Immobilization of β-glucosidase on a magnetic nanoparticle improves thermostability: Application in cellobiose hydrolysis

Bioresource Technology

Volumn 135, Issue , 2013, Pages 2-6

  Verma, Madan L ^a   Chaudhary, Rajneesh ^a   Tsuzuki, Takuya ^a   Barrow, Colin J ^a   Puri, Munish ^a  

^a DEAKIN UNIVERSITY   (Australia)

Author keywords

Biofuel; Cellulase; Iron oxide; Lignocellulose; Nanobiotechnology

Indexed keywords

ASPERGILLUS; BIOFUELS; ENZYMES; FOURIER TRANSFORM INFRARED SPECTROSCOPY; HYDROLYSIS; IRON OXIDES; NANOBIOTECHNOLOGY; TRANSMISSION ELECTRON MICROSCOPY;

BIOFUEL PRODUCTION; CELLULASE; IMMOBILIZED ENZYME; IMMOBILIZED NANOPARTICLE; LIGNOCELLULOSE; MAGNETIC NANO-PARTICLES; MICHAELIS CONSTANTS; TRANSMISSION ELECTRON MICROSCOPY (TEM);

NANOPARTICLES;

BETA GLUCOSIDASE; CELLOBIOSE; GLUCOSE; SUPERPARAMAGNETIC IRON OXIDE NANOPARTICLE;

BIOFUEL; ENZYME ACTIVITY; FUNGUS; GLUCOSE; HYDROLYSIS; IMMOBILIZATION; IRON OXIDE; MAGNETIC PROPERTY; NANOTECHNOLOGY; OIL PRODUCTION; PARTICULATE MATTER; PH; TEMPERATURE EFFECT;

ARTICLE; ASPERGILLUS NIGER; CATALYSIS; COVALENT BOND; ENZYME ACTIVITY; ENZYME BINDING; ENZYME IMMOBILIZATION; ENZYME STABILITY; HYDROLYSIS KINETICS; INFRARED SPECTROSCOPY; MICHAELIS CONSTANT; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN HYDROLYSIS; TEMPERATURE DEPENDENCE; TRANSMISSION ELECTRON MICROSCOPY;

ASPERGILLUS NIGER;

BETA-GLUCOSIDASE; BIOCATALYSIS; CELLOBIOSE; ENZYME STABILITY; ENZYMES, IMMOBILIZED; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; KINETICS; MAGNETITE NANOPARTICLES; NITROPHENYLGALACTOSIDES; RECYCLING; SUBSTRATE SPECIFICITY; TEMPERATURE;

EID: 84876474057     PISSN: 09608524     EISSN: 18732976     Source Type: Journal    
DOI: 10.1016/j.biortech.2013.01.047     Document Type: Article

References (15)

1. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976, 72:248-254.
2. Chauve M., Mathis H., Huc D., Casanave D., Monot F., Ferreira N. Comparative kinetic analysis of two fungal beta-glucosidases. Biotechnol. Biofuels 2010, 3:2010.
3. Cho E.J., Jung S., Kim H.J., Lee Y.G., Nam K.C., Lee H.J., Bae H.J. Co-immobilization of three cellulases on Au-doped magnetic silica nanoparticles for the degradation of cellulose. Chem. Commun. 2012, 48(6):886-888.
4. Jung Y.R., Shin H.Y., Song Y.S., Kim S.B., Kim S.W. Enhancement of immobilized enzyme activity by pretreatment of β-glucosidase with cellobiose and glucose. J. Ind. Eng. Chem. 2011, 18:702-706.
5. Puri M., Abraham R.E., Barrow C.J. Biomass production: prospects, challenges and feedstock in Australia. Renew. Sustain. Energy Rev. 2012, 16:6022-6031.
6. Puri M., Barrow C.J., Verma M.L. Enzyme immobilization on nanomaterials for biofuel production 2013, Trends Biotechnol. 10.1016/j.tibtech.2013.01.002.
7. Singh R.K., Zhang Y.W., Nguyen N.P.T., Jeya M., Lee J.K. Covalent immobilization of β-1,4-glucosidase from Agaricus arvensis onto functionalized silicon oxide nanoparticles. Appl. Microbiol. Biotechnol. 2011, 89(2):337-344.
8. Sorensen A., Lübeck P.S., Lübeck M., Teller P.J., Ahring B.K. β-Glucosidases from a new Aspergillus species can substitute commercial β-glucosidases for saccharification of lignocellulosic biomass. Can. J. Microbiol. 2011, 57:638-650.
9. Takahashi M., Konishi Y., Takeda T. Biochemical characterization of Magnaporthe oryzae β-glucosidases for efficient β-glucan hydrolysis. Appl. Microbiol. Biotechnol. 2011, 91:1073-1082.
10. Tu M., Zhang X., Kurabi A., Gilkes N., Mabee W., Saddler J. Immobilization of β-glucosidase on Eupergit C for lignocellulose hydrolysis. Biotechnol. Lett. 2006, 28(3):151-156.
11. Verma M.L., Barrow C.J., Kennedy J.F., Puri M. Immobilization of β-d-galactosidase from Kluyveromyces lactis on functionalized silicon dioxide nanoparticles: characterization and lactose hydrolysis. Int. J. Biol. Macromol. 2012, 50:432-437.
12. Verma M.L., Barrow C.J., Puri M. Nanobiotechnology as a novel paradigm for enzyme immobilisation and stabilisation with potential applications in biodiesel production. Appl. Microbiol. Biotechnol. 2013, 97(1):23-39.
13. Wang P., Hu X., Cook S., Hwang H.M. Influence of silica-derived nano-supporters on cellobiase after immobilization. Appl. Biochem. Biotechnol. 2009, 158(1):88-96.
14. Xue R., Woodley J.M. Process technology for multi-enzymatic reaction systems. Bioresour. Technol 2012, 115:183-195.
15. Zhou Z., Zhang Y. Biosynthesis of β-glucosidase-silk fibrion nanoparticles conjugates and enzymatic characteristics. Adv. Mater. Res. 2011, 175-176:186-191.