Cloning, expression and characterization of a Trichinella spiralis serine protease gene encoding a 35.5kDa protein

Experimental Parasitology

Volumn 134, Issue 2, 2013, Pages 148-154

  Wang, Bin ^a   Wang, Zhong Quan ^a   Jin, Jing ^a   Ren, Hui Jun ^a   Liu, Li Na ^a   Cui, Jing ^a  

^a ZHENGZHOU UNIVERSITY   (China)

Author keywords

Immune protection; Invasion; Serine protease; T. spiralis

Indexed keywords

PARASITE ANTIBODY; RECOMBINANT PROTEIN; SERINE PROTEINASE; VACCINE;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CONTROLLED STUDY; DEVELOPMENTAL STAGE; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC TRANSCRIPTION; HUMAN; HUMAN CELL; IMMUNITY; IMMUNIZATION; IMMUNOLOCALIZATION; IN VITRO STUDY; INTESTINE EPITHELIUM CELL; LARVA; MALE; MOLECULAR CLONING; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN TRANSPORT; TRICHINELLA SPIRALIS; TRICHINOSIS; TSPSP 1.2 GENE; WESTERN BLOTTING;

ANIMALS; ANTIBODIES, HELMINTH; ANTIGENS, HELMINTH; BLOTTING, WESTERN; CELL LINE, TUMOR; CLONING, MOLECULAR; FEMALE; FLUORESCENT ANTIBODY TECHNIQUE; GENE EXPRESSION REGULATION, ENZYMOLOGIC; HELMINTH PROTEINS; HUMANS; MALE; MICE; MICE, INBRED BALB C; RANDOM ALLOCATION; RECOMBINANT PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SERINE PROTEASES; SPECIFIC PATHOGEN-FREE ORGANISMS; SWINE; TRICHINELLA SPIRALIS; TRICHINELLOSIS;

ESCHERICHIA COLI; MUS; TRICHINELLA SPIRALIS;

EID: 84876323951     PISSN: 00144894     EISSN: 10902449     Source Type: Journal    
DOI: 10.1016/j.exppara.2013.03.004     Document Type: Article

References (36)

