Fixation/Permeabilization: New alternative procedure for immunofluorescence and mrna in situ hybridization of vertebrate and invertebrate embryos

Developmental Dynamics

Volumn 242, Issue 5, 2013, Pages 503-517

  Fernández, Juan ^a   Fuentes, Ricardo ^a  

^a UNIVERSITY OF CHILE   (Chile)

Author keywords

Fixation permeabilization; Immunofluorescence; Invertebrate embryos; MRNA in situ hybridization

Indexed keywords

CARBOXYLIC ACID; MESSENGER RNA; PROTEINASE;

ANIMAL CELL; ARTICLE; CELL PERMEABILIZATION; CHICK; CONTROLLED STUDY; CYTOPLASM; CYTOSKELETON; DROSOPHILA; EMBRYO; IMMUNOFLUORESCENCE; IN SITU HYBRIDIZATION; INVERTEBRATE; LEECH; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; VERTEBRATE; ZEBRA FISH; ZYGOTE;

ANIMALS; CHICK EMBRYO; DROSOPHILA; EMBRYO, MAMMALIAN; FLUORESCENT ANTIBODY TECHNIQUE; IN SITU HYBRIDIZATION; LEECHES; PERMEABILITY; RNA, MESSENGER; SPECIMEN HANDLING; STAINING AND LABELING; TISSUE FIXATION; ZEBRAFISH;

DANIO RERIO; HIRUDINIDA; INVERTEBRATA; VERTEBRATA;

EID: 84876488851     PISSN: 10588388     EISSN: 10970177     Source Type: Journal    
DOI: 10.1002/dvdy.23943     Document Type: Article

References (69)

