Genotoxic effects of the herbicide Roundup® in the fish Corydoras paleatus (Jenyns 1842) after short-term, environmentally low concentration exposure

Environmental Monitoring and Assessment

Volumn 185, Issue 4, 2013, Pages 3201-3207

  De Castilhos Ghisi, Nédia ^a   Cestari, Marta Margarete ^b  

^a STATE UNIVERSITY OF MARINGÁ   (Brazil)

^b FEDERAL UNIVERSITY OF PARANÁ   (Brazil)

Author keywords

Comet assay; Fish; Glyphosate; Piscine micronucleus test; Water pollution

Indexed keywords

AGRICULTURAL YIELDS; COMET ASSAYS; GENOTOXIC EFFECTS; GLYPHOSATES; LOW CONCENTRATIONS; MICRONUCLEUS TEST; NEGATIVE CONTROL; TRANSGENIC CROPS;

FISH; WATER POLLUTION; WEED CONTROL;

HERBICIDES;

DNA; GLYPHOSATE; HERBICIDE; DRUG DERIVATIVE; GLYCINE; MUTAGENIC AGENT;

CONCENTRATION (COMPOSITION); FISH; GENOTOXICITY; GLYPHOSATE; HERBICIDE; PESTICIDE; POLLUTION EFFECT; POLLUTION EXPOSURE; WATER POLLUTION;

ANIMAL CELL; ARTICLE; COMET ASSAY; CONTROLLED STUDY; CORYDORAS PALEATUS; DNA DAMAGE; ENVIRONMENTAL EXPOSURE; FISH; GENOTOXICITY; INTERMETHOD COMPARISON; LIVER CELL; MICRONUCLEUS TEST; NONHUMAN; WATER ANALYSIS; WATER POLLUTION; ANIMAL; BIOASSAY; CATFISH; DOSE RESPONSE; WATER POLLUTANT;

CORYDORAS PALEATUS; PISCES;

ANIMALS; BIOLOGICAL ASSAY; CATFISHES; COMET ASSAY; DNA DAMAGE; DOSE-RESPONSE RELATIONSHIP, DRUG; GLYCINE; HERBICIDES; MICRONUCLEUS TESTS; MUTAGENS; WATER POLLUTANTS, CHEMICAL;

MLCS; MLOWN;

EID: 84876337129     PISSN: 01676369     EISSN: 15732959     Source Type: Journal    
DOI: 10.1007/s10661-012-2783-x     Document Type: Article

References (39)

