Adaptation of stem cells to 96-well plate assays: Use of human embryonic and mouse neural stem cells in the MTT assay

Current Protocols in Stem Cell Biology

Volumn 1, Issue SUPPL.23, 2013, Pages

  Behar, Rachel Z ^a   Bahl, Vasundhra ^a   Wang, Yuhuan ^a   Weng, Jo Hao ^a   Lin, Sabrina C ^a   Talbot, Prue ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

96 well plate assays; Human embryonic stem cells; Mouse neural stem cells; MTT assay; Toxicology

Indexed keywords

4 (1 AMINOETHYL) N (4 PYRIDYL)CYCLOHEXANECARBOXAMIDE; PHOSPHATE BUFFERED SALINE; RHO KINASE INHIBITOR; TETRAZOLIUM; THIAZOLE DERIVATIVE; THIAZOLYL BLUE;

ACCURACY; ADAPTATION; ARTICLE; CELL ASSAY; CELL COUNT; CELL CULTURE; CELL PROLIFERATION; CELL SURVIVAL; CELL SUSPENSION; CELL VIABILITY; CENTRIFUGATION; CULTURE MEDIUM; CYTOTOXICITY; EMBRYONIC STEM CELL; HUMAN; HUMIDITY; IC 50; NEURAL STEM CELL; PH; PRIORITY JOURNAL; REPRODUCIBILITY; ROOM TEMPERATURE; SCREENING; SPECTROPHOTOMETRY; ANIMAL CELL; CELL DEATH; CONCENTRATION RESPONSE; HUMAN CELL; INCUBATION TIME; MICROPLATE READER; NONHUMAN; SPECTROPHOTOMETER; TISSUE CULTURE; ANIMAL; CYTOLOGY; METHODOLOGY; MOUSE; STEM CELL; STEM CELL TRANSPLANTATION;

ANIMALS; CELL COUNT; EMBRYONIC STEM CELLS; HUMANS; MICE; NEURAL STEM CELLS; SPECTROPHOTOMETRY; STEM CELL TRANSPLANTATION; STEM CELLS; TETRAZOLIUM SALTS; THIAZOLES;

EID: 84876497891     PISSN: 19417322     EISSN: 19388969     Source Type: Journal    
DOI: 10.1002/9780470151808.sc01c13s23     Document Type: Article

References (12)

1. Berridge,M.V., Tan, A.S.,McCoy,K.D., andWang, R. 1996. The biochemical and cellular basis of cell proliferation assays that use tetrazolium salts. Biochemica 4:14-19.
2. Blein, O., Ronot, X., and Adolphe, M. 1991. Cross contamination associated with the use of multiwell culture plates for cytotoxicity assessment of volatile chemicals. Cytotechnology 6:79-82.
3. Freshney, R.I. 2006. Basic principles of cell culture. In Culture of Cells for Tissue Engineering (R.I. Freshney and G. Vunjak-Novakovic, eds.) pp. 3-22. John Wiley & Sons, Hoboken, N.J. Fujimura, M.,Usuki, F., Kawamura, M., and Izumo, S. 2011. Inhibition of the Rho/ROCK pathway prevents neuronal degeneration in vitro and in vivo following methylmercury exposure. Toxicol. Appl. Pharmacol. 250:1-9.
4. Fujimura, M.,Usuki, F., Kawamura, M., and Izumo, S. 2011. Inhibition of the Rho/ROCK pathway prevents neuronal degeneration in vitro and in vivo following methylmercury exposure. Toxicol. Appl. Pharmacol. 250:1-9.
5. Grandjean, P., Bellinger, D., Bergman, A., Cordier, S., Davey-Smith, G., Eskenazi, B., Gee, D., Gray, K., Hanson, M., van den Hazel, P., Heindel, J.J., Heinzow, B., Hertz-Picciotto, I., Hu, H., Huang, T.T., Jensen, T.K., Landrigan, P.J., McMillen, I.C., Murata, K., Ritz, B., Schoeters, G., Skakkebaek, N.E., Skerfving, S., andWeihe, P. 2008. The faroes statement: Human health effects of developmental exposure to chemicals in our environment. Basic Clin. Pharmacol. Toxicol. 102:73-75.
6. Lin, S. and Talbot, P. 2011. Methods for culturing mouse and human embryonic stem cells. Methods Mol. Biol. 690:31-56.
7. Lin, S., Fonteno, S., Weng, J.H., and Talbot, P. 2010. Comparison of the toxicity of smoke from conventional and harm reduction cigarettes using human embryonic stem cells. Toxicol. Sci. 118:202-212.
8. Mossman, B.T. 1983. In vitro approaches for determining mechanisms of toxicity and carcinogenicity by asbestos in the gastrointestinal and respiratory tracts. Environ. Health Perspect. 53:155-161.
9. Ohgushi, M., Matsumura, M., Eiraku, M., Murakami, K., Aramaki, T., Nishiyama, A., Muguruma, K., Nakano, T., Suga, H., Ueno, M., Ishizaki, T., Suemori, H., Narumiya, S., Niwa, H., and Sasai, Y. 2010. Molecular pathway and cell state responsible for dissociation-induced apoptosis in human pluripotent stem cells. Cell Stem Cell 7:225-239.
10. Parker, M.A., Anderson, J.K., Corliss, D.A., Abraria, V.E., Sidman, R.L., Park, K.I., Teng, Y.D., Cotanche, D.A., and Snyder, E.Y. 2005. Expression profile of an operationally-defined neural stem cell clone. Exp. Neurol. 194:320- 332.
11. Talbot, P. and Lin, S. 2011. Mouse and human embryonic stem cells: Can they improve human health by preventing disease. Curr. Top. Med. Chem. 11:1638-1652.
12. Watanabe, K., Ueno, M., Kamiya, D., Nishiyama, A.,Matsumura, M.,Wataya, T., Takahashi, J.B., Nishikawa, S., Muguruma, K., and Sasai, Y. 2007. A ROCK inhibitor permits survival of dissociated human embryonic stem cells. Nat. Biotechnol. 25:681-686.