Improved native isolation of endogenous protein A-tagged protein complexes

BioTechniques

Volumn 54, Issue 4, 2013, Pages 213-216

  LaCava, John ^a   Chandramouli, Nagarajan ^a   Jiang, Hua ^a   Rout, Michael P ^a  

^a ROCKEFELLER UNIVERSITY   (United States)

Author keywords

Affinity capture; Affinity isolation; Competitive elution; Native elution; PrA; Protein A; SpA

Indexed keywords

HOLOENZYME; IMMUNOGLOBULIN G; MACROGOL; PROTEIN A;

ARTICLE; DENSITOMETRY; GEL FILTRATION CHROMATOGRAPHY; MASS SPECTROMETRY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN CLEAVAGE; PROTEIN INTERACTION; PROTEIN ISOLATION; PROTEIN MODIFICATION; ROOM TEMPERATURE; SACCHAROMYCES CEREVISIAE; ULTRAVIOLET SPECTROPHOTOMETRY;

EID: 84876485988     PISSN: 07366205     EISSN: 19409818     Source Type: Journal    
DOI: 10.2144/000114012     Document Type: Article

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