1. Appleton J.A., Schain L.R., McGregor D.D. Rapid expulsion of Trichinella spiralis in suckling rats: mediation by monoclonal antibodies. Immunology 1988, 65:487-492.
2. Arden S.R., Smith A.M., Booth M.J., Tweedie S., Gounaris K., Selkirk M.E. Identification of serine/threonine protein kinases secreted by Trichinella spiralis infective larvae. Mol. Biochem. Parasitol. 1997, 90:111-119.
3. Bien J., Nareaho A., Varmanen P., Gozdzik K., Moskwa B., Cabaj W., Nyman T.A., Savijoki K. Comparative analysis of excretory-secretory antigens of Trichinella spiralis and Trichinella britovi muscle larvae by two-dimensional difference gel electrophoresis and immunoblotting. Proteome Sci. 2012, 10:10.
4. Bolás-Fernandez F., Corral-Bezara L.D. TSL-1 antigens of Trichinella: an overview of their potential role in parasite invasion, survival and serodiagnosis of trichinellosis. Res. Vet. Sci. 2006, 81:297-303.
5. Criado-Fornelio A., de Armas-Serra C., Gimenez-Pardo C., Casado-Escribano N., Jimenez-Gonzalez A., Rodriguez-Caabeiro F. Proteolytic enzymes from Trichinella spiralis larvae. Vet. Parasitol. 1992, 45:133-140.
6. Cui J., Wang Z.Q., Zhu W., Zhang R.G. Seroepidemiologicl study of Trichinella spiralis infection in central China. Helminthologia 1999, 36:235-239.
7. Cwiklinski K., Meskill D., Robinson M.W., Pozio E., Appleton J.A., Connolly B. Cloning and analysis of a Trichinella pseudospiralis muscle larva secreted serine protease gene. Vet. Parasitol. 2009, 59:268-271.
8. Denkers E.Y., Wassom D.L., Hayes C.E. Characterization of Trichinella spiralis antigens sharing an immunodominant, carbohydrate-associated determinant distinct from phosphorylcholine. Mol. Biochem. Parasitol. 1990, 41:241-249.
9. Despommier D. A circular thermal migration device for the rapid collection of large numbers of intestinal helminths. J. Parasitol. 1973, 59:933-935.
10. Despommier D.D. How does Trichinella spiralis make itself at home?. Parasitol. Today 1998, 14:318-323.
11. Dzik J.M. Molecules released by helminth parasites involved in host colonization. Acta. Biochim. Pol. 2006, 53:33-64.
12. Feng S., Wu X., Wang X., Bai X., Shi H., Tang B., Liu X., Song Y., Pascal B., Wang F., Zhao Y., Liu M. Vaccination of mice with an antigenic serine protease-like protein elicits a protective immune response against Trichinella spiralis infection. J. Parasitol. 2012, http://dx.doi.org/10.1645/12-46.1.
13. Harlow E., Lane D. Antibodies: A laboratory manual Cold Spring Harbor Laboratory 1988, Cold Spring Harbor, New York.
14. Inaba T., Sato H., Kamiya H. Monoclonal IgA antibody mediated expulsion of Trichinella from the intestine of mice. Parasitology 2003, 126:591-598.
15. Jiang P., Wang Z.Q., Cui J., Zhang X. Comparison of artificial digestion and Baermann's methods for detection of Trichinella spiralis pre-encapsulated larvae in muscles with low-level infections. Foodborne Pathog. Dis. 2012, 9:27-31.
16. Li F., Cui J., Wang Z.Q., Jiang P. Factors affecting the sensitivity of artificial digestion and its optimization for inspection of Trichinella spiralis in meat. Foodborne Pathog. Dis. 2010, 7:879-885.
17. ManWarren T., Gagliardo L., Geyer J., McVay C., Pearce-Kelling S., Appleton J. Invasion of intestinal epithelia in vitro by the parasitic nematode Trichinella spiralis. Infect. Immun. 1997, 65:4806-4812.
18. Marston F.A. The purification of eukaryotic polypeptides synthesized in Escherichia coli. Biochem. J. 1986, 240:1-12.
19. McVay C.S., Tsung A., Appleton J. Participation of parasite surface glycoproteins in antibody-mediated protection of epithelia against Trichinella spiralis. Infect. Immun. 1998, 66:1941-1945.
20. McVay C.S., Bracken P., Gagliardo L.F., Appleton J. Antibodies to tyvelose exhibit multiple modes of interference with the epithelial niche of Trichinella spiralis. Infect. Immun. 2000, 68:1912-1918.
21. Moczon T., Wranicz M. Trichinella spiralis: proteinases in the larvae. Parasitol. Res. 1999, 85:47-58.
22. Nagano I., Wu Z., Takahashi Y. Functional genes and proteins of Trichinella spp. Parasitol. Res. 2009, 104:197-207.
23. Robinson M.W., Connolly B. Proteomic analysis of the excretory-secretory proteins of the Trichinella spiralis L1 larva, a nematode parasite of skeletal muscle. Proteomics 2005, 5:4525-4532.
24. Robinson M.W., Greig R., Beattie K.A., Lamont D.J., Connolly B. Comparative analysis of the excretory-secretory proteome of the muscle larva of Trichinella pseudospiralis and Trichinella spiralis. Int. J. Parasitol. 2007, 37:139-148.
25. Robinson M.W., Massie D.H., Connolly B. Secretion and processing of a novel multi-domain cystatin-like protein by intracellular stages of Trichinella spiralis. Mol. Biochem. Parasitol. 2007, 151:9-17.
26. Romaris F., North S.J., Gagliardo L.F., Butcher B.A., Ghosh K., Beiting D.P., Panico M., Arasu P., Dell A., Morris H.R., Appleton J.A. A putative serine protease among the excretory-secretory glycoproteins of L1 Trichinella spiralis. Mol. Biochem. Parasitol. 2002, 122:149-160.
27. Takada N., Tada T. Collection of newborn larvae of Trichinella spiralis in vitro. Jpn. J. Parasitol 1988, 7:251-253.
28. Todorova V.K. Proteolytic enzymes secreted by larval stage of the parasitic nematode Trichinella spiralis. Folia Parasitol. 2000, 47:141-145.
29. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 1979, 76:4350-4354.
30. Wang Z.Q., Cui J., Wei H.Y., Han H.M., Zhang H.W., Li Y.L. Vaccination of mice with DNA vaccine induces the immune response and partial protection against T. spiralis infection. Vaccine 2006, 24:1205-1212.
31. Wang S.W., Cui J., Wang Z.Q., Wang L. Effect of immune sera on the invasion of T. spiralis infective larvae into intestinal epithelial cells and their development in vitro. Chin. J. Parasitol. Parasit. Dis. 2010, 28:81-85.
32. Wang L., Cui J., Wang S.W., Wang Z.Q. Observation on invasion of intestinal epithelial cells in vitro by Trichinella spiralis larvae and development. Chin. Pathog. Biol. 2010, 5:901-903.
33. Wang S.W., Wang Z.Q., Cui J. Protein change of intestinal epithelial cells induced in vitro by Trichinella spiralis infective larvae. Parasitol. Res. 2011, 108:593-599.
34. Wang Z.Q., Wang L., Cui J. Proteomic analysis of Trichinella spiralis proteins in intestinal epithelial cells after culture with their larvae by shotgun LC-MS/MS approach. J. Proteomics 2012, 75:2375-2383.
35. Wisnewski N., McNeil M., Grieve R.B., Wassom D.L. Characterization of novel fucosyland tyvelosyl-containing glycoconjugates from Trichinella spiralis muscle stage larvae. Mol. Biochem. Parasitol. 1993, 61:25-35.
36. Yang J., Gu Y., Yang Y., Wei J., Wang S., Cui S., Pan J., Li Q., Zhu X. Trichinella spiralis: immune response and protective immunity elicited by recombinant paramyosin formulated with different adjuvants. Exp. Parasitol. 2010, 124:403-408.