1. Allende ML, Amsterdam A, Becker T, Kawakami K, Gaiano N, Hopkins N. 1996. Insertional mutagenesis in zebrafish identifies two novel genes, pescadillo and dead eye, essential for embryonic development. Genes Dev 10:3141-3155.
2. Baker JR. 1950. Cytological technique. 3rd ed. London: Methuen & Co. Ltd. 211 p.
3. Cantillana V, Urrutia M, Ubilla A, Fernández J. 2000. The complex dynamic network of microtubule and microfilament cytasters of the leech zygote. Dev Biol 228:136-149.
4. Dedon PC, Soults JA, Allis D, Gorovsky MA. 1991. Formaldehyde cross-linking and immunoprecipitation demonstrate developmental changes in H1 association with transcriptionally active genes. Mol Cell Biol 11:1729-1733.
5. Dekens MPS, Pelegri F, Maischein HM, Nüsslein-Volhard C. 2003. The maternal-effect gene futile cycle is essential for pronuclear congression and mitotic spindle assembly in the zebrafish zygote. Development 130:3907-3916.
6. Fernández J. 1980. Embryonic development of the glossiphoniid leech Theromyzon rude: characterization of developmental stages. Dev Biol 78:407-434.
7. Fernández J, Olea N. 1982. Embryonic development of glossiphoniid leeches. In: Harrison FW, Cowden RR, editors. Developmental biology of freshwater invertebrates. New York: AR Liss. p 317-366.
8. Fernández J, Olea N. 1995. Formation of the female pronucleus and reorganization and disassembly of the first interphase cytoskeleton in the egg of the glossiphoniid leech Theromyzon rude. Dev Biol 171:541-553.
9. Fernández J, Olea N, Tellez V, Matte C. 1990. Structure and development of the egg of the glossiphoniid leech Theromyzon rude: reorganization of the fertilized egg during completion of the first meiotic division. Dev Biol 137:142-154.
10. Fernández J, Olea N, Tellez V. 1994. Formation of the male pronucleus, organization of the first interphase monaster and establishment of the perinuclear plasm domain in the egg of the glossiphoniid leech Theromyzon rude. Dev Biol 164:111-122.
11. Fernández J, Olea N, Ubilla A, Cantillana V. 1998. Formation of polar cytoplasmic domains (teloplasms) in the leech egg is a three-step segregation process. Intl J Dev Biol 42:149-162.
12. Fernández J, Valladares M, Fuentes R, Ubilla A. 2006. Reorganization of cytoplasm in the zebrafish oocyte and egg during early steps of ooplasmic segregation. Dev Dyn 235:656-671.
13. Frasch M, Hoey T, Rushlow C, Doyle H, Levine M. 1987. Characterization and localization of the even-skipped proteins of Drosophila. EMBO J 6:749-759.
14. Fuentes R, Fernández J. 2010. Ooplasmic segregation in the zebrafish zygote and early embryo: pattern of ooplasmic movements and transport pathways. Dev Dyn 239:2172-2189.
15. Gard DL. 1991. Organization, nucleation, and acetylation of microtubules in Xenopus laevis oocytes: a study by confocal immunofluorescence microscopy. Dev Biol 143:346-362.
16. Hammati-Brivenlou A, Harland RM. 1989. Expression of an engrailed-related protein is induced in the anterior neural ectoderm of early Xenopus embryos. Development 106:611-617.
17. Harland RM. 1991. In situ hybridization: an improved whole-mount method for Xenopus embryos. In: Kay BK, Peng HB, editors. Methods in cell biology. San Diego: Academic Press Inc. p 685-695.
18. Hashimoto Y, Maegawa S, Nagai T, Yamaha E, Suzuki H, Yasuda K, Inoue K. 2004. Localized maternal factors are required for zebrafish germ cell formation. Dev Biol 268:152-161.
19. Hayat MA. 2002. Microscopy, immunohistochemistry and antigen retrieval methods. For light and electron microscopy. New York: Kluwer Academic/Plenum Publishers. 359 p.
20. Henrique D, Adam J, Myat A, Chitnis A, Lewis J, Ish-Horowics D. 1995. Expression of a Delta homologue in prospective neurons in the chick. Nature 375:787-790.
21. Hoppert M. 2003. Microscopic techniques in biotechnology. Weinheim: Willey VCH Verlag. GmbH&Co KGaA. 342 p.
22. Inoue D, Wittbrodt J. 2011. One for all-A highly efficient and versatile method for fluorescent immunostaining in fish embryos. PloS ONE 6:e19713.