1. Ayllon, F.; Garcia-Vazquez, E. (2000). Introduction of micronuclei and other nuclear abnormalities in European minnow Phoxinus phoxinus and mollie Poecilia latipinna: an assessment of the fish micronucleus test. Mutation Research, 476, 177-186.
2. Belpaeme, K.; Cooreman, K.; Kirsch-Volders, M. (1998). Development and validation of the in vivo alkaline comet assay for detecting genomic damage in marine flatfish. Mutation Research, 415(3), 167-184.
3. Boettcher, M.; Grund, S.; Keiter, S.; Kosmehl, T.; Reifferscheid, G.; Seitz, N.; et al. (2010). Comparison of in vitro and in situ genotoxicity in the Danube River by means of the comet assay and the micronucleus test. Mutation Research, 700, 11-17.
4. Canadian Council on Animal Care (2003) Guidelines on: laboratory animal facilities - characteristics, design and development, Ottawa ON Canada, p. 115. http://www.ccac.ca. Accessed 7 Sep 2011.
5. Cavalcante, M.; Martinez, R.; Sofia, S. (2008). Genotoxic effects of Roundup on the fish Prochilodus lineatus. Mutation Research, 655(1-2), 41-46.
6. Çavas, T. (2011). In vivo genotoxicity evaluation of atrazine and atrazine-based herbicide on fish Carassius auratus using the micronucleus test and the comet assay. Food and Chemical Toxicology, 49, 1431-1435.
7. Çavas, T.; Könen, S. (2007). Detection of cytogenetic and DNA damage in peripheral erythrocytes of goldfish (Carassius auratus) exposed to a glyphosate formulation using the micronucleus test and the comet assay. Mutagenesis, 22, 263-268.
8. Cestari, M. M.; Lemos, P. M. M.; Oliveira-Ribeiro, C. A.; Costa, J. R. M. A.; Pelletier, E.; Ferraro, M. V. M.; et al. (2004). Genetic damage induced by trophic doses of lead in the neotropical fish Hoplias malabaricus (Characiformes, Erythrinidae) as reveled by the comet assay and chromosomal aberrations. Genetic and Molecular Biology, 27(2), 270-274.
9. CEE - Comunidade Econômica Européia (1980). Directiva 80/778/CEE relativa à qualidade de águas destinadas ao consumo humano. Jornal Oficial da Comunidade Européia, n. L299, 20 p. http://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri=celex:31980l0778:pt:html. Accessed 10 Dec 2009.
10. Conners, A. J.; Jacobs, J. M. E. (1999). Genetic engineering of crops as potential source of genetic hazard in the human diet. Mutation Research, 443, 223-234.
11. Cox, C. (1998). Glyphosate factsheet. Journal of Pesticide Reform, 108(3), 3-17.
12. Dixon, D. R.; Pruski, A. M.; Dixon, L. R.; Jha, A. N. (2002). Marine invertebrate eco-genotoxicology: a methodological overview. Mutagenesis, 17, 495-507.
13. Ferraro, M. V. M.; Fenocchio, A. S.; Mantovani, M. S.; Cestari, M. M.; Ribeiro, C. A. O. (2004). Mutagenic effects of tributyltin (TBT) and inorganic lead (PbII) on the fish H. malabaricus as evaluated using the comet assay and piscine micronucleous and chromosome aberrations tests. Genetic and Molecular Biology, 27(1), 103-107.
14. Ghisi, N. C.; Ramsdorf, W. A.; Ferraro, M. V. M.; Almeida, M. I. M.; Ribeiro, C. A. O.; Cestari, M. M. (2010). Evaluation of genotoxicity in Rhamdia quelen (Pisces, Siluriformes) after sub-chronic contamination with Fipronil. Environmental Monitoring and Assessment, 180, 589-599. doi: 10.1007/s10661-010- 1807-7.
15. Gomez, S. E. (1992). Concentracion letal de oxigeno dissuelto para Corydoras paleatus y Pimelodella laticeps (Pisces, Siluriformes). Revista del Museo Argentino de Ciencias Naturales Bernardino Rivadavia, 7(2), 31-45.
16. Governmental Decree 518 of the Ministry of Health - Ministerio da Saúde-Portaria MS n°518/2004 publicado no Diário Oficial da União de 26 de março de 2004. http://www.casan.com.br/docs/ PortariaMS518-2004.pdf. Accessed 18 February 2012
17. Grisolia, C. K. (2002). A comparison between mouse and fish micronucleus test using cyclophosphamide, mitomycin C and various pesticides. Mutation Research, 518, 145-150.
18. Guilherme, S.; Gaivão, I.; Santos, M. A.; Pacheco, M. (2010). European eel (Anguilla anguilla) genotoxic and pro-oxidant responses following short-term exposure to Roundup® - a glyphosate-based herbicide. Mutagenesis, 25, 523-530.
19. Hartmann, A.; Elhajouji, A.; Kiskinis, E.; Poetter, F.; Martus, H. J.; Fjallman, A.; et al. (2001). Use of the alkaline comet assay for industrial genotoxicity screening: comparative investigation with the micronucleus test. Food and Chemical Toxicology, 39, 843-858.
