A method for correcting standard-based real-time PCR DNA quantitation when the standard's polymerase reaction efficiency is significantly different from that of the unknown's

Analytical and Bioanalytical Chemistry

Volumn 402, Issue 9, 2012, Pages 2713-2725

  Irwin, Peter L ^a   Nguyen, Ly Huong T ^a   Chen, Chin Yi ^a   Uhlich, Gaylen A ^a   Paoli, George C ^a  

^a EASTERN REGIONAL RESEARCH CENTER   (United States)

Author keywords

16S rDNA or rRNA gene; DNA quantitation; Real time PCR or qPCR

Indexed keywords

AMPLICONS; CELL EXTRACTS; CONCENTRATION DEPENDENCE; CYCLE NUMBER; DNA CONCENTRATION; DNA SOLUTIONS; MATHEMATICAL PROCEDURES; PATHOGEN DETECTION; PURIFICATION PROCESS; QUANTITATIVE POLYMERASE CHAIN REACTION; REACTION EFFICIENCY; REAL-TIME PCR; RRNA GENES; STANDARD CURVES; STANDARD SOLUTIONS;

DNA; GENES; RNA; STANDARDS;

POLYMERASE CHAIN REACTION;

BACTERIAL DNA; DNA DIRECTED DNA POLYMERASE; RIBOSOME DNA; RNA 16S;

ARTICLE; BACTERIUM; CHEMISTRY; EVALUATION; GENETICS; MATHEMATICS; METHODOLOGY; REAL TIME POLYMERASE CHAIN REACTION; STANDARD; THEORETICAL MODEL;

BACTERIA; DNA, BACTERIAL; DNA, RIBOSOMAL; DNA-DIRECTED DNA POLYMERASE; MATHEMATICS; MODELS, THEORETICAL; REAL-TIME POLYMERASE CHAIN REACTION; REFERENCE STANDARDS; RNA, RIBOSOMAL, 16S;

EID: 84862803227     PISSN: 16182642     EISSN: 16182650     Source Type: Journal    
DOI: 10.1007/s00216-012-5737-9     Document Type: Article

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