23. Jesuthasan S. 1998. Furrow-associated microtubule arrays are required for the cohesion of zebrafish blastomeres following cytokinesis. J Cell Sci 111:3695-3703.
24. Kang D, Pillon M, Weisblat DA. 2002. Maternal and zygotic expression of a nanos-class gene in the leech Helobdella robusta: primordial germ cells arise from segmental mesoderm. Dev Biol 245:28-41.
25. Karr TL, Alberts BM. 1986. Organization of the cytoskeleton in early Drosophila embryos. J Cell Biol 102:1494-1509.
26. Kaufman TC, Seeger MA, Olsen G. 1990. Molecular and genetic organization of the antennapedia gene complex of Drosophila melanogaster. Adv Genet 27:309-362.
27. Kiernan JA. 2000. Formaldehyde, formalin, paraformaldehyde and glutaraldehyde: what they are and what they do. Microsc Today 1:8-12.
28. Kishimoto Y, Koshida S, Farutani-Seiki M, Kondo H. 2004. Zebrafish maternal-effect mutations causing cytokinesis defect without affecting mitosis or equatorial vasa deposition. Mech Dev 121:79-89.
29. Knaut H, Pelegri F, Bohmann K, Schwarz H, Nüsslein-Volhard C. 2000. Zebrafish vasa RNA but not its protein is a component of the germ plasm and segregates asymmetrically before germ line specification. J Cell Biol 149:875-888.
30. Köster RW, Fraser SE. 2001. Direct imaging of in vivo neuronal migration in the developing cerebellum. Curr Biol 11:1858-1863.
31. Lee KW, Ho CH, Webb SE, Miller AL. 2004. Characterization of mid-spindle microtubules during furrow positioning in early cleavage period zebrafish embryos. Zygote 12:221-230.
32. Lehmann R, Tautz D. 1994. In situ hybridization to RNA. In: Goldstein LSB, Fyrberg EA, editors. Drosophila melanogaster: practical uses in cell and molecular biology. San Diego: Academic Press. p 575-598.
33. Leung CF, Webb SE, Miller AL. 2000. On the mechanism of ooplasmic segregation in single-cell zebrafish embryos. Dev Growth Differ 42:29-40.
34. Lillie RD. 1955. Histopathologic technic and practical histochemistry. 3rd ed. New York: McGraw-Hill Book Co. 715 p.
35. Lu X, Li JM, Elemento O, Tavazoie S, Wieschaus E. 2009. Coupling of zygotic transcription to mitotic control at the Drosophila mid-blastula transition. Development 136:2101-2110.
36. Macdonald PM, Ingham P, Struhl G. 1986. Isolation, structure, and expression of even-skipped: a second pair-rule gene of Drosophila containing a homeobox. Cell 47:721-734.
37. Nüsslein-Volhard C, Wieschaus E. 1980. Mutations affecting segment number and polarity in Drosophila. Nature 287:795-801.
38. Orlando V, Strutt H, Paro R. 1997. Analysis of chromatin structure by in vivo formaldehyde cross-linking. In Methods: a companion to methods in enzymology. Methods 11:205-214.
39. Pelegri F. 2003. Maternal factors in zebrafish development. Dev Dyn 228:535-554.
40. Pelegri F, Knaut H, Maischein H-M, Schulte-Merker S, Nüsslein-Volhard C. 1999. A mutation in the zebrafish maternal-effect gene nebel affects furrow formation and vasa RNA localization. Curr Biol 9:1431-1440.
41. Pfaff KL, Straub CT, Chiang K, Bear DM, Zhou Y, Zon LI. 2007. The zebrafish cassiopeia mutant reveals that SIL is required for mitotic spindle organization. Mol Cell Biol 27:5887-5897.
42. Presnell JK, Schreibman MP. 1997. Humason's animal tissue techniques. 5th ed. Baltimore: The Johns Hopkins University Press. 600 p.
43. Puchtler H, Meloan SN. 1985. On the chemistry of formaldehyde fixation and its effects on immunohistochemical reactions. Histochemistry 82:201-204.
44. Renshaw S. 2007. Immunohistochemistry: methods express. Bloxham, UK: Scion Publishing Ltd. 250 p.
45. Roelink H, Porter JA, Chiang C, Tanabe Y, Chang DY, Beachy PA, Jessell TM. 1995. Floor plate and motor neuron induction by different concentrations of the amino-terminal cleavage product of sonic hedgehog autoproteolysis. Cell 81:445-455.
46. Romeis B. 1928. Guía Formulario de Técnica Histológica. 8a Edición. Barcelona: Labor S.A. 722 p.
