Improving PCR efficiency for accurate quantification of 16S rRNA genes

Journal of Microbiological Methods

Volumn 93, Issue 2, 2013, Pages 148-152

  Callbeck, Cameron M ^a   Sherry, Angela ^b   Hubert, Casey R J ^b   Gray, Neil D ^b   Voordouw, Gerrit ^a   Head, Ian M ^b  

^a UNIVERSITY OF CALGARY   (Canada)

^b NEWCASTLE UNIVERSITY   (United Kingdom)

Author keywords

16S rRNA gene; PCR efficiency; QPCR

Indexed keywords

BACTERIAL DNA; PETROLEUM; RNA 16S;

16S RRNA GENE; ACCURACY; ARTICLE; BACTERIUM; BIODEGRADATION; CLONE; ENVIRONMENTAL ENRICHMENT; GENE AMPLIFICATION; GENE TARGETING; MARINOBACTER; METHANOGENIC BACTERIUM; METHANOMICROBIALES; METHANOSAETACEAE; METHANOSARCINACEAE; MICROBIAL COMMUNITY; NONHUMAN; POLYMERASE CHAIN REACTION; POPULATION ABUNDANCE; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; SMITHELLA; STANDARD; SYNTROPHUS;

ARCHAEA; BACTERIA; ENVIRONMENTAL MICROBIOLOGY; MICROBIOLOGICAL TECHNIQUES; REAL-TIME POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S;

MARINOBACTER; METHANOMICROBIALES; METHANOSAETACEAE; METHANOSARCINACEAE; SMITHELLA; SYNTROPHUS;

EID: 84876333963     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2013.03.010     Document Type: Article

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