem. 2012;287:4041-4052.
30. Shah AU, Grant WM, Latif SU, et al. Cyclic AMP accelerates calcium waves in pancreatic acinar cells. Am J Physiol Gastrointest Liver Physiol. 2008;294:G1328-G1334.
31. Pereira L, Metrich M, Fernandez-Velasco M, et al. The cAMP binding protein Epac modulates Ca2+ sparks by a Ca2+/ calmodulin kinase signalling pathway in rat cardiac myocytes. J Physiol. 2007;583(PART 2):685-694.
32. Yeh CK, Ghosh PM, Dang H, et al. beta-Adrenergic-responsive activation of extracellular signal-regulated protein kinases in salivary cells: role of epidermal growth factor receptor and cAMP. Am J Physiol Cell Physiol. 2005;288:C1357-C1366.