Extraction, derivatization, and determination of metabolome in human macrophages

Journal of Separation Science

Volumn 36, Issue 8, 2013, Pages 1418-1428

  Cheng, Jianhua ^a   Che, Nanying ^b   Li, Haijing ^a   Ma, Kunpeng ^a   Wu, Shengming ^a   Fang, Junjian ^a   Gao, Rong ^a   Liu, Jiexin ^a   Yan, Xianzhong ^a   Li, Chuanyou ^b   Dong, Fangting ^a  

^a National Center of Biomedical Analysis   (China)

^b CAPITAL MEDICAL UNIVERSITY   (China)

Author keywords

Extraction; Gas chromatography time of flight mass spectrometry; Human macrophages; Metabolome

Indexed keywords

CORRELATION COEFFICIENT; ENDOGENOUS METABOLITES; EXTRACTION CONDITIONS; FAVORABLE CONDITIONS; HUMAN MACROPHAGES; METABOLOMES; MULTIVARIATE STATISTICAL TECHNIQUES; MYCOBACTERIUM TUBERCULOSIS;

AMINO ACIDS; BIOMOLECULES; EXTRACTION; MASS SPECTROMETRY; METABOLITES; MULTIVARIANT ANALYSIS; OPTIMIZATION; TUBES (COMPONENTS);

MACROPHAGES;

2 OXOGLUTARIC ACID; ALANINE; AMINO ACID; ARABINITOL; ASPARTIC ACID; CREATININE; CYSTEINE; GLUTAMIC ACID; GLYCERIC ACID; GLYCEROPHOSPHOLIPID; GLYCINE; HYDROXYPROLINE; ICE; ISOLEUCINE; LACTIC ACID; LEUCINE; MALIC ACID; METHIONINE; ORNITHINE; OXALIC ACID; PHENYLALANINE; PHOSPHORIC ACID; PROLINE; PYROGLUTAMIC ACID; SERINE; SODIUM CHLORIDE; SUCCINIC ACID; THREONINE; UREA; VALINE;

ACCURACY; ARTICLE; BACTERIAL STRAIN; BATH; CONTROLLED STUDY; DERIVATIZATION; GAS CHROMATOGRAPHY; HUMAN; HUMAN CELL; INFANT; MACROPHAGE; MALE; METABOLITE; METABOLOME; METABOLOMICS; MYCOBACTERIUM TUBERCULOSIS; PHOTOCHEMICAL QUENCHING; PRIORITY JOURNAL; PROCESS OPTIMIZATION; SOLVENT EXTRACTION; TIME OF FLIGHT MASS SPECTROMETRY; TUBERCULOSIS; VALIDATION PROCESS;

CELLS, CULTURED; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; HUMANS; MACROPHAGES; METABOLOME; SPECTROPHOTOMETRY, ULTRAVIOLET;

MYCOBACTERIUM TUBERCULOSIS;

EID: 84876287388     PISSN: 16159306     EISSN: 16159314     Source Type: Journal    
DOI: 10.1002/jssc.201201158     Document Type: Article

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