A sensitive and specific multiplex PCR approach for sex identification of ursine and tremarctine bears suitable for non-invasive samples

Molecular Ecology Resources

Volumn 13, Issue 3, 2013, Pages 362-368

  Bidon, Tobias ^a   Frosch, Christiane ^b,c   Eiken, Hans G ^d   Kutschera, Verena E ^a   Hagen, Snorre B ^d   Aarnes, Siv G ^d   Fain, Steven R ^e   Janke, Axel ^f   Hailer, Frank ^a  

^a SENCKENBERG DEUTSCHES ENTOMOLOGISCHES INSTITUT   (Germany)

^b SENCKENBERG RESEARCH INSTITUTE   (Germany)

^c Frankfurt Zoological Society   (Germany)

^d NORWEGIAN INSTITUTE OF BIOECONOMY RESEARCH   (Norway)

^e US Fish and Wildlife Service   (United States)

^f GOETHE UNIVERSITY   (Germany)

Author keywords

Bears; Molecular sexing; Multiplex PCR; Non invasive; Ursidae; Ursus arctos

Indexed keywords

KRUPPEL LIKE FACTOR; PRIMER DNA; ZINC FINGER PROTEIN, X LINKED; ZINC FINGER PROTEIN, X-LINKED;

ANIMAL; ARTICLE; BEAR; CAPILLARY ELECTROPHORESIS; CHEMISTRY; DNA SEQUENCE; FECES; FEMALE; GENETIC MARKER; GENETICS; HAIR; MALE; METHODOLOGY; MOLECULAR GENETICS; MULTIPLEX POLYMERASE CHAIN REACTION; NUCLEOTIDE SEQUENCE; SENSITIVITY AND SPECIFICITY; SEX DETERMINATION; SPECIES DIFFERENCE; Y CHROMOSOME;

ANIMALS; BASE SEQUENCE; DNA PRIMERS; ELECTROPHORESIS, CAPILLARY; FECES; FEMALE; GENETIC MARKERS; HAIR; KRUPPEL-LIKE TRANSCRIPTION FACTORS; MALE; MOLECULAR SEQUENCE DATA; MULTIPLEX POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS, DNA; SEX DETERMINATION ANALYSIS; SPECIES SPECIFICITY; URSIDAE; Y CHROMOSOME;

URSIDAE; URSINAE; URSUS ARCTOS;

EID: 84876323351     PISSN: 1755098X     EISSN: 17550998     Source Type: Journal    
DOI: 10.1111/1755-0998.12072     Document Type: Article

References (28)

