Developmental validation of the Quantifiler® Duo DNA Quantification kit for simultaneous quantification of total human and human male DNA and detection of PCR inhibitors in biological samples

Journal of Forensic Sciences

Volumn 54, Issue 2, 2009, Pages 305-319

  Barbisin, Maura ^a   Fang, Rixun ^a   O'Shea, Cristin E ^a   Calandro, Lisa M ^a   Furtado, Manohar R ^a   Shewale, Jaiprakash G ^a  

^a APPLIED BIOSYSTEMS   (United States)

Author keywords

DNA analysis; DNA quantification; DNA typing; Forensic science; Human DNA; Human male DNA; Real time PCR; Ribonuclease P RNA component H1; Sex determining region Y

Indexed keywords

GENOMIC DNA; HEMATIN; HUMIC ACID; RIBONUCLEASE P; RNA;

ACCURACY; ARTICLE; CONTROLLED STUDY; CORRELATION ANALYSIS; DNA DETERMINATION; DNA FINGERPRINTING; FEMALE; FORENSIC SCIENCE; HUMAN; HUMAN EXPERIMENT; MALE; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; SHORT TANDEM REPEAT; SRY GENE; VALIDATION PROCESS;

AFRICAN CONTINENTAL ANCESTRY GROUP; ANIMALS; CHROMOSOMES, HUMAN, Y; DNA; DNA DEGRADATION, NECROTIC; DNA FINGERPRINTING; EUROPEAN CONTINENTAL ANCESTRY GROUP; FEMALE; GENETICS, POPULATION; HEMIN; HUMANS; HUMIC SUBSTANCES; MALE; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; RIBONUCLEASE P; RNA; SENSITIVITY AND SPECIFICITY; SPECIES SPECIFICITY; TANDEM REPEAT SEQUENCES;

EID: 60849123137     PISSN: 00221198     EISSN: 15564029     Source Type: Journal    
DOI: 10.1111/j.1556-4029.2008.00951.x     Document Type: Article

References (15)

1. Moretti TR, Baumstark AL, Defenbaugh DA, Keys KM, Smerick JB, Budowle B. Validation of short tandem repeats (STRs) for forensic usage: performance testing of fluorescent multiplex STR systems and analysis of authentic and simulated forensic samples. J Forensic Sci 2001 46 : 647 60.
2. Butler JM. Forensic DNA typing, 2nd edn. Burlington : Elsevier Academic Press, 2005.
3. Walsh PS, Varlaro J, Reynolds R. A rapid chemiluminescent method for quantitation of human DNA. Nucleic Acid Res 1992 20 : 5061 5.
4. Green RL, Roinestad IC, Boland C, Hennessy LK. Developmental validation of the Quantifiler™ real-time PCR kits for the quantitation of human nuclear DNA samples. J Forensic Sci 2005 50 : 809 25.
5. Walker JA, Hedges DJ, Perodeau BP, Landry KE, Stoilova N, Laborde ME, et al. Multiplex polymerase chain reaction for simultaneous quantitation of human nuclear, mitochondrial, and male Y-chromosome DNA; application in human identification. Anal Biochem 2005 337 : 89 97.
6. Horsman KM, Hickey JA, Cotton RW, Landers JP, Maddox LO. Development of a human specific real-time PCR assay for the simultaneous quantitation of total genomic and male DNA. J Forensic Sci 2006 51 : 758 65.
7. Nicklas JA, Buel E. Simultaneous determination of total human and male DNA using a duplex real-time PCR assay. J Forensic Sci 2006 51 : 1005 15.
8. Swango KL, Hudlow WR, Timken MD, Buoncristiani MR. Developmental validation of a multiplex qPCR assay for assessing the quantity and quality of nuclear DNA in forensic samples. Forensic Sci Int 2007 170 : 35 45.
9. Budowle B, Smith J, Moretti T, DiZinno J. DNA typing protocols: molecular biology and forensic analysis. Natik : Eaton Publishing, 2000 41 2.
10. Mulero JJ, Chang CW, Calandra LM, Green RL, Li Y, Johnson CL, et al. Development and validation of the AmpFlSTR® Yfiler™ PCR amplification kit: a male specific, single amplification 17 Y-STR multiplex system. J Forensic Sci 2006 51 : 64 75.
11. TM PCR amplification kit: a MiniSTR multiplex for the analysis of degraded and/or PCR inhibited DNA. J Forensic Sci 2008 53 (4 838 52.
12. Holland PM, Abramson RD, Watson R, Gelfand DH. Detection of specific polymerase chain reaction product by utilizing the 5′ to 3′ exonuclease activity of Thermus aquaticus DNA polymerase. Proc Natl Acad Sci USA 1991 88 (16 7276 80.
13. Scientific Working Group on DNA Analysis Methods (SWGDAM). Revised Validation Guidelines issued by the Scientific Working Group on DNA Analysis Methods (SWGDAM). Forensic Sci Commun 2004 6 (3). Available at http://www.fbi.gov/hq/lab/fsc/backissu/july2004/standards/2004-03-standards02. htm#perfcheck.
14. Shewale JG, Schneida E, Wilson J, Walker JA, Batzer MA, Sinha SK. Human genomic DNA quantitation system, H-Quant: development and validation for use in forensic casework. J Forensic Sci 2007 52 : 364 70.
15. Timken MD, Swango KL, Orrego C, Buoncristiani MR. A duplex real-time qPCR assay for the quantification of human nuclear and mitochondrial DNA in forensic samples: implications for quantifying DNA in degraded samples. J Forensic Sci 2005 50 : 1044 60.