47. Ruiz i Altaba A, Placzek M, Baldassare M, Dodd J, Jessell TM. 1995. Early stages of notochord and floor plate development in the chick embryo defined by normal and induced expression of HNF-3β. Dev Biol 170:299-313.
48. Schmiedeberg L, Skene P, Deaton A, Bird A. 2009. A temporal threshold for formaldehyde crosslinking and fixation. PloS ONE 4:1-5.
49. Schroeder MM, Gard DL. 1992. Organization and regulation of cortical microtubules during the first cell cycle of Xenopus eggs. Development 114:699-709.
50. Schulte-Merker S, Ho RK, Herrmann BG, Nüsslein-Volhard C. 1992. The protein product of the zebrafish homologue of the mouse T gene is expressed in nuclei of the germ ring and the notochord of the early embryo. Development 116:1021-1032.
51. Shao M, Lin Y, Liu Z, Zhang Y, Wang L, Liu C, Zhang H. 2012. GSK-3 activity is critical for the orientation of the cortical microtubules and the dorsoventral determination in zebrafish embryos. PLoS ONE 7:36665.
52. Sharp DJ, McDonald KL, Brown HM, Matthies HJ, Walczak C, Vale RD, Mitchison TJ, Scholey JM. 1999. The bipolar kinesin, KLP61F, cross-links microtubules within interpolar microtubule bundles of Drosophila embryonic mitotic spindles. J Cell Biol 144:125-138.
53. Shi SR, Cote RJ, Taylor CR. 2001. Antigen retrieval techniques: current perspectives. J Histochem Cytochem 49:931-937.
54. Solnica-Krezel L, Driever W. 1994. Microtubule arrays of the zebrafish yolk cell: organization and function during epiboly. Development 120:2443-2455.
55. Spencer VA, Davie JR. 2002. Isolation of protein cross-linked to DNA by formaldehyde. In Walker JM, editor. The protein protocols. Totowa, NJ: Humana Press Inc. p 753-757.
56. St Johnston D, Nüsslein-Volhard C. 1992. The origin of pattern and polarity in the Drosophila embryo. Cell 68:201-219.
57. Stachel S, Grunwald DJ, Myers PZ. 1993. Lithium perturbation and goosecoid expression identify a dorsal specification pathway in the pregastrula zebrafish. Development 117:1261-1274.
58. Strähle U, Jesuthasan S. 1993. Ultraviolet irradiation impairs epiboly in zebrafish embryos: evidence for microtubule-dependent mechanism of epiboly. Development 119:909-919.
59. Strähle U, Blader P, Henrique D, Ingham PW. 1993. Axial, a zebrafish gene expressed along the developing body axis, shows altered expression in cyclops mutant embryos. Genes Dev 7:1436-1446.
60. Streit A, Stern CD. 2001. Combined whole-mount in situ hybridization and immunohistochemistry in avian embryos. Methods 23:339-344.
61. Suzuki H, Maegawa S, Nishibu T, Sugiyama T, Yasuda K, Inoue K. 2000. Vegetal localization of the maternal mRNA encoding an EDEN-BP/Bruno-like protein in zebrafish. Mech Dev 93:205-209.
62. Sutherland BW, Toews J, Kast J. 2008. Utility of formaldehyde cross-linking and mass spectroscopy in the study of protein-protein interactions. J Mass Spectrometry 43:699-715.
63. Thisse B, Pflumio S, Fürthauer M, Loppin B, Heyer V, Degrave A, Woehle R, Lux A, Steffan T, Charbonnier XQ, Thisse C. 2001. Expression of the zebrafish genome during embryogenesis. ZFIN Direct Data Submission (http://zfin.org).
64. Thisse C, Thisse B. 2008. High-resolution in situ hybridization to whole-mount zebrafish embryos. Nat Protoc 3:59-69.
65. Weisblat DA, Kim SY, Stent GS. 1984. Embryonic origins of cells in the leech Helobdella triserialis. Dev Biol 104:65-85.
66. Wühr M, Chen Y, Dumont S, Groen AC, Niedelman DJ, Salic A, Mitchison TJ. 2008. Evidence for an upper limit to mitotic spindle length. Curr Biol 18:1256-1261.
67. Wühr M, Tan ES, Parker SK, Detrich HM III, Mitchison TJ. A model for cleavage plane determination in early amphibian and fish embryos. Curr Biol 20:2040-2045.
68. Yesbe T, Ge X, Pelegri F. 2007. The zebrafish maternal-effect gene cellular atoll encodes the centriolar component sas-6 and defects in its paternal function promotes whole genome duplication. Dev Biol 312:44-60.
69. Yoon C, Kawakami K, Hopkins N. 1997. Zebrafish vasa homologue RNA is localized to the cleavage planes of 2- and 4 cell-stage embryos and is expressed in the primordial germ cells. Development 124:3157-3165.