1. Aasen E, Medrano JF (1990) Amplification of the ZFY and ZFX genes for sex identification in humans, cattle, sheep and goats. Nature Biotechnology, 8, 1279-1281.
2. Ahlering M, Hailer F, Roberts M, Foley C (2011) A Simple and accurate method to sex savannah, forest and asian elephants using noninvasive sampling techniques. Molecular Ecology Resources, 11, 831-834.
3. Amstrup SC, Garner GW, Cronin MA, Patton JC (1993) Sex identification of polar bears from blood and tissue samples. Canadian Journal of Zoology, 71, 2174-2177.
4. Andreassen R, Schregel J, Kopatz A et al. (2012) A forensic DNA profiling system for Northern European brown bears (Ursus arctos). Forensic Science International: Genetics, 6, 798-809.
5. ® duo DNA quantification kit for simultaneous quantification of total human and human male DNA and detection of PCR inhibitors in biological samples. Journal of Forensic Sciences, 54, 305-319.
6. Fang J, Yu Z, Lian, M et al. (2012) Knockdown of zinc finger protein, X-linked (ZFX) inhibits cell proliferation and induces apoptosis in human laryngeal squamous cell carcinoma. Molecular and Cellular Biochemistry, 360, 301-307.
7. Farrell LE, Roman J, Sunquist ME (2000) Dietary separation of sympatric carnivores identified by molecular analysis of scats. Molecular Ecology, 9, 1583-1590.
8. Frosch C, Dutsov A, Georgiev, G, Nowak C (2011) Case report of a fatal bear attack documented by forensic wildlife genetics. Forensic Science International: Genetics, 5, 342-344.
9. Jobling MA, Gill P (2004) Encoded evidence: DNA in forensic analysis. Nature Reviews Genetics, 5, 739-751.
10. Kohn M, Knauer F, Stoffella A, Schröder W, Pääbo S (1995) Conservation genetics o the European brown bear - a study using excremental PCR of nuclear and mitochondrial sequences. Molecular Ecology, 4, 95-103.
11. Kopatz A, Eiken HG, Hagen SB et al. (2012) Connectivity and population subdivision at the fringe of a large brown bear (Ursus arctos) population in North Western Europe. Conservation Genetics, 13, 681-692.
12. Millar CD, Lambert DM, Anderson S, Halverson JL (1996) Molecular sexing of the communally breeding pukeko: an important ecological tool. Molecular Ecology, 5, 289-293.
13. Morin PA, Chambers KE, Boesch C, Vigilant L (2001) Quantitative polymerase chain reaction analysis of DNA from noninvasive samples for accurate microsatellite genotyping of wild chimpanzees (Pan troglodytes verus). Molecular Ecology, 10, 1835-1844.
14. Murphy MA, Waits LP, Kendall KC (2003) The influence of diet on faecal DNA amplification and sex identification in brown bears (Ursus arctos). Molecular Ecology, 12, 2261-2265.
15. Pagès M, Maudet C, Bellemain E, Taberlet P, Hughes S, Hänni C (2009) A system for sex determination from degraded DNA: a useful tool for palaeogenetics and conservation genetics of ursids. Conservation Genetics, 10, 897-907.
16. Rosel PE (2003) PCR-based sex determination in Odontocete cetaceans. Conservation Genetics, 4, 647-649.
17. Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Bioinformatics Methods and Protocols: Methods in Molecular Biology (eds Krawetz S & Misener S), pp. 365-386. Humana Press, Totowa, NJ.
18. Shaw CN, Wilson PJ, White BN (2003) A reliable molecular method of gender determination for mammals. Journal of Mammalogy, 84, 123-128.
19. Taberlet P, Mattock H, Dubois-Paganon C, Bouvet J (1993) Sexing free-ranging brown bears Ursus arctos using hairs found in the field. Molecular Ecology, 2, 399-403.
20. Taberlet P, Griffin S, Goossens B (1996) Reliable genotyping of samples with very low DNA quantities using PCR. Nucleic Acids Research, 24, 3189-3194.
21. Taberlet P, Camarra JJ, Griffin S, Uhrès E et al. (1997) Noninvasive genetic tracking of the endangered Pyrenean brown bear population. Molecular Ecology, 6, 869-876.
22. Tringali MD, Davis MC, Bolen EE, Sullivan JG, Haubold EM (2008) Simultaneous use of the X- and Y-chromosome genes SMCX, SMCY, and DBY for sex determination in the Florida manatee (Trichechus manatus latirostris). Marine Mammal Science, 24, 218-224.
23. Waits LP, Paetkau D (2005) Noninvasive genetic sampling tools for wildlife biologists. A review of applications and recommendations for accurate data collection. Journal of Wildlife Management, 69, 1419-1433.
24. Wandeler P, Smith S, Morin PA, Pettifor RA, Funk SM (2003) Patterns of nuclear DNA degeneration over time-a case study in historic teeth samples. Molecular Ecology, 12, 1087-1093.
25. Wasser SK, Houston CS, Koehler GM, Cadd GG, Fain SR (1997) Techniques for application of faecal DNA methods to field studies of Ursids. Molecular Ecology, 6, 1091-1097.
26. Woods JG, Paetkau D, Lewis D, McLellan BN, Proctor M, Strobeck C (1999) Genetic tagging of free-ranging black and brown bears. Wildlife Society Bulletin, 27, 616-627.
27. Xu X, Lin L, Zhang Z, Shen F, Zhang L, Yue B (2008) A reliable, non-invasive PCR method for giant panda (Ailuropoda melanoleuca) sex identification. Conservation Genetics, 9, 739-741.
28. Yamamoto K, Tsubota T, Komatsu T et al. (2002) Sex identification of Japanese black bear, Ursus thibetanus japonicus, by PCR based on amelogenin gene. The Journal of Veterinary Medical Science, 64, 505-508.