20. Heddle, J. A. (1973). A rapid in vivo test for chromosome damage. Mutation Research, 18, 187-192.
21. Jiraungkoorskul, W.; Upatham, E. S.; Kruatrachue, M.; Sahaphong, S.; Vichasri-Grams, S.; Pokethitiyook, P. (2003). Biochemical and histopathological effects of glyphosate herbicide on Nile tilapia (Oreochromis niloticus). Environmental Toxicology, 18(4), 260-267.
22. Klobucar, G. I. V.; Pavlica, M.; Erben, R.; Papes, D. (2003). Application of the micronucleus and comet assays to mussel Dreissena polymorpha haemocytes for genotoxicity monitoring of freshwater environments. Aquatic Toxicology, 64, 15-23.
23. Kobayashi, H.; Sugiyama, C.; Morikawa, Y.; Hayashi, M.; Sofuni, T. (1995). A comparison between manual microscopic analysis and computerized image analysis in the single cell gel electrophoresis. Mammalian Mutagenicity Study Group Communications, 3, 103-115.
24. Marc, J.; Mulner-Lorillon, O.; Bellé, R. (2004). Glyphosate-based pesticides affect cell cycle regulation. Biology of the Cell, 96, 245-249.
25. Molinari, M. (2000). Cell cycle checkpoints and their inactivation in human cancer. Cell Proliferation, 33, 261-274.
26. Peruzzo, P. J.; Porta, A. A.; Ronco, A. E. (2008). Levels of glyphosate in surface waters, sediments and soils associated with direct sowing soybean cultivation in north pampasic region of Argentina. Environmental Pollution, 156, 61-66.
27. R Development Core Team (2011). R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. http://www.R-project.org/.
28. Rossi, S. C.; Silva, M. D. S.; Piancini, L. D. S.; Ribeiro, C. A. O.; Cestari, M. M.; Assis, H. C. S. (2011). Sublethal effects of waterborne herbicides in tropical freshwater fish. Bulletin of Environmental Contamination and Toxicology, 87, 603-607. doi: 10.1007/s00128-011-0397-6.
29. Russo, C.; Rocco, L.; Morescalchi, M. A.; Stingo, V. (2004). Assessment of environments stress by the micronucleus test and the comet assay on the genome of teleost populations from two natural environments. Ecotoxicology and Environmental Safety, 57, 168-174.
30. Sasaki, Y. F.; Tsuda, S.; Izumiyama, F.; Nishidate, E. (1997). Detection of chemically induced DNA lesions in multiple mouse organs (liver, lung, spleen, kidney, and bone marrow) using the alkaline single cell gel electrophoresis (comet) assay. Mutation Research, 388, 33-44.
31. Schmid, W. (1975). The micronucleus test. Mutation Research, 31, 9-15.
32. Speit, G.; Hartmann, A. (1999). The comet assay (single-cell gel test), a sensitive genotoxicity test for the detection of DNA damage and repair. In D. S. Henderson (Ed.), Methods in molecular biology DNA repair protocols - eukaryotic systems (pp. 203-211). Totowa: Hum Press. 113.
33. Stewart, Z. A.; Westfall, M. D.; Pietenpol, J. A. (2003). Cell-cycle dysregulation and anticancer therapy. Trends Pharmacology Sciences, 24, 139-145.
34. Struger, J.; Thompson, D.; Staznik, B.; Martin, P.; McDaniel, T.; Marvin, C. (2008). Occurrence of glyphosate in surface waters of southern Ontario. Bulletin of Environmental Contamination Toxicology, 80, 378-384. doi: 10.1007/s00128-008-9373-1.
35. Szarek, J.; Siwicki, A.; Andrzejewska, A.; Terech-Majewska, E.; Banaszkiewicz, T. (2000). Effects of the herbicide Roundup™ on the ultrastructural pattern of hepatocytes in carp (Cyprinus carpio). Marine Environmental Research, 50, 263-266.
36. United States Environmental Protection Agency. (2009). National primary drinking water regulations, EPA 816-F-09-004. http://www.epa.gov/safewater/. Accessed 28 June 2011.
37. Vasquez, M. Z. (2010). Combining the in vivo comet and micronucleus assays: a practical approach to genotoxicity testing and data interpretation. Mutagenesis, 25(2), 187-199.
38. ZHEW (2002). Monitoring network in fruit plantations and investigation of drainage water. 1996-2001. Zuiveringsschap Hollandse Eilanden en Waarden, Dordrecht (in Dutch). As cited in Horth et al. 2004. Survey of glyphosate in groundwaters and surface waters in Europe. Final update report 2003/2004, p. 71.
39. Zorita, I.; Ortiz-Zarragoitia, M.; Apraiz, I.; Cancio, I.; Orbea, A.; Soto, M.; et al. (2008). Assessment of biological effects of environmental pollution along the NW Mediterranean Sea using red mullets as sentinel organisms. Environmental Pollution, 153(1